A fungus belonging to the genus Carbonara and its application in the preparation of fungi-grained wood
A technology of charcoal horn bacteria and fungi, applied in the field of microorganisms, can solve the problems of inability to process and use, excessive inhalation, incomplete wood, etc., and achieves the advantages of large-scale production and popularization and application, reasonable technical route, and operation process. simple effect
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[0062] The preparation method may also include a post-processing step, in which the hyphae on the surface of the fungal wood obtained after inoculation culture are scraped off, washed and dried to a moisture content of 8-10%.
[0063] The composition of PDA liquid medium is calculated in g / L: potato 160~240, glucose 10~20, natural pH.
[0064] The preferred components of the PDA liquid medium are: Potato 200, Glucose 15, pH 6.5~7.0 in g / L.
[0065] The wood-based material in step B is any one or more of logs, solid wood blocks, planks, veneers, wood flakes, sticks, and special-shaped wood products.
[0066] The tree species of the wood-based material in step B can be any tree species.
[0067] The tree species of the wood-based material in step B is preferably selected from the species of Populus in Salicaceae, Alnus in Betaceae, Betula, Prunus in Rosaceae, Balsa in Kapokaceae or Quercus in Fagaceae.
[0068] The tree species of the wood-based material in step B is more pref...
Embodiment 1
[0079] ——Acquisition, identification and preservation of L2Ma of Daldiniachildiae.
[0080] (1) The acquisition and identification of L2Ma of Daldiniachildiae.
[0081] The fungus belonging to the genus Carbonacola according to the present invention is isolated from decayed stumps, fallen wood or dead branches of the genus Cherry. The sample was collected from the broad-leaved forest near Jindian Park in Kunming City, Yunnan Province. A 100g sample of a decayed tree stump of the genus Cherry with black lines in the xylem was collected, together with the semicircular fungal fruiting body on it.
[0082] Cut the collected decayed tree stump samples into small pieces of 0.3~0.5cm×0.3~0.5cm×0.3~0.5cm with a scalpel, soak them in 75% alcohol for 10~15s, and then soak them in 0.1% mercuric chloride for 5 minutes , and then washed 3 times with sterile distilled water. Then place it in the enrichment medium in the dark at 26°C±2°C, and culture it statically for 10 days until the hyp...
Embodiment 2
[0094] - Preparation of fungal wood from Daldiniachildiae L2Ma (liquid method).
[0095] (1) Preparation of fungal grain wood.
[0096] The L2Ma strain Daldiniachildiae of the present invention, CGMCCNo: 5954, was inoculated on PDA liquid medium, and cultured on a dark shaker at 26°C±2°C for 9 days to form mycelium with a diameter of 0.1-3cm and the suspension of mycelium spores liquid.
[0097] The composition of PDA liquid medium is calculated in g / L: potato 200, glucose 17, pH 6.5~7.0.
[0098]Then, the Southwestern birch log to be inoculated was sawed into square pieces of 2cm×2cm×2cm (12 pieces in total), and they were placed in culture bottles with a bottom diameter of 6cm and a height of 11cm. Next, pour the vermiculite into the culture bottle to cover the wood block, add a small amount of water to moisten the wood block and vermiculite, but no excess water overflows, cover the bottle cap, and place it in a high-pressure steam sterilizer at 121 ° C. Sterilize for 40 ...
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