Bacillus cereus and probiotics powder thereof as well as preparation and application of probiotics powder

A Bacillus cereus and probiotic technology, applied in the field of microorganisms, can solve the problems of increased fermentation cost, lack of bacterial cells, limitations, etc., and achieve the effects of good safety, probiotics, good acid resistance, and good bacteriostatic activity.

Active Publication Date: 2015-04-08
河南省岳氏精忠科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN102517238A once reported a method for preparing acidogenic Bacillus cereus and bacterial preparations, but for feeding probiotic Bacillus, it also has a variety of high hydrolase activities, and has good safety and high adhesion. Not yet reported
CN102093974A once reported a strain of Bacillus cereus and its multi-stage fermentation method, but this method uses fine raw materials as the fermentation medium, which will inevitably lead to an increase in fermentation cost, and the control process is more complicated, lacking the recovery process of the thalline after the end of the fermentation liquid The link makes the preservation, transportation and use of the bacteria are greatly restricted, so this process is also difficult to promote and apply in the industrial production process of probiotics for feed

Method used

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  • Bacillus cereus and probiotics powder thereof as well as preparation and application of probiotics powder
  • Bacillus cereus and probiotics powder thereof as well as preparation and application of probiotics powder
  • Bacillus cereus and probiotics powder thereof as well as preparation and application of probiotics powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A bacillus cereus probiotic powder, its specific preparation steps are:

[0047] Step 1, slant solid culture: Bacillus cereus ( Bacillus cereus ) BC158 was inoculated on the solid slant medium, and cultured at 37°C for 24 hours to obtain the strains cultured on the slant solid, and set aside;

[0048] The composition of the solid slant medium is: by mass percentage, the solid slant medium contains 0.3% beef extract, 1% peptone, 0.5% sodium chloride, 1.5% agar powder, and the balance is water;

[0049] Step 2, primary seed culture: take 1 inoculation loop and inoculate the slanted solid cultured bacteria prepared in step 1 into a 500mL Erlenmeyer flask filled with 80 primary seed medium, and then culture it on a shaker at 34°C and 220rpm for 10h to obtain First-grade seed liquid, spare;

[0050] The composition of the primary seed medium is as follows: by mass percentage, the primary seed medium contains 0.3% yeast extract, 0.5% peptone, 0.5% sucrose, 0.3% disodium hyd...

Embodiment 2

[0059] A bacillus cereus probiotic powder, its specific preparation steps are:

[0060] Step 1, slant solid culture: Bacillus cereus ( Bacillus cereus ) BC158 was inoculated on the solid slant medium, and cultured at 37°C for 24 hours to obtain the strains cultured on the slant solid, and set aside;

[0061] The composition of the solid slant medium is: by mass percentage, the solid slant medium contains 0.4% beef extract, 1.2% peptone, 0.5% sodium chloride, 2% agar powder, and the balance is water;

[0062] Step 2, primary seed culture: take the slant solid cultured bacteria prepared in step 1 of the 3-inoculation loop and inoculate it into a 500mL Erlenmeyer flask containing 120mL primary seed medium, and then culture it on a shaker at 37°C and 250rpm for 16h to obtain First-grade seed liquid, spare;

[0063] The composition of the primary seed medium is as follows: by mass percentage, the primary seed medium contains 0.5% yeast extract, 0.7% peptone, 1.0% sucrose, 0.6% diso...

Embodiment 3

[0072] A bacillus cereus probiotic powder, its specific preparation steps are:

[0073] Step 1, slant solid culture: Bacillus cereus ( Bacillus cereus ) BC158 was inoculated on the solid slant medium, and cultured at 37°C for 24 hours to obtain the strains cultured on the slant solid, and set aside;

[0074] The composition of the solid slant medium is: by mass percentage, the solid slant medium contains 0.35% beef extract, 1.1% peptone, 0.5% sodium chloride, 1.8% agar powder, and the balance is water;

[0075] Step 2, primary seed culture: take the slant solid culture strains prepared in step 1 of the 2 inoculation loops and inoculate them into a 500mL Erlenmeyer flask filled with 100mL primary seed medium, and then culture them on a shaker at 36°C and 240rpm for 14h to obtain First-grade seed liquid, spare;

[0076] The composition of the primary seed medium is as follows: by mass percentage, the primary seed medium contains 0.4% yeast extract, 0.6% peptone, 0.8% sucrose, ...

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Abstract

A kind of Bacillus cereus and its probiotic powder and the preparation and application of the bacteria powder, the Bacillus cereus BC158 with the preservation number of CGMCC No. 7433 obtained from the feces of healthy piglets, which has a good Excellent acid resistance, bile salt resistance, high adhesion, high safety, has certain inhibitory effect on common intestinal pathogenic bacteria, and the bacteria can produce amylase, protease, cellulase; Bacillus cereus probiotics The preparation of powder adopts the four-stage cultivation process of step-by-step amplification, and the ventilation and agitation deep liquid fermentation technology realizes the high-density cultivation of Bacillus cereus BC158. high-density recovery; the preparation method is simple and low-cost, the spore rate in the prepared probiotic powder is above 95%, and the live bacteria content of Bacillus cereus is 3×109-5×1010CFU / g, which can be applied to animals Feed additives for farming.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to Bacillus cereus BC158 and its probiotic powder, as well as the preparation and application of the bacteria powder. Background technique [0002] The food safety project from the farm to the dining table is a very complex project. As a part of the food chain, feed safety is undoubtedly an important link. Therefore, it has broad prospects for strengthening the research and development and application of safe feed additives. Modern intensive animal husbandry restricts animals from contacting the soil and the surrounding natural environment. This feeding condition is not conducive to the establishment of normal flora in the gastrointestinal tract of animals. When the external environmental conditions change, it is easy to cause the imbalance of micro-ecological flora in the gastrointestinal tract of animals and reduce the resistance to pathogenic bacteria. At present, this loss is...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/085
Inventor 古绍彬吴影龚慧李市场何佳王大红张彬
Owner 河南省岳氏精忠科技有限公司
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