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Application of selenomethionine in preparation of medicine for treating Alzheimer's disease

A technology for Alzheimer's disease and selenomethionine, which is applied in drug combinations, medical preparations containing active ingredients, and pharmaceutical formulations, etc., can solve the problems of toxicity, unstable treatment effect, and low bioavailability, and achieves a reduction in Expression, improve the antioxidant capacity in the brain, reduce the effect of inflammatory response in the brain

Inactive Publication Date: 2014-01-01
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The high incidence rate and high hazard of Alzheimer's disease have brought a heavy burden to patients and society. In the prior art, sodium selenate and sodium selenite have certain therapeutic effects on Alzheimer's disease, but their The disadvantage is that it has strong toxicity in the organism, low bioavailability, and unstable therapeutic effect

Method used

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  • Application of selenomethionine in preparation of medicine for treating Alzheimer's disease
  • Application of selenomethionine in preparation of medicine for treating Alzheimer's disease
  • Application of selenomethionine in preparation of medicine for treating Alzheimer's disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0227] Example 1: Detection of the efficacy of selenomethionine at the cellular level

[0228] Selenomethionine was added to the cell culture medium at a concentration of 1uM to observe its therapeutic effect on Aβ-induced N2A cells (mouse neuroma cells). CCK-8 method was used to detect the effect of different concentrations of Se-Met on the viability of N2a cells; after N2a cells were pretreated with Se-Met, Aβ was added 1-42 (10uM) co-cultured for 6 hours; FITC-annexinⅤ / PI double-stained flow cytometry was used to detect the changes in the level of apoptosis; the DCFH-DA labeling method was used to detect the changes in the level of total reactive oxygen species (ROS) in the cells.

[0229] 1. CCK-8 detection of cell viability

[0230] CCK-8 detection found: see the results figure 1 : There is a time-related dose effect of selenomethionine on the viability of N2a cells. After 12 hours of action, with the increase of selenomethionine concentration, the cell viability is gra...

Embodiment 2

[0235] Embodiment 2: Animal level detects the efficacy of selenomethionine

[0236] 3×Tg AD mice aged 4 and 8 months were selected as drug test subjects (see Table 1), and selenomethionine (Se-Met) was added to their drinking water at a concentration of 6ug / ml for 12 weeks. After the treatment, the Morris water maze was used to detect the spatial memory and exploration ability of the mice; immunofluorescence, Western-Blot and specific kits were used to detect the changes in the following aspects: Aβ, Tau and phosphorylated proteins at each site, Inflammatory response (GFAP), pre- and post-synaptic proteins (synaptophysin and PSD95), oxidative stress levels (SOD, MDA, NO, glutathione); and detection of changes in the expression levels of BACE-1 and GSK-3β. The mechanism of its influence should be studied.

[0237] Table 1: Experimental animal grouping and treatment plan

[0238]

[0239] 1.4-month-old experimental results

[0240] 1.1 Spatial memory and exploration abilit...

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Abstract

The invention relates to an application of selenomethionine in preparation of a medicine for treating Alzheimer's disease. Specifically, selenomethionine has efficacy of improving learning and memory ability of an animal, inhibiting accumulation of Abeta in a brain and Tau protein and phosphorylation level of Tau protein, reducing GSK (glycogen synthase kinase)-3beta expression in the brain of the animal, reducing inflammatory response in the brain, reliving synaptic injury of nerve cells, improving activity of SOD (superoxide dismutase) in the brain and increasing content of glutathione with reducibility. Selenomethionine has an obvious therapeutical effect on Alzheimer's disease, and the therapeutical effect is stable; therefore, selenomethionine is a good choice for treating Alzheimer's disease clinically.

Description

technical field [0001] The invention relates to the field of drug treatment of diseases. Specifically, it relates to the use of selenomethionine for preparing medicine for treating Alzheimer's disease. Background technique [0002] The high incidence rate and high hazard of Alzheimer's disease have brought a heavy burden to patients and society. In the prior art, sodium selenate and sodium selenite have certain therapeutic effects on Alzheimer's disease, but their The disadvantage is that it has strong toxicity in vivo, low bioavailability, and unstable therapeutic effect. [0003] Therefore, there is an urgent need to find effective drugs suitable for clinical treatment. Contents of the invention [0004] The object of the present invention is to provide a medicine for treating Alzheimer's disease. [0005] To achieve the above object, the present invention provides a use of selenomethionine (Se-Met) for preparing a drug for treating Alzheimer's disease. [0006] The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/198A61P25/28
Inventor 宋国丽倪嘉缵张中豪刘琼石庆学
Owner SHENZHEN UNIV
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