Culturing medium and culturing method for producing erythrocin through fermentation of streptomyces erythreus
The technology of a fermentation medium and a culture method is applied in the field of medium for producing erythromycin by fermentation of Streptomyces erythromycin, and can solve the problems of large inoculation amount, lowering of medium quality, darkening of the color of seed liquid and fermentation liquid, and the like, Achieve the effect of reducing procurement and production costs, reducing unfavorable factors, and improving mycelial morphology
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Embodiment 1
[0087] Quality of brewer's yeast dry residue:
[0088] Protein 57.5%; Phosphorus 87.4ug / ml; Moisture 4.2%; Ash 4.1%; Carbohydrate 12.8%;
[0089] Earthworm powder quality:
[0090] Protein 63.7%; moisture 8.4%, all passed through 80 mesh sieve.
[0091] Prepare 5m 3 First-class seed liquid, including 75kg of sugarcane molasses, 25L of soybean oil, 100kg of beer yeast dry residue, 40kg of earthworm powder, 0.15kg of ammonium sulfate, 4.5kg of sodium chloride, 1kg of light calcium carbonate, and 0.05kg of silicone oil defoamer.
[0092] Prepare 20m 3 Secondary seed liquid, including 500kg of sugarcane molasses, 240L of soybean oil, 500kg of beer yeast dry residue, 220kg of earthworm powder, 1.2kg of ammonium sulfate, 18kg of sodium chloride, 8kg of light calcium carbonate, and 0.4kg of silicone oil defoamer.
[0093] Preparation 180m 3 The composition of the fermentation medium is: 3960kg of sugarcane molasses, 1800L of soybean oil, 5400kg of beer yeast dry residue, 2700kg ...
Embodiment 2
[0100] Quality of brewer's yeast dry residue:
[0101] Protein 54.3%; phosphorus 85.1ug / ml; moisture 4.6%; ash content 3.8%; carbohydrate 13.5%;
[0102] Earthworm powder quality:
[0103] Protein 62.1%; moisture 8.2%, all passed through 80 mesh sieve.
[0104] Prepare 5m 3 First-class seed liquid, including 80kg of sugarcane molasses, 30L of corn oil, 105kg of beer yeast dry residue, 45kg of earthworm powder, 0.15kg of ammonium sulfate, 5kg of sodium chloride, 1.5kg of light calcium carbonate, and 0.1kg of silicone oil defoamer.
[0105] Prepare 20m 3 Secondary seed liquid, including 520kg of sugarcane molasses, 260L of corn oil, 520kg of beer yeast dry residue, 240kg of earthworm powder, 1.4kg of ammonium sulfate, 20kg of sodium chloride, 10kg of light calcium carbonate, and 0.6kg of silicone oil defoamer.
[0106] Preparation 180m 3The composition of the above fermentation medium is: 4860kg of sugarcane molasses, 1980L of corn oil, 5760kg of beer yeast dry residue, 288...
Embodiment 3
[0113] Quality of brewer's yeast dry residue:
[0114] Protein 54.9%; phosphorus 84.8ug / ml; moisture 4.5%; ash content 3.9%; carbohydrate 13.8%;
[0115] Earthworm powder quality:
[0116] Protein 62.4%; moisture 8.5%, all passed through 80 mesh sieve.
[0117] Prepare 5m 3 First-grade seed solution, including 85kg of sugarcane molasses, 35L of rapeseed oil, 115kg of beer yeast dry residue, 50kg of earthworm powder, 0.20kg of ammonium sulfate, 5kg of sodium chloride, 1.5kg of light calcium carbonate, and 0.1kg of silicone oil defoamer.
[0118] Prepare 20m 3 Secondary seed liquid, including 540kg of sugarcane molasses, 280L of rapeseed oil, 540kg of beer yeast dry residue, 260kg of earthworm powder, 1.44kg of ammonium sulfate, 19kg of sodium chloride, 10.4kg of light calcium carbonate, and 0.61kg of silicone oil defoamer.
[0119] Preparation 180m 3 The composition of the above fermentation medium is: 5040kg of sugarcane molasses, 2340L of rapeseed oil, 5940kg of beer yea...
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