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Method for constructing tissue engineering cartilage

A technology of tissue engineering and construction methods, applied in medical science, prosthesis, etc., can solve problems such as abnormal and defective breathing

Inactive Publication Date: 2014-03-12
SHANGHAI TISSUE ENG LIFE SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main method of clinical repair of congenital microtia is to use costal cartilage to carve and form a human ear-like structure to repair the damaged position, which is often to remove the east wall to make up the west wall, and it is easy to cause defects such as pneumothorax or abnormal breathing caused by cartilage defects.

Method used

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  • Method for constructing tissue engineering cartilage
  • Method for constructing tissue engineering cartilage
  • Method for constructing tissue engineering cartilage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0050] Materials and methods:

[0051] 1. Preparation of pinna-shaped titanium alloy stent

[0052] A normal adult ear was scanned by CT scan, and a miniature version (2:1) of the auricle mold was prepared by 3D printing technology based on the scan data, and a three-dimensional structure of the auricle-shaped bracket was pressed out of titanium alloy ( figure 1 A), 75% alcohol for disinfection.

[0053] 2. Preparation of cartilage acellular matrix membrane

[0054] Take fresh adult porcine auricular cartilage, cut it into a cylindrical shape with a diameter of 9 mm with a corneal trephine, and cut it into circular slices with a thickness of 10 μm with a cryostat, put it in 1% SDS (Shanghai Shenggong) solution, and shake it overnight on a shaker. Sections were rinsed several times with distilled water, and freeze-dried in a freeze dryer (Virtis, USA) for later use ( figure 1 B).

[0055] 3. Preparation of Gelatin / PCL nanofiber electrospun membrane

[0056] The ratio of Ge...

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Abstract

The invention discloses a method for constructing tissue engineering cartilage. The method includes the steps of firstly, adding a membrane to an auricular-shaped support with a three-dimensional structure; secondly, adding cartilage cell suspension to the membrane; thirdly, adding a membrane to the cartilage cell suspension generated in the second step; fourthly, repeatedly executing the second step and the third step to obtain a composite; fifthly, culturing the composite in vitro to obtain the tissue engineering cartilage.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a method for constructing tissue engineering cartilage by using a prefabricated auricle-shaped bracket with a three-dimensional structure. Background technique [0002] Cartilage is an avascular tissue. Because of its low self-repair ability, cartilage damage or deformity has always been a major problem in clinical treatment. Tissue engineering technology provides a new treatment method for cartilage defect repair. In addition to solving the two key problems of cells and materials in cartilage construction, how to construct cartilage tissue with a three-dimensional fine structure is a difficult point. There are various forms of cartilage defects in the body, such as sports injury defects of articular cartilage, especially microtia, which is very common in clinical practice, which requires that tissue engineered cartilage can also be preformed into different shapes. At present,...

Claims

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Application Information

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IPC IPC(8): A61F2/00
Inventor 张文杰薛继鑫龚轶一郑蕊周广东刘伟曹谊林
Owner SHANGHAI TISSUE ENG LIFE SCI
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