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Method for preparing cancer cell targeted cell-like micro-capsules

A cell-mimicking and targeting technology, which is applied in the field of preparing cancer cell-targeting cell-mimicking microcapsules, can solve the problems of limited popularization and application, poor ability to target cancer tissues and cells, and poor biocompatibility, and achieve saving Raw materials, good targeting and killing of cancer cells, fast preparation effect

Active Publication Date: 2014-02-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, they often have poor biocompatibility, are easily recognized and eliminated by the human immune system, and have the disadvantages of poor ability to target cancer tissues and cells, which limit their promotion and application.

Method used

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  • Method for preparing cancer cell targeted cell-like micro-capsules
  • Method for preparing cancer cell targeted cell-like micro-capsules
  • Method for preparing cancer cell targeted cell-like micro-capsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Add 20 μL of cytochalasin b solution (concentration: 1 mg / mL) to the culture dish of human umbilical vein endothelial cells, incubate at 37°C for 30 minutes, wash with phosphate buffer three times, digest with trypsin, and collect cells by centrifugation ; The obtained cells were vortexed for 1 min; the supernatant collected by centrifugation was the cell-like microcapsule suspension.

[0024] Disperse 200 μg of the cell-mimetic microcapsules obtained above in 1 mL of phosphate buffer; add 10 μL of 1 mg / mL cell membrane red dye DiI to it, mix well, and incubate for 15 min; centrifuge and wash the obtained cell-mimetic microcapsules several times to remove Unlabeled dye; for fluorescence photographs of cell-mimetic microcapsules dispersed in phosphate buffer see figure 1 .

[0025] 2) Take 1 mg of the cell-like microcapsule suspension obtained in step 1) and add it to the nucleic acid aptamer solution modified with aldehyde groups at the end, so that the concentratio...

Embodiment 2

[0028] Same as Example 1, the difference lies in step 3), take 200 μg of the cell-like microcapsules obtained in step 2) and add it to the doxorubicin hydrochloride solution, so that the concentration of doxorubicin hydrochloride is 80 μg / mL, incubate at 37°C for 12 hours; centrifuge Wash three times with phosphate buffered saline. The cancer cell-targeted cell-like microcapsule loaded with doxorubicin hydrochloride was obtained. According to the absorbance at 543nm in the ultraviolet spectrogram, the drug loading capacity of the microcapsules is 2.1 μg, see Figure 4 .

Embodiment 3

[0030] Same as Example 1, the difference lies in step 3), take 200 μg of the cell-like microcapsules obtained in step 2) and add it to the doxorubicin hydrochloride solution so that the concentration of doxorubicin hydrochloride is 200 μg / mL, incubate at 37°C for 12 hours; centrifuge Wash three times with phosphate buffered saline. The cancer cell-targeted cell-like microcapsule loaded with doxorubicin hydrochloride was obtained. According to the absorbance at 543nm in the ultraviolet spectrogram, the drug loading capacity of the microcapsules is 6.5 μg, see Figure 4 .

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Abstract

The invention discloses a method for preparing cancer cell targeted cell-like micro-capsules. Endothelial cells of human umbilical veins are used as raw materials for preparing cell-like micro-capsules with amino groups on surfaces, and the micro-capsules are dispersed in an aptamer solution containing aldehyde groups; after a certain period of incubation, aptamers are marked on the surfaces of the cell-like micro-capsules and then washed with a phosphate buffer solution; and the processed cell-like micro-capsules are immersed into a drug solution of doxorubicin hydrochloride, drugs are loaded through electrostatic interaction, and finally, the cell-like micro-capsules different in drug loading capacity are obtained. The preparation method is simple, controllable, wide in material source, high in production efficiency, capable of adjusting the drug loading capacity of the cell-like micro-capsules conveniently and providing the characteristics of target cancer cells simultaneously and good in application prospect.

Description

technical field [0001] The invention relates to a method for preparing cancer cell-targeted cell-like microcapsules. Especially the method of using nucleic acid aptamers to modify cell-like microcapsules to realize cancer cell targeting. Background technique [0002] The targeting of drug carriers is a very important concept. The specific recognition of the drug carrier to cancer cells can increase the concentration of the drug in the tumor tissue, so the dosage can be significantly reduced, thereby reducing the toxic and side effects on normal tissues or cells. Therefore, realizing the targeting of drug carriers is of great significance in the field of drug delivery. [0003] The usual drug carriers with targeting function mainly use artificially synthesized polymers or naturally derived proteins and polysaccharides as raw materials, and their structures are relatively simple. However, they often have poor biocompatibility, are easily recognized and cleared by the human ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/46A61K47/26A61K31/704A61P35/00
Inventor 毛峥伟张元洪
Owner ZHEJIANG UNIV
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