In-vivo tumor engineering human oophoroma tumor model established on basis of collagen aquogel

Abstract

The invention discloses application research of an in-vivo tumor engineering human oophoroma tumor model established on the basis of an injectable collagen aquogel composite human oophoroma cells in a noninvasive subcutaneous injection form to a nude mouse. The injectable collagen aquogel stent used as a substrate material is compounded with human oophoroma cell SKOV3 to noninvasively establish the in-vivo tumor engineering human oophoroma tumor model at the nude mouse subcutaneous tissues; and compared with the traditional human oophoroma tumor model established in an independent cell injection mode under the condition of no stent material, the detection on the tumor model indicates that the malignancy of the human oophoroma tumor established on the basis of the injectable collagen aquogel is higher, and is more approximate to that of the human oophoroma tumor, and the in-vivo tumor model established in such a mode has smaller trauma possibility. The tumor model established by the method has higher tumor formation rate (up to 95%), which is about 15% higher than the traditional mode, thereby providing a more reliable tumor model for further research.

Description

technical field [0001] The present invention relates to a medical tumor model—constructing an in vivo tumor engineered human ovarian cancer tumor model based on collagen hydrogel, specifically using injectable collagen hydrogel as a three-dimensional scaffold material and utilizing tumor engineering technology in nude mice Application of constructing human ovarian cancer model. Background technique [0002] The existing tumor model research is mainly based on two types, one is in vitro model, that is, the establishment of a two-dimensional tumor model; the other is the establishment of animal model, mainly a human tumor-bearing nude mouse model. [0003] As far as animal models are concerned, the traditional modeling method is to inject human tumor cells directly into the subcutaneous skin of nude mice. This method is easy to cause the loss of cells, and because there is no matrix support, the connection between cells is not tight, so the tumorigenicity is poor , There is a...

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Problems solved by technology

On the other hand, in vitro model studies have also proved that the introduction of materials provides a three-dimensional environment for tumor cells, which is closer to the in vivo environment. However, in vitro culture lacks the support of in vivo stromal cells, and the space is limited, making it difficult to reproduce the in vivo tumor microenvironment

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