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New ionizing radiation damage resistance function of polyethyleneimine compounds, and its application

A technology of polyethyleneimine and ionizing radiation, which is applied in the direction of anti-toxic agents and drug combinations, can solve the problems of high toxicity that cannot be applied, and insignificant protective effect, and achieve the effects of abundant raw materials, increasing average body weight, and reducing mortality

Inactive Publication Date: 2014-01-15
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the substances that have been reported so far to prevent and treat radiation damage have limitations, such as insignificant protective effects, high toxicity, and cannot be used in clinical practice.

Method used

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  • New ionizing radiation damage resistance function of polyethyleneimine compounds, and its application
  • New ionizing radiation damage resistance function of polyethyleneimine compounds, and its application
  • New ionizing radiation damage resistance function of polyethyleneimine compounds, and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Cytotoxicity Evaluation of PEI

[0018] 1. HEK293T cell culture

[0019] HEK293T cells were cultured in DMEM medium containing 10% fetal bovine serum and 200 μg / ml in a 37°C, 5% CO2 incubator. It was observed that the cells were in good growth state. After culturing to the logarithmic growth phase, the cells were inoculated into 96-well plates (1×10 4 Cells / well) were cultured at 37°C, 5% CO2.

[0020] 2. Preparation of PEI working solution

[0021] Branched PEI is purchased from Sigma Company (Cat.408727), light scattering method (LS) measures its weight-average molecular weight (Mw) and is about 25000, and gel permeation chromatography (GPC) records its viscosity coefficient (Mη) about 25,000. 10000, the melting point range is 73°C-75°C, the boiling point is 250°C, and the density at room temperature is 1.03g / ml. Precisely weigh 10.0mg of PEI, dissolve it in 4ml of double-antibody-containing DMEM culture solution to obtain PEI100μM stock solution; dilut...

Embodiment 2

[0026] Example 2: The activation effect of PEI on TLR5-mediated NF-κB pathway

[0027] 1. HEK293T cell culture

[0028] HEK293T cells were cultured in DMEM medium containing 10% fetal bovine serum and 200 μg / ml in a 37°C, 5% CO2 incubator. It was observed that the cells were in good growth state. After culturing to the logarithmic growth phase, the cells were seeded in 24-well plates (0.5ml / well, approximately 3×10 6 cells), CO 2 Incubate overnight in the incubator.

[0029] 2. Preparation of PEI working solution

[0030] Accurately weigh 10.0mg of PEI, dissolve it in 4ml of double-antibody-containing DMEM culture solution to obtain PEI100μM stock solution; dilute it to the working concentration with double-antibody-containing DMEM culture solution, and conduct cell experiments.

[0031] 3. Detection of activation of TLR5-mediated NF-κB pathway by dual-luciferase reporter gene assay

[0032] The NF-κB luciferase reporter gene and PEI were co-transfected into HEK293T, and ...

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Abstract

The invention relates to a new ionizing radiation damage resistance function of polyethyleneimine (PEI) compounds, and its application. The PEI mainly has two structures comprising a linear structure (the molecular formula is (C2H5N)x) and a branched structure (the molecular formula is (C2H5N)x[-N(C2H6N)C2H4-]y), wherein each of x and y is an integer not lower than 1. The PEI can specifically activate a cell TLR5 acceptor mediated NF-kappaB signal channel. Results of experiment researches show that the PEI is weakly toxic to cells and animals, can substantially activate the cell TLR5 acceptor mediated NF-kappaB signal channel and display a dosage dependency, and can substantially reduce the mortality of ionizing radiation damaged mice and prolong the animal survival time. The new ionizing radiation damage resistance function of the PEI can be used for researching medicines for preventing and treating ionizing radiation damages and damages caused by radiotherapy.

Description

Technical field: [0001] The invention relates to the new function and application of polyethyleneimine compounds in protecting against ionizing radiation damage. Background technique: [0002] With the wide application of nuclear energy and electromagnetic energy in military affairs, science and technology, and daily life, people are more and more exposed to radiation. Drugs for the prevention and treatment of radiation damage have become one of the key areas of biomedical research. Drugs for the prevention and treatment of radiation damage are one of the most effective and direct means for the treatment and protection of medical hazards caused by ionization damage. They can be used before and after exposure, but their effects are different. The most researched drugs for preventing and treating ionizing radiation damage mainly include: sulfur-containing compounds, hormones, herbal medicines, cytokines, and some new drugs based on the research on the mechanism of radiation da...

Claims

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Application Information

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IPC IPC(8): C08G73/04A61K31/785A61P39/00
Inventor 陈忠斌邢雅玲陈晓娟
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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