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Normal-temperature and low-temperature preservation diluents of milk goat seminal fluid

A technology of normal temperature preservation and low temperature preservation, applied in the biological field, can solve the problems of expanding the radiation range of elite male animal stations, the utilization rate of breeding stock, and the level of live rate cannot meet the actual production, and the conception rate cannot be guaranteed. Good liquid preservation effect and the effect of realizing the sharing of fine-grained resources

Active Publication Date: 2015-05-13
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1) Although the existing "goat semen liquid preservation method" can be stored for a long time at a viability rate of 0.3 (national standard), for dairy goats, this level of viability cannot meet the needs of actual production, and the conception rate is also low. Can not guarantee
[0006] 2) It has been reported that "a diluent for storing semen at room temperature" can keep the motility rate of semen at about 0.5-0.6, but the storage time is limited, generally no more than 24 hours. It is necessary to expand the radiation range of the fine-bred sire station and the utilization rate of breeding stock. Language cannot meet the needs of development

Method used

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  • Normal-temperature and low-temperature preservation diluents of milk goat seminal fluid
  • Normal-temperature and low-temperature preservation diluents of milk goat seminal fluid

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Dairy goat semen is prepared as diluent for storage at room temperature. The optimal content of the ingredients is: Tris-aminomethane 300mM, citric acid 94.7mM, fructose 30mM, trehalose 225mM, vitamin C5g / L, reduced glutathione 5mM, Bovine serum albumin 3g / L, adenosine triphosphate 1.2mM, 0.052g / L tylosin, 0.275g / L gentamicin, 0.17g / L lincomycin, 0.33g / L spectinomycin . After fully mixing the above ingredients with double distilled water, the dairy goat semen can be stored at room temperature. The pH value of the diluent is 6.8-7.0.

Embodiment 2

[0031] Dairy goat semen is prepared with low-temperature storage diluent, and its optimal content is: Tris-aminomethane 375mM, citric acid 124mM, fructose 40mM, trehalose 295mM, vitamin c 5g / L, reduced glutathione 5mM, bovine Serum albumin 10g / L, adenosine triphosphate 1.2mM, 0.052g / L tylosin, 0.275g / L gentamicin, 0.17g / L lincomycin, 0.33g / L spectinomycin, Fully mix the above ingredients with double distilled water to obtain mixture A, select egg yolk (egg), filter it through a 0.45 μm filter membrane, add it to the above mixture A, and mix thoroughly to obtain the dairy goat semen cryopreservation dilution. The volume fraction of egg yolk in the diluent is 20%, and the pH value of the diluent is 6.8-7.0.

[0032] Method for Preserving Fresh Essence of Dairy Goat

[0033] Fresh semen collection and quality assessment: The adult healthy ram semen was collected by the false vagina method, and the apparent motility rate, motility rate and density of the sperm were evaluated at 3...

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Abstract

The invention discloses normal-temperature and low-temperature preservation diluents of milk goat seminal fluid. Each normal-temperature and low-temperature preservation diluent comprises the following main ingredients: tris(hydroxymethyl)aminomethane, citric acid, fructose, mycose, vitamin C, bovine serum albumin, reduced glutathione, triphosadenine and compound antibiotics, and specifically, the low-temperature preservation diluent contains yolk. The normal-temperature and low-temperature preservation diluents provided by the invention can store the seminal fluid in a liquid state effectively for a short time so as to meet the artificial insemination requirement, solve the problems of short storage time limit after semen collection in a male animal station and long transportation distance between semen collection points and semen transportation points, and improve the storage quality and time effectiveness of fresh semen, thereby improving the excellent breeding animal utilization ratio and acquiring a high conception rate; meanwhile, preparation methods of the diluents are simple, the operation is simple, and the diluents are low in production cost and convenient for popularization and application on a large scale.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the diluent formula for normal temperature and low temperature preservation of dairy goat semen. Background technique [0002] As the nutritional value of goat milk is recognized and accepted by more and more people, the number of dairy goats is also increasing year by year. At present, the concentrated breeding areas in my country are mainly Shaanxi, Shandong, Henan, Hebei and Yunnan provinces, and there is a trend. A trend towards the eastern coastal areas. It is worth noting that with the popularization and popularization of artificial insemination technology in concentrated milk goat breeding areas such as Shaanxi and Shandong, artificial insemination stations for fine-bred males have gradually formed, which will help speed up the propagation of fine-bred dairy goats and improve the utilization of fine-bred males. It has played an important role in reducing production costs and i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 罗军王维席利萌孙爽高庆华张伟杨地坤朱江江史怀平
Owner NORTHWEST A & F UNIV
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