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Serratia marcescens for degrading sodium lauryl sulfate and use thereof

A technology based on sodium lauryl sulfate and Serratia, applied in the direction of microbial-based methods, bacteria, restoration of contaminated soil, etc., can solve the problem of no report on the degradation ability of Serratia marcescens

Active Publication Date: 2017-02-15
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Screening efficient strains to degrade SDS has become a research trend and hotspot, but there is no report on the degradation ability of Serratia marcescens in the current research on biodegradation of SDS

Method used

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  • Serratia marcescens for degrading sodium lauryl sulfate and use thereof
  • Serratia marcescens for degrading sodium lauryl sulfate and use thereof
  • Serratia marcescens for degrading sodium lauryl sulfate and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The separation and screening method of Serratia degrading sodium lauryl sulfate comprises the following steps:

[0022] 1) 20 parts of soil samples were taken from the periphery of a washing powder manufacturer in Kaifeng City, as materials for the isolation and screening of degrading bacteria;

[0023] 2) Select LB medium to isolate the strains of the collected soil samples by the dilution coating plate method, and obtain a total of 162 strains of bacteria;

[0024] 3) Using LB medium as the base medium, prepare enriched medium containing different concentrations of SDS (50mg / L, 100mg / L, 150mg / L, 200mg / L); streak inoculate the 162 strains of bacteria that have been obtained Cultivate overnight at 37°C on rich medium plates containing different concentrations of SDS, and conduct preliminary screening of the strains by observing the growth of the strains, and screen out 5 strains of bacteria that are stable and tolerant to high concentrations of SDS;

[0025] 4) Inocula...

Embodiment 2

[0050] The application of Serratia (CCTCC NO:M2015246) in degrading SDS, specifically: Take Serratia marcescens (CCTCC NO:M2015246) to expand culture, collect the bacteria, add the bacteria and a small amount of glucose to the SDS-containing in the sewage.

Embodiment 3

[0052] The preparation steps for degrading SDS are as follows: take Serratia marcescens (CCTCC NO:M2015246) to expand and cultivate, collect the bacteria, and add glycerol to freeze (conventional operation).

[0053]

[0054]

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PUM

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Abstract

The invention discloses Serratia marcescens for degrading sodium lauryl sulfate and a use thereof and belongs to the technical field of environmental microbial application. The Serratia marcescens strain is named as Serratia marcescens GW-1, has a preservation number of CCTCC NO: M2015246, is isolated from the soil rich in SDS, is in a shape of a nearly spherical short rod, has a small volume, is gram-negative and does not produce spores. The colony cultured on a LB plate at 37 DEG C is non-transparent, smooth, moist and white and has the raised center and the irregular edge. The Serratia marcescens cultured on the LB plate at 28 DEG C can produce a red pigment. The Serratia marcescens GW-1 (CCTCC NO: M2015246) realizes effective degradation of sodium dodecyl sulfate and can be used to prepare a preparation for degradation of SDS.

Description

technical field [0001] The invention relates to a Serratia bacterium capable of efficiently degrading sodium lauryl sulfate and an application thereof, belonging to the technical field of environmental microorganism application. Background technique [0002] Surfactant (Surface active agent, surfant) refers to a class of compounds that contain both hydrophilic groups and hydrophobic groups in the molecule, and can significantly reduce the surface tension of the medium at a lower concentration. At present, artificially synthesized surfactants are widely used in industrial and agricultural production, such as detergents, agricultural chemical sprays, etc., resulting in a large amount of surfactants remaining in the environment, causing increasingly serious environmental pollution. Studies have shown that surfactants may damage cell tissue, cause human allergies, premature aging of crops, pollute water quality and poison aquatic organisms. [0003] Sodium dodecyl sulfate (sodi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34B09C1/10C12R1/43A62D101/28
CPCA62D3/02A62D2101/28B09C1/10C02F3/34C12N1/205C12R2001/43
Inventor 王刚王淼黄秋斌张影刘鸣张浩
Owner HENAN UNIVERSITY
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