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Mortierella alpine CCFM442 strain and use thereof

A technology of Mortierella alpina and strains, applied in the fields of application, fungi, microorganisms, etc., can solve problems such as production restrictions, and achieve the effects of production safety assurance, reasonable fatty acid composition, and high use safety

Active Publication Date: 2015-05-20
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Omega-3 series PUFAs mostly come from materials such as animal liver and fish oil, and their production is limited due to factors such as environmental pollution

Method used

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  • Mortierella alpine CCFM442 strain and use thereof
  • Mortierella alpine CCFM442 strain and use thereof
  • Mortierella alpine CCFM442 strain and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: produce thalline feed additive of the present invention

[0051] The implementation steps of this embodiment are as follows:

[0052] A. Incline cultivation

[0053] The Mortierella alpina CCFM442 bacterial strain that will cryopreserve picks a loop and inoculates on the described PDA slant medium, then in the water-proof constant temperature incubator (model: GRP-9080) sold by Shanghai Senxin Experimental Instrument Co., Ltd. 14 days of constant temperature culture under the condition of temperature 25° C. to obtain the activated culture of the strain.

[0054] B. Seed cultivation

[0055] Pick a loop of the activated culture obtained in step A and inoculate it into the seed medium prepared according to the method described in the instructions, and then place it in a shaking incubator (model: ZQZY-70B) sold by Shanghai Zhichu Instrument Co., Ltd. at temperature Under the conditions of 25°C and 200rpm constant temperature shaking culture for 3 days, the...

Embodiment 2

[0067] Embodiment 2: produce thalline feed additive of the present invention

[0068] The implementation of this example is the same as that of Example 1, except that in step C, the inoculation amount of the seed solution is 5% based on the volume of the fermentation medium.

[0069] Precipitation by the method described in this specification, the moisture content of the dried thalline (thalline feed additive) prepared in this embodiment is 25.37% based on the total weight of the dry thalline, and the total fatty acid content is 9.07%, wherein the polyunsaturated fatty acid is in The content of total fatty acids is 42.37%. The ratio of omega-6 series polyunsaturated fatty acids to omega-3 series polyunsaturated fatty acids is 4.95.

Embodiment 3

[0070] Embodiment 3: produce thalline feed additive of the present invention

[0071] The implementation of this example is the same as that of Example 1, except that in step C, the inoculum amount of the seed solution is 1% based on the volume of the fermentation medium.

[0072] Precipitation by the method described in this manual, the moisture content of the dried thalline (thalline feed additive) prepared in this embodiment is 25.99% based on the total weight of the dry thalline, and the total fatty acid content is 7.64%, wherein the polyunsaturated fatty acid is in The content of total fatty acids is 43.76%. The ratio of omega-6 series polyunsaturated fatty acids to omega-3 series polyunsaturated fatty acids is 5.28.

[0073] The above test results of Examples 1, 2, and 3 are summarized, as shown in Table 2.

[0074] Table 2: Thale composition index in each embodiment

[0075]

[0076] Note: 1 The data in the table are expressed as mean ± standard deviation.

[007...

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Abstract

The invention relates to a mortierella alpine CCFM442 strain and use thereof. The mortierella alpine CCFM442 strain is used for producing thalli feed additive, the method for producing the thalli feed additive comprises the steps of slant cultivation, seed cultivation, fermentation cultivation and post treatment. The thallis feed additive is high in security. The fatty acid in the thallis feed additive is reasonable in compositions, the content of the polyunsaturated fatty acid in the total fatty acid achieves 42.4-45.8%, omega-6 / omega-3 ratio is 4.5-5.3, and meets the recommendation standard of WHO and FAO. Cheap glucose and bean pulp which are low in market price and easy to obtain, are adopted asa culture medium for producing the thallis feed additive, so that the production cost is low.

Description

【Technical field】 [0001] The invention belongs to the technical field of animal feed. More specifically, the present invention relates to a Mortierella alpina CCFM442 strain, and also relates to the use of the Mortierella alpina CCFM442 strain. 【Background technique】 [0002] Polyunsaturated fatty acids (PUFAs) can be divided into omega-6 and omega-3 series. The ω-6 series PUFAs mainly include linoleic acid (LA), γ-linolenic acid (GLA), arachidonic acid (AA) and so on. AA is an important precursor substance for human prostaglandin synthesis. It has physiological functions such as increasing blood vessel elasticity and improving immunity. It is an important nutritional supplement for milk powder. The ω-3 series PUFAs mainly include α-linolenic acid (ALA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA). EPA can reduce blood cholesterol levels and the risk of atherosclerosis. DHA plays an important role in improving the intelligence ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23K1/16C12R1/645
CPCC12N1/14C12N1/145C12R2001/645
Inventor 陈永泉陈海琴陈卫代鹏王鸿超杨芹赵建新顾震南张灏
Owner JIANGNAN UNIV
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