Method for establishing mouse castrating high-ferrum osteoporotic model

Abstract

The invention discloses a method for establishing a mouse castrating high-ferrum osteoporotic model. The method comprises the steps that an 8-week-old ICR female mouse is selected and bilateral oophorectomy is conducted on the mouse; one week after the oophorectomy is conducted, FAC is started to be injected in the abdominal cavity and the mouse castrating high-ferrum osteoporotic model is obtained after 7 weeks. Currently, ferric accumulation happens to most clinic postmenopausal women. Recently, many researches show that ferric accumulation is a high risk factor of osteoporosis. However, a traditional castrating osteoporotic model is normal in ferric level and can not completely simulate the postmenopausal women. Compared with a traditional model, the mouse castrating high-ferrum osteoporotic model established through the method is more close to clinic phenomena.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for constructing an animal experiment model, and in particular to a method for constructing a mouse castrated high iron osteoporosis model. Background technique [0002] The absorption process of iron in the body is quite complicated. Ferric iron in food is reduced to ferrous iron by intestinal cytochrome B (Dcytb) in the small intestine, and then transported to the villous epithelium of the small intestine by divalent cation transporter 1 (DMT1) In the cell, at this time, ferrous iron can be transported into the blood via ferroportin 1 (FPN1) at the base of the cell, and at the same time be oxidized to trivalent by ferroportin accessory protein (Hp), and combined with transferrin in the blood. The combined complex is transported to various tissues and organs along with the blood, and interacts with the cells in the tissue, and the intracellular steap3 is reduced to fer...

AI Technical Summary

Problems solved by technology

Therefore, the traditional mouse model of castrated osteoporosis cannot accurately reflect the high iron status of postmenopausal women

Other models of osteoporosis are not ideal, such as Beagle dogs, although there is menstrual flow, but the frequency is 2 times / year, the menstrual blood is too little, and the iron level in the body is not greatly affected after castration and menopause; The menstrual condition is closest to that of human beings, but it takes too long for the high-speed rail status to occur after castration and menopause. It takes a year or more, and the cost performance is not high.

High iron level is an independent risk factor for osteoporosis, but the traditional castration model cannot reflect this risk factor well

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