Environmental-friendly biological organic fertilizer and preparation method thereof
A bio-organic fertilizer and green technology, applied in the field of agriculture, can solve problems such as fertility decline, air pollution, waste of land, etc., and achieve the effects of simple preparation method, prevention of pests and diseases, and low production cost
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[0035] The preparation method of the microbial fermentation compound agent is specifically:
[0036] In the first step, Coriolus versicolor and Bacillus subtilis are respectively inoculated into the seed medium, and cultured on a shaker at 36°C to 37°C at 150 to 170 rpm for 16 to 20 hours until OD 600 =0.7~0.9, stop culturing, and use as seed solution;
[0037] In the second step, the seed liquid of Coriolus versicolor and Bacillus subtilis is mixed in a volume ratio of 0.5-0.8:0.3-0.5, more preferably 0.6:0.4, and then sterilized at high temperature according to the amount of 5%-10%. In the final fermentation medium liquid, the temperature of 25 ℃ ~ 30 ℃ is static for 7 ~ 10 days. When the pH value of the fermentation liquid is 3.5 ~ 4.0, the number of living microorganisms reaches 10 8 ~10 9 Individual / ml or more, the microbial fermentation compound agent is obtained.
[0038] Wherein, the seed medium in the seed medium is composed of 10 grams of glucose, 5 grams of lacto...
Embodiment 1
[0065] The preparation of embodiment 1 microbial fermentation compound agent
[0066]Coriolus versicolor (Dalian Wangli Wild Ganoderma lucidum Co., Ltd.) and Bacillus subtilis (Shanghai Fusheng Biotechnology Co., Ltd.) were inoculated into the seed medium respectively, and the seed medium in the seed medium consisted of 10 gram of glucose, 5g of lactose, 5g of sodium chloride, 10g of peptone, 10g of yeast extract and 1 liter of water, the pH value of which is 6.5, at 37°C, 170rpm, after 20 hours of shaker cultivation, until OD 600 =0.9, stop cultivating, as seed liquid, after the seed liquid of Coriolus versicolor and Bacillus subtilis is mixed with volume ratio 0.6:0.4, in the fermented medium liquid after the high-temperature sterilization according to the amount access of 10%, The fermentation medium is composed of 10g of glucose, 5g of lactose, 5g of sodium acetate, 10g of peptone, 10g of yeast extract and 1 liter of water. The pH of the fermentation broth is 4.0, and the...
Embodiment 2
[0067] Embodiment 2 green biological organic fertilizer 1
[0068] Put 150g of Rhododendron vine, 250g of peach branch, 350g of Cnidium chinensis, 350g of vetex, 250g of vitex leaf, 150g of Torreya chinensis, 150g of saponin, 150g of mountain bean root, 150g of betel nut, 150g of Chinese toon bark, 350g of gypsum, 250g of castor bean, and astragalus 250g and 150g of white hair rattan are dried, respectively pulverized to below 40 mesh, sent to a mixer for stirring and mixing, the moisture content of wheat straw is adjusted to 20wt%, the moisture content of chicken manure is adjusted to 60wt%, and then 3.5kg of chicken manure is taken Mix with 7kg of crushed wheat straw, 0.6kg of the previously obtained Chinese medicine mixture, 0.8kg of medical stone, and 2kg of distiller's grains, and pile them into triangular piles for open-air composting. The open-air temperature is controlled at 20°C, and the obtained mixture is adjusted. The water content is between 55wt%, and the carbon-...
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