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Method for lowering COD (Chemical Oxygen Demand) of oil refining wastewater by using dibutyl phthalate degrading strain

A technology of dibutyl phthalate and oil refining wastewater is applied in the field of environmental pollution and bio-enhancement, and can solve the problem of high COD in biochemical effluent

Inactive Publication Date: 2014-03-05
WUHAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The present invention aims to solve the problem of high COD in the biochemical effluent of oil refinery wastewater, and aims to provide a method for reducing COD in oil refinery wastewater by using dibutyl phthalate degrading bacteria, which can effectively reduce COD in oil refinery wastewater

Method used

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  • Method for lowering COD (Chemical Oxygen Demand) of oil refining wastewater by using dibutyl phthalate degrading strain
  • Method for lowering COD (Chemical Oxygen Demand) of oil refining wastewater by using dibutyl phthalate degrading strain
  • Method for lowering COD (Chemical Oxygen Demand) of oil refining wastewater by using dibutyl phthalate degrading strain

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Effect test

Embodiment 1

[0020] Isolation and Purification of Strain D8 and Its Degradation of Dibutyl Phthalate

[0021] The bacteria source was collected from the activated sludge of the sewage treatment system of Wuhan Branch of China Petroleum & Chemical Corporation. Take 2 ml of activated sludge and inoculate it into 100 mL of sterilized LB medium, activate and cultivate overnight at 30 °C and 150 r / min. Then take 2mL of the culture solution and transfer it to the inorganic salt medium containing 200 mg / L dibutyl phthalate, cultivate for 1 day under the same conditions, and gradually increase the concentration of dibutyl phthalate (according to 200mg / L L gradient increases). After the acclimatization is completed, the bacterial liquids are serially diluted, respectively spread on inorganic salt solid plates containing dibutyl phthalate, and cultured at 30° C. for 7 days. Pick a single colony and repeat the streaking 3-4 times. Pick out the strains with better degradation ability and store them...

Embodiment 2

[0026] Degradation of Dibutyl Phthalate in Oil Refinery Wastewater by Strain D8

[0027] Two sets of experiments were designed to investigate the removal effect of strain D8 on dibutyl phthalate in oil refinery wastewater: (1) oil refinery wastewater + a certain concentration of dibutyl phthalate; (2) inorganic salt medium + a certain concentration of dibutyl phthalate; concentration of dibutyl phthalate. Inoculate strain D8 into sterilized oil refinery wastewater and inorganic salt medium with an inoculum volume of 2 vol%, and add dibutyl phthalate with a concentration of 50-400 mg / L to two sets of Erlenmeyer flasks respectively, at 30°C And 150r / min shaker culture, after 30h, take a sample and detect the content of dibutyl phthalate, the result is as follows figure 2 shown. Depend on figure 2 It can be seen that the removal effect of strain D8 on dibutyl phthalate in oil refinery wastewater is consistent with the removal effect of dibutyl phthalate in inorganic salt med...

Embodiment 3

[0029] Removal of COD in wastewater after air flotation in refinery biochemical station by strain D8

[0030] The strain D8 was inoculated into the sterilized waste water of a biochemical station of an oil refinery after air flotation with an inoculation amount of 2 vol%, and cultured on a shaker at 30°C and 150 r / min. Samples were taken every 12 hours to detect the COD in the waste water. is the abscissa, and the COD concentration in the wastewater is plotted as the ordinate, the result is as follows image 3 shown. Depend on image 3 It can be seen that, from 0 to 24 hours, the COD in the wastewater decreased rapidly with the prolongation of the treatment time. After 24 hours, the COD in the wastewater decreased from 930 mg / L to 176 mg / L, and the removal rate was as high as 81%. The experimental results show that: the strain D8 can quickly adapt to the actual oil refinery wastewater environment, not only can grow and reproduce with dibutyl phthalate as the substrate, but a...

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Abstract

The invention relates to a method for lowering the COD (Chemical Oxygen Demand) of oil refining wastewater by using a dibutyl phthalate degrading strain. The dibutyl phthalate degrading strain D8 provided by the invention is collected in CCTCC (China Center for Type Culture Collection), the collection date is March 20, 2012, and the collection number is CCTCC NO: M2012088. The strain D8 is a Gram-negative bacterium, is yellow in colony and neat in edge, is circular, smooth and wet, has a bulged center and can grow and propagate in a manner of taking dibutyl phthalate as a unique carbon source and energy. The strain not only can effectively degrade dibutyl phthalate in the oil refining wastewater, but also can effectively degrade other organic substances and wastewater biochemical intermediates in the wastewater so as to lower the COD of the oil refining wastewater. The strain can be applied to the bioaugmentation treatment of the oil refining wastewater and has a broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of environmental pollution bioaugmentation. In particular, it relates to a method for reducing COD in oil refinery wastewater by using dibutyl phthalate degrading bacteria. Background technique [0002] In recent years, with the rapid development of my country's economy, petroleum and its products have been used in various fields of the national economy and people's daily life. The demand for oil products has increased rapidly, and my country's oil refining capacity has now ranked second in the world. The resulting volume of refinery wastewater is also increasing. [0003] Refinery wastewater not only has a large amount of discharge, but also has complex components and various types. The COD content in the wastewater is high and the treatment is difficult. With the increase of environmental protection, the national and local governments have formulated a series of policies and regulations, and the pollutio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F103/06
Inventor 颜家保余永登杨洋许龙龙陈佩武文丽袁东东
Owner WUHAN UNIV OF SCI & TECH
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