Synbiotics feed additive special for piglets and application of synbiotics feed additive
A technology of feed additives and synbiotics, applied in the field of livestock and poultry breeding, can solve the problems of lack of applicability and high efficiency, failure to consider the two-way selectivity of probiotics and oligosaccharides, and the compatibility effect cannot be maximized, so as to avoid antibiotics Residue and drug resistance, enhance digestibility and the body's anti-stress ability and immunity, improve the performance of livestock and poultry production and product quality
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Embodiment 1
[0024] Example 1 Lactobacillus plantarum ZLP001
[0025] A high-performance probiotic strain isolated from the intestinal tract of healthy piglets with acid resistance, bile salt resistance and good bacteriostasis. The survival rate of the bacteria was 90.49% in an acidic environment with pH 3.0; the survival rate in 0.3% bile salt was 87.02%; it was moderately sensitive to Escherichia coli, Salmonella and Staphylococcus aureus. It was identified as Lactobacillus plantarum (Lactobacillus plantarum) by the China Industrial Microbiology Culture Collection Management Center, and it was named ZLP001. The strain was preserved in the Microbiology Culture Collection Center on March 27, 2013 (Address: Chaoyang, Beijing No. 3, No. 1 Yard, Beichen West Road, District, Institute of Microbiology, Chinese Academy of Sciences, 100101), the classification name is Lactobacillus plantarum (Lactobacillus plantarum) ZLP001, and the preservation number is CGMCCNo.7370. Experiments show that the ...
Embodiment 2
[0026] Example 2 In vitro culture experiment
[0027] 1. Materials
[0028] Probiotics: Lactobacillus plantarum ZLP001; Oligosaccharides: mannan oligosaccharides, isomaltooligosaccharides, fructooligosaccharides, chitooligosaccharides, xylooligosaccharides, soybean oligosaccharides, raffinseed oligosaccharides, inulin.
[0029] 2. Methods and Results
[0030] 2.1 Experimental design
[0031] Add mannan oligosaccharides, isomaltooligosaccharides, fructooligosaccharides, chitooligosaccharides, xylooligosaccharides, soybean oligosaccharides, cottonseed oligosaccharides, and inulin as carbon sources to the Lactobacillus plantarum culture medium, and screen out The best oligosaccharide for growth of Lactobacillus plantarum.
[0032] 2.2 Test method
[0033] Activation: Inoculate the frozen Lactobacillus plantarum liquid into the MRS liquid medium with a 1% inoculum, and incubate at a constant temperature of 37°C for 18 hours for later use (the number of viable bacteria is equiv...
Embodiment 3
[0055] Embodiment 3 feeding test
[0056] 1. Materials
[0057] 1.1 The strain selected for probiotics and oligosaccharide tests is Lactobacillus plantarum ZLP001, which was screened and preserved by our laboratory and identified by the China Industrial Microbiology Culture Collection Management Center. It has strong acid resistance, bile salt resistance and antibacterial activity , the total number of effective viable bacteria in its freeze-dried bacterial powder preparation is 10 9 CFU / g. The oligosaccharide used in the test was fructooligosaccharide with a purity of 95%.
[0058] 1.2 Selection of animals The experimental animals were 30-day-old “Du×Long×Da” triple cross weaned piglets. The experiment was carried out in a pig farm in Fangshan District, Beijing.
[0059] 2. Methods and Results
[0060] 2.1 Experimental design and experimental diet
[0061] A total of 84 weaned piglets (30±2) days old with an average body weight of 7.19±0.45kg “Du × Chang × Da” were sele...
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