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Identification method for rape honey doped in acacia honey

A technology of rapeseed honey and acacia honey, which is applied in the field of food science, can solve problems such as the difficulty of distinguishing acacia honey, and achieve the effects of excellent selectivity and sensitivity, high sensitivity, and strong feasibility

Active Publication Date: 2014-02-19
西安众天食品有限责任公司
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  • Application Information

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Problems solved by technology

The current quality control of honey mainly focuses on the two major components of sugar and polyphenols, and these two types of components exist in both acacia honey and rapeseed honey, which makes it quite difficult to distinguish acacia honey from acacia honey mixed with rapeseed honey

Method used

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  • Identification method for rape honey doped in acacia honey
  • Identification method for rape honey doped in acacia honey
  • Identification method for rape honey doped in acacia honey

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Embodiment Construction

[0027] Further description will be given below in conjunction with the accompanying drawings and embodiments.

[0028] Accurately weigh 0.0330 mg gallic acid, 0.0205 mg protocatechuic acid, 0.2700 mg p-hydroxybenzoic acid, 0.12 mg chlorogenic acid, 0.0190 mg caffeic acid, 0.0270 mg syringic acid, 0.0150 mg p ?Coumaric acid, 0.0540 mg ferulic acid, 0.0520 mg ellagic acid and 0.0640 mg rosmarinic acid ten kinds of phenolic acid standard, dissolved in chromatographically pure methanol and dilute to 10 mL, as the reference solution. Filter through a 0.45 μm microporous membrane and analyze by HPLC?ECD. Using ZORBAX SB?C18 (250 mm×4.6 mm, 5 μm), the mobile phase is: A: methanol B: 0.1% acetic acid aqueous solution; gradient elution method: 0 min? 10 min? 20 min? 25 min? 30 min ?50 min?60 min?70 min, Methanol: 5%?15%?15%?17%?30%? 40%?55%?70%, 1.0% acetic acid aqueous solution: 95%?85%?85%? 83%? 70%? 60%? 45%? 30%; flow rate: 1.0 ml / min; detection voltage: 0.9 V; detection waveleng...

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Abstract

The invention discloses an identification method for rape honey doped in acacia honey. Firstly, chemical compounds of phenolic acids are extracted from honey by means of resin, and chromatographically pure methyl alcohol of a metered volume is utilized to prepare a test solution; then certain amounts of ten kinds of phenolic acid comparison products are respectively taken, and chromatographically pure methyl alcohol of a metered volume is utilized to prepare a comparison product solution. The determination method comprises the steps that the comparison product solution and the test solution are respectively absorbed to be injected into a high performance liquid chromatograph, a chromatogram is measured and recorded by making the comparison products as reference substances, an HPLC-ECD fingerprint spectrum of the acacia honey is obtained, an HPLC-ECD fingerprint spectrum of the rape honey and an HPLC-ECD fingerprint spectrum of the acacia honey containing the rape honey are obtained by means of the same method, the three spectrums are compared, and by combining a chemometrics method, whether the rape honey is doped in the acacia honey is identified. The identification method is reasonable in design, good in separation effect and high in sensitivity and feasibility, and resolves an important technical problem in acacia honey adulteration identification and quality control.

Description

technical field [0001] The invention relates to a method for identifying rape honey mixed with acacia honey, in particular to a method for identifying rape honey mixed with acacia honey by using high performance liquid chromatography fingerprints combined with a chemometric method, and belongs to the field of food science. Background technique [0002] Honey is a natural sweet substance that is fully brewed by bees after collecting nectar, secretions or honeydew from plants, combining with their own secretions. Honey is known as "the essence of a hundred flowers" and is recognized as a natural food with various biological activities. Flavonoids, phenolic acids, organic acids, proteins, amino acids and other trace active substances in honey are closely related to the types of nectar plants. An important component of efficacy such as disease. The quality of honey depends on the type of nectar source and its chemical composition. Because the sensory characteristics of honey ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 曹炜王锦梅赵静程妮雷兴生
Owner 西安众天食品有限责任公司
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