Salmonella separation selection and detection method

Abstract

The invention discloses a salmonella separation selection and detection method which comprises the steps of enrichment, isolated culture, picking of suspected bacterial colony, biochemical test and serological identification, wherein the picking of suspected bacterial colony comprises the steps of stirring the suspected bacterial colony and picking the broken bacterial colony. According to the detection method, the salmonella is quickly identified in a large-scale salmonella screening work, so that the workload is remarkably reduced and the working efficiency is improved.

Description

technical field [0001] The invention relates to a method for separation, selection and detection of Salmonella. Background technique [0002] Salmonella is an important enteric pathogen and one of the important pathogens of food poisoning and diarrhea. [0003] At present, the detection of Salmonella mainly adopts traditional routine detection methods, molecular biology methods, immunological methods and other methods. Traditional routine detection method: Collect feces and food samples for selective enrichment and use selective separation plates (WS, SS, BS, DHL, HE, etc.) for separation. Generally, multiple suspected colonies need to be selected on the separation plate, and then tested It was identified biochemically and serologically. Using a fully automatic bacteria analyzer for detection: connect the bacteria trace biochemical identification system with a computer, the bacteria identification results are processed by the computer, and the results are automatically rep...

AI Technical Summary

Problems solved by technology

Such as enzyme-linked immunoassay (ELISA), gene chip method, polymerase chain method (PCR), Transia Salmonella card detection method, etc., these methods have better solved the specificity and sensitivity of Salmonella detection, but all require Expensive instruments and equipment, and the detection cost is high, not suitable for the screening of large samples

[0004] Although the traditional routine culture and separation technology is time-consuming and labor-intensive, it is still an indispensable and important method for disease diagnosis and hygiene evaluation, and cannot be replaced by molecular biology techniques and other tests.

However, in this method, when the suspected colonies are selected on the separation plate, the doping of a large number of suspected colonies requires subsequent biochemical and serological identification, which is time-consuming and laborious. Therefore, how to reduce the number of colonies similar to the Salmonella detected in the selected colonies is currently an issue. need to solve the problem