Reporter gene testing method for enhancer and promoter functions of intron SNP of CYP3A4
A CYP3A4, reporter gene technology, applied in the field of molecular biology
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[0064] Because the present invention relates to a human CYP3A4 intron sequence and a method for verifying that the SNP of the sequence has enhanced expression function, including selecting the intron sequence from the CYP3A4 genome, the conditions to be met by the CYP3A4 genome intron sequence are as follows:
[0065] 1.1 an intron located between the two exons of the CYP3A4 gene encoding the CYP3A4 enzyme, and
[0066] 1.2 The intron contains a typical GT...AG splice structure, that is, there is a GT splice site at the 5' end and an AG splice site at the 3' end, and
[0067] 1.3 Within 20 to 50 nucleotides upstream of the splicing junction at the 3′ end of the intron, there is another site that plays an important role in splicing, its sequence contains A, called the branch site, and
[0068] 1.4 In vivo experiments have confirmed that the SNP in this intron affects CYP3A4 enzyme activity, and
[0069] 1.5 The SNP variati...
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