Method for preparing oligopeptide through mixed enzymolysis and fermentation of fish meal processing waste liquid and vegetable proteins
A plant protein and mixed enzymatic hydrolysis technology, which is applied in food processing, animal feed, animal feed, etc., can solve the problems of unfavorable large-scale production, reduced oligopeptide content, and cumbersome preparation steps, etc., and achieves remarkable enzymatic hydrolysis effect and nutritional Balanced and fully enzymatic effect
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Embodiment 1
[0020] 400 kg of crushed cotton meal, 400 kg of rapeseed meal and 200 kg of fresh white wine grains, mix with 1000 kg of concentrated silver carp and fish meal waste liquid, adjust pH to 5.5-7.0, add compound enzyme preparation, stir evenly, enzymatically In 2 hours, the enzymatic hydrolysis temperature is controlled within the range of 30℃-40℃. Enzyme preparations include neutral protease 2000u / g, papain 3000u / g, glucanase 1000u / g, and cellulase 1000u / g. After the enzymolysis, 5 kg of Bacillus subtilis, 4 kg of feed yeast, and 5 kg of Lactobacillus plantarum were added, mixed and fermented for 64 hours. Aeration is taken every 6-8 hours for 40s-80s, the fermentation temperature is controlled below 45℃ by turning the material and cooling measures, and the closed anaerobic fermentation is carried out after 48 hours of fermentation. After fermentation is completed, it is dried, crushed and packaged.
[0021] Silver carp is generally the first choice fish for fish processing. Ther...
Embodiment 2
[0023] 500 kg of crushed cotton meal, 500 kg of rapeseed meal, and 100 kg of fresh white distiller’s grains, mix with 600 kg of concentrated fish meal waste liquid, adjust pH to 5.5-7.0, add compound enzyme preparation, stir evenly, enzymolysis for 2 hours, The temperature of enzymolysis is controlled within the range of 30℃-40℃. Enzyme preparations include neutral protease 1000u / g, alkaline protease 1000u / g, papain 2000u / g, glucanase 4000u / g, and cellulase 1000u / g. After the enzymolysis, 3 kg of Bacillus subtilis, 2 kg of feed yeast, and 3 kg of Lactobacillus plantarum were added, mixed and fermented for 56 hours. Aeration is taken every 6-8 hours for 40s-80s, the fermentation temperature is controlled below 45℃ by turning the material and cooling measures, and the closed anaerobic fermentation is carried out after 48 hours of fermentation. After the fermentation is completed, it is directly sealed and vacuum packaged or dried, crushed and packaged.
Embodiment 3
[0025] 1000 kg of crushed cotton meal, 1000 kg of rapeseed meal, and 500 kg of fresh white distiller’s grains, mixed with 900 kg of concentrated fish meal waste liquid, adjusted to pH 5.5-7.0, added compound enzyme preparation, stirred evenly, enzymatic hydrolysis for 2.5 hours , The temperature of enzymolysis is controlled within the range of 30℃-40℃. Enzyme preparations include neutral protease 1000u / g, papain 2000u / g, pectinase 1000u / g, glucanase 4000u / g, and cellulase 1000u / g. After the enzymolysis, 6 kg of Bacillus subtilis, 5 kg of feed yeast, and 8 kg of Lactobacillus plantarum were added, mixed and fermented for 48 hours. Aeration is taken every 6-8 hours for 40s-80s, the fermentation temperature is controlled below 45°C by turning the material and cooling measures, and the closed anaerobic fermentation is carried out after 30 hours of fermentation. After the fermentation is completed, it is directly sealed and vacuum packaged.
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