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Clean chitin production process with physical separation and biological enzymolysis

A technology of clean production and physical separation, applied in the field of chitin processing, can solve the problems of low purity of chitin, large amount of enzyme, uneconomical and other problems

Inactive Publication Date: 2014-01-22
YANGZHOU RIXING BIO TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method has no strong acid and strong alkali pollution, but only enzymatic deproteinization is used, the amount of enzyme required is too large, which is uneconomical, and because no strong acid and strong alkali are used, the purity of chitin obtained is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] First add 100kg of fresh shrimp and crab shells to the extrusion device, extrude and remove biomass such as protein and fat in the crab shells, and then add 0.5kg A.S1.398 Bacillus subtilis neutral protease (purchased from Wuxi Enzyme Preparation Factory, Activity 50000u / g, optimum temperature 40°C, optimum pH 7.2) and 2.5kg water diluted protease aqueous solution, enzymatic hydrolysis for 2h to 3h.

[0021] After the enzymatic hydrolysis, filter out the proteolysis solution, and the solid matter is the shrimp and crab shells after the protein has been removed.

[0022] Add 3 times the weight of aqueous hydrochloric acid solution with a concentration of 5wt% to the solid to soak for 6-8 hours, wash and drain.

[0023] Add 1.5 times the weight of 8wt% lye (can be sodium hydroxide aqueous solution or potassium hydroxide aqueous solution) to the drained solid, and heat the mixture to 90-95°C for 1.0 ~1.5 h, and then keep warm for 4~5 hours to take out the solid matter....

Embodiment 2

[0027] Add 100kg of fresh shrimp and crab shells into the extrusion device, and squeeze and remove biomass such as protein and fat in the crab shells. Dilute the protease aqueous solution formed with 0.5kg trypsin (purchased from Hangzhou Sanye Biochemical Factory, activity 4000u / g, optimum temperature 40°C, optimum pH 7.5~8) and 1.5kg water. Mix the above-extruded shrimp and crab shells with a protease aqueous solution, and enzymatically hydrolyze the protein in the extruded fresh shrimp and crab shells for 3 hours to 4 hours.

[0028] After the enzymatic hydrolysis, filter out the proteolysis solution, and the solid matter is the shrimp and crab shells after the protein has been removed.

[0029] Add 2 times the weight of aqueous hydrochloric acid solution with a concentration of 3wt% to the solid to soak for 6-8 hours, wash and drain.

[0030] Add 2 times the weight of 5wt% lye (can be sodium hydroxide aqueous solution or potassium hydroxide aqueous solution) to the dra...

Embodiment 3

[0034] Add 100kg of fresh shrimp and crab shells into the extrusion device, and squeeze and remove biomass such as protein and fat in the crab shells.

[0035] 0.35kg A.S1.398 Bacillus subtilis neutral protease (purchased from Wuxi Enzyme Preparation Factory, activity 50000u / g, optimum temperature 40°C, optimum pH 7.2) and 0.15kg trypsin (purchased from Hangzhou Sanye Biology Chemical plant, activity 4000u / g, optimal temperature 40°C, optimal pH 7.5~8) with 2~3 times of water, and then add the extruded shrimp and crab shells above, enzymatic hydrolysis time 2h~3h.

[0036] The protein in fresh shrimp and crab shells after compound enzymatic hydrolysis and extrusion, trypsin has a good effect, but the price is more expensive. It is combined with the cheaper A.S1.398 Bacillus subtilis neutral protease, and the two proteases are mixed together to enzymatically Solve the shrimp and crab shells after the above pretreatment.

[0037] After the compound enzyme enzymatic hydrolysis i...

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PUM

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Abstract

The invention discloses a clean chitin production process with physical separation and biological enzymolysis and belongs to the field of chitin processing. The chitin clean production process comprises the following steps of: with fresh shrimp and crab shells as raw material, carrying out extrusion pretreatment on the fresh shrimp and crab shells in an extruding device firstly, then carrying out enzymolysis on proteins in the shrimp and crab shells by virtue of protease, filtering out protein enzymatic hydrolysate to obtain solid substances, and carrying out the operations of calcium removal by virtue of aeration picking, closed-type degreasing by virtue of soda boiling, washing and drying on the solid substances by virtue of a closed tank to obtain a chitin product, wherein the filtered protein enzymatic hydrolysate is treated by technical processes of standing precipitation, centrifugation, drying and the like to obtain a protein product, and the protein product can be used in feed additives. With the adoption of the clean chitin production process, as the biomasses in the chitin raw materials are basically removed by virtue of a physical separation and biological extraction method before chemical treatment, and then acid-base chemical treatment is adopted to carry out the production process to comprehensively utilize the shrimp and crab shells, the acid-base consumption can be further lowered, the acid-base dosage in subsequent chemical treatment is greatly lowered, and the product yield is high.

Description

technical field [0001] The invention belongs to the field of chitin processing and relates to a chitin clean production process which can effectively reduce pollution. Background technique [0002] Chitin, also known as chitin and chitin, is another important natural polymer polysaccharide besides cellulose. The product of deacetylation of chitin is called chitosan. Chitin and chitosan are a class of renewable and biodegradable substances, which are widely used in food, chemical, pharmaceutical, agricultural and other fields. [0003] The traditional process generally uses an open-type high-concentration (10-12%) hydrochloric acid solution in a cement pool to remove calcium, phosphorus and other minerals in the crustacean shell, rinse with water until neutral; then use sodium hydroxide solution to hydrolyze the shell and the shell The protein and other components are washed with water until neutral, and finally dehydrated and dried to form chitin products. When acid-base...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 戚善龙张超柳志强从威史劲松费忠丁振中
Owner YANGZHOU RIXING BIO TECH
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