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Polypeptide valid target in Ropalidia fasciata insects and application thereof in preventing and treating cerebral thrombosis

A technology of effective parts and wasps, which is applied in the field of preparation of effective parts of polypeptides in insects of the genus Vespa, can solve the problems of low level of industrial production support and failure to form large-scale production capacity, and reduce cerebral edema and permeability , the effect of prolonging the breathing time

Inactive Publication Date: 2014-03-19
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with developed countries, there is still a relatively obvious gap in the research and development of my country's TTS system, especially the low level of supporting industrial production, which has not yet formed a large-scale production capacity

Method used

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  • Polypeptide valid target in Ropalidia fasciata insects and application thereof in preventing and treating cerebral thrombosis
  • Polypeptide valid target in Ropalidia fasciata insects and application thereof in preventing and treating cerebral thrombosis
  • Polypeptide valid target in Ropalidia fasciata insects and application thereof in preventing and treating cerebral thrombosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 : Preparation of effective fraction of Vespa venom with bell-bellies and hydrogel

[0035] 1.1 Collection of crude wasp venom with bell-bellies:

[0036] The bell-bellied Vespa was collected from Nalun Township, Guangnan County, Wenshan Prefecture, Yunnan Province. The bee specimen was identified as Ropalidia by Professor Yang Zizhong of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province (hereinafter referred to as: Engineering Research Center) fasciata Fabricius. Wasp venom collection device: use the auxiliary cover type bee venom collector modified by the engineering research center, wooden frame, 80 cm long, 50 cm wide; embedded with 4 pieces of 20 cm long / 33 cm wide poisonous glass; input voltage 10V , to output automatic intermittent pulsating voltage; the wooden frame is painted red with red paint in advance; the wooden frame is pre-smeared with 20 ml concentration...

Embodiment 2

[0041] Example 2 : Preparation of active parts of Vespa venom polypeptide and coating agent

[0042] 2.1 Collection of crude venom from Vespa rufica:

[0043] The wasp was collected from Jinghong County, Yunnan Province. The bee specimen was identified as Ropalidia ferruginea Fabricius by Professor Guo Yunjiao of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province. Wasp venom collection device: use the auxiliary cover type bee venom collector modified by the engineering research center, wooden frame, 80 cm long, 50 cm wide; embedded with 4 pieces of 20 cm long / 33 cm wide poisonous glass; input voltage 12V , output automatic intermittent pulsating voltage; method of collecting wasp venom: put the unpainted poison collector in front of the hive, and collect the poison for 30 minutes according to the routine poison collection procedures. Turn on the power switch of the venom collector, tap ...

Embodiment 3

[0048] Example 3 : New bell-bellied Vespa bee venom effective fraction and preparation of cataplasm

[0049] 3.1 Collection of crude venom from New Bell-bellied Vespa:

[0050] The new bell-bellied Vespa was collected from Cangyuan, Yunnan Province, and the bee body specimen was identified as Ropalidia artifex Saussure by Professor Yang Zizhong of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali College, Yunnan Province. The basic device for poison collection uses a sub-cap type bee venom collector modified by the engineering research center, with a wooden frame, 80 cm long and 50 cm wide; 4 pieces of poisonous glass with a length of 20 cm / width of 33 cm are embedded; the input voltage is 10V, Output automatic intermittent pulsating voltage; add a photodiode bulb bearing layer under the glass plate, use LED bulbs from Zhengzhou Hengguang Technology Co., Ltd., and connect to the battery with wires. Arrange the re...

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Abstract

The invention relates to a polypeptide valid target in Ropalidia fasciata insects and application thereof in preventing and treating cerebral thrombosis. The valid target is prepared by the following steps: dialyzing Ropalidia fasciata crude bee venom with a 30kDa dialysis membrane, and carrying out freeze-drying or vacuum concentration and drying on the dialysis solution. A series of pharmacodynamic experiments by people prove that the biotoxin polypeptide valid target and a transdermal absorption preparation thereof have obvious effects on preventing and treating cerebral thrombosis, restoring mobility of animals with cerebral palsy, preventing and treating platelet accumulation and relieving ischemic brain injury, and have better pharmacodynamic performance than Plavix, Xingnaojing injection and other first-tier medicines. The valid target is expected to be used for preparing innovative biochemical medicines for treating cardiovascular and cerebrovascular diseases and for preventing and treating ischemic cardiovascular and cerebrovascular diseases, such as myocardial infarction, cardiovascular thrombosis, cerebral thrombosis, cerebral infarction, acute / chronic cerebral apoplexy, and sequelae caused by cerebral infarction injury, including hemiplegia, hemidysesthesia, hemianopsia, facioplegia, paralysis lingualis, aphasia, pamplegia and the like.

Description

technical field [0001] The present invention relates to the technical field of medicine, in particular, the present invention relates to a preparation method and medical use of the effective part of the polypeptide in Vespa bellbellum insects. The effective part is obtained by dialyzing the crude bee venom of Vespa genus Bollina with a dialysis membrane of 25KDa, and the eluate is freeze-dried or concentrated and dried under reduced pressure. Through a series of pharmacological and pharmacodynamic experiments in animals, the inventors found that the effective part of the polypeptide has the effect of significantly preventing and treating cerebral thrombosis, restoring the activity ability of cerebral palsy animals, and reducing ischemic brain damage, and can be expected to be used in the preparation of anti-cardiac and cerebrovascular diseases. Vascular diseases include drugs, health care products, medical devices and daily chemicals for ischemic cerebrovascular disease and is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/34A61K38/17A61P9/10A61P7/02A61P9/00
CPCC07K14/43572A61K38/00
Inventor 何颖红巫秀美郑园园杨自忠李贵轲赵昱
Owner DALI UNIV
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