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Microbial preparation for marine culture and production method of microbial preparation

A technology of microbial preparation and production method, applied in the field of microbial preparation for mariculture and its production, can solve problems such as water pollution and antibiotic abuse, and achieve the effects of promoting growth, improving bottom quality, and reducing ammonia nitrogen quickly and effectively

Inactive Publication Date: 2014-01-15
DALIAN DETONG BIO TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, water pollution and serious diseases in the breeding process threaten the sustainable development of this high-efficiency industry. From 2003 to 2004, sea cucumber farming in Liaoning and Shandong provinces lost more than 2 billion yuan. Water pollution and antibiotic abuse are the primary problems to be solved.

Method used

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  • Microbial preparation for marine culture and production method of microbial preparation
  • Microbial preparation for marine culture and production method of microbial preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for producing a microbial preparation for mariculture, comprising the following steps:

[0019] 1) Use a triangular flask to hold seed culture medium no higher than 18% of the total capacity, sterilize under high pressure at 120°C for 30 minutes, and cool to room temperature;

[0020] 2) Inoculate the seawater bacillus strain preserved on the slope into the seed medium, and vibrate on a shaker at 150 rpm at 12°C for 6 hours to prepare the fermentation strain;

[0021] 3) After the fermenter is sterilized, add a fermentation medium not exceeding 65% of the total volume, sterilize at 120°C for more than 30 minutes, and cool to room temperature to prepare a liquid fermentation medium;

[0022] 4) Inoculate the fermentation strain into the liquid fermentation medium prepared in step 3), the inoculation amount is 0.1% of the volume of the liquid fermentation medium, and ferment at 28° C. for 30 hours to obtain the seawater bacillus strain used for the production pre...

Embodiment 2

[0028] A method for producing a microbial preparation for mariculture, comprising the following steps:

[0029] 1) Use a triangular flask to hold seed culture medium no higher than 18% of the total capacity, sterilize under high pressure at 120°C for 30 minutes, and cool to room temperature;

[0030] 2) Inoculate the sea bacillus strain preserved on the inclined plane into the seed medium, and vibrate on a shaking table at 150 rpm for 10 hours at 45° C. to prepare the fermented strain;

[0031] 3) After the fermenter is sterilized, add a fermentation medium not exceeding 65% of the total volume, sterilize at 120°C for more than 30 minutes, and cool to room temperature to prepare a liquid fermentation medium;

[0032] 4) Inoculate the fermentation strain into the liquid fermentation medium prepared in step 3), the inoculation amount is 0.2% of the volume of the liquid fermentation medium, and ferment at 34° C. for 30 hours to obtain the seawater bacillus strain used for the pro...

Embodiment 3

[0038] A method for producing a microbial preparation for mariculture, comprising the following steps:

[0039] 1) Use a triangular flask to hold seed culture medium no higher than 18% of the total capacity, sterilize under high pressure at 120°C for 30 minutes, and cool to room temperature;

[0040] 2) Inoculate the sea bacillus strain preserved on the inclined plane into the seed culture medium, and vibrate on a shaking table at 150 rpm for 8 hours at 25° C. to prepare the fermented strain;

[0041] 3) After the fermenter is sterilized, add a fermentation medium not exceeding 65% of the total volume, sterilize at 120°C for more than 30 minutes, and cool to room temperature to prepare a liquid fermentation medium;

[0042] 4) Inoculate the fermentation strain into the liquid fermentation medium prepared in step 3), the inoculation amount is 0.15% of the volume of the liquid fermentation medium, and ferment at 30° C. for 30 hours to obtain the sea bacillus bacteria used for th...

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Abstract

The invention relates to a microbial preparation for marine culture and a production method of the microbial preparation. The microbial preparation mainly comprises bacillus aquimaris. The microbial preparation has good characters and has the functions of well improving a substrate of a culture pond, decomposing organic wastes, reducing the content of ammonia nitrogen and nitrite and promoting growth of sea cucumbers. The bacillus aquimaris can grow in a low-temperature and high-salt environment, the acre yield of sea cucumbers can be improved in seedling growth and culture of the sea cucumbers, vibrios can be suppressed for shrimp culture, the disease rate is reduced, and the acre yield is improved. Meanwhile, the bacillus aquimaris has the characteristic of high salt resistance and can grow under the condition with the content of salt below 4%.

Description

technical field [0001] The invention relates to seawater culture technology, in particular to a microbial preparation for seawater culture and a production method thereof. Background technique [0002] In recent years, the cultivation scale of sea cucumbers in northern my country has expanded rapidly, and the output has increased significantly. In 2004, the aquaculture area and fresh sea cucumber production in Dalian were 33,000 ha and 10,000 tons[2], and in 2005 they reached 47,000 ha and 15,000 tons, respectively. In 2005, the output value of the sea cucumber breeding industry reached 1.5 billion yuan. However, water pollution and serious diseases in the breeding process threaten the sustainable development of this high-efficiency industry. From 2003 to 2004, sea cucumber farming lost more than 2 billion yuan in Liaoning and Shandong provinces. Water pollution and antibiotic abuse are the primary problems to be solved. Contents of the invention [0003] The object of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C02F103/20
Inventor 谢克焕刘英杰
Owner DALIAN DETONG BIO TECH DEV
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