Glucan beverage and preparation method thereof
A glucan and beverage technology, applied in food preparation, bacteria, lactobacillus and other directions used in food preparation, can solve the problems of narrow source, blank, lack of safety in application methods, etc., achieves low cost, simplified production process, The effect of improving innovation and safety
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preparation example Construction
[0038] Preparation of tomato juice X1, X2, X3, X4: Wash and peel the ripe tomatoes, then mechanically crush them, filter, suction filter, separate, centrifuge at 3000g for 10min, or centrifuge at 2000g for 20min to get the supernatant.
[0039] Preparation of fermentation medium S1, S2, S3 and S4: Mix sucrose and tomato juice aqueous solution according to the mass volume ratio of 3g:20mL, 1g:5mL, 1g:10mL, 1g:20mL respectively, heat until the sucrose dissolves and cool to room temperature, Na 2 CO 3 , NaHCO 3 , NaOH, and NaOH to adjust the pH to 7.0, 6.0, 7.5, and 8.0, respectively, and sterilize at 121°C for 20 minutes, 95°C for 30 minutes, 125°C for 5 minutes, and 100°C for 15 minutes. Wherein, the concentration of tomato juice in the tomato juice aqueous solution is 100%, 40%, 80%, 60% respectively, and the tomato juices are respectively tomato juice X1, X2, X3, X4.
[0040] Preparation of fermented strain (seed solution) Z1: Dissolve the freeze-dried powder of Leuconosto...
Embodiment 1
[0045] 1. Preparation of glucan beverage
[0046] (1) Add the fermented strain Leuconostoc enteroconostoc BD1710 seed solution Z1 into the fermentation medium prepared by the above method at an inoculum amount of 2.0% (v / v), the fermentation medium S1, in which sucrose and tomato The mass volume ratio of the aqueous tomato juice solution is 3g:20mL, the concentration of the aqueous tomato juice solution is 100%, the pH is 7.0, 28°C, 180rpm shaker culture for 48h, after the fermentation, the fermented liquid is taken, and after determination, the fermented liquid in the fermented liquid The dextran content was 3.22% (w / v).
[0047] (2) Completely dissolve white sugar and citric acid in water at 50°C to obtain a blending solution. The above fermented liquid and blended liquid were thoroughly mixed at a ratio of 1:5.44 (v / v), then homogenized under a pressure of 15MP, sterilized at 95°C for 30 minutes, and filled to obtain the lactic acid bacteria beverage product of this embodi...
Embodiment 2
[0061] (1) Insert the fermentation strain (seed liquid) Z2 into the fermentation medium prepared by the above method at an inoculum size of 0.5% (v / v), the fermentation medium S2, wherein the mass of sucrose and tomato juice aqueous solution The volume ratio is 4g:20mL, the concentration of the tomato juice aqueous solution is 40%, the pH is 6.0, 34°C, the fermenter is ventilated and cultured for 54h, after the fermentation, the fermented liquid is taken, and the glucan content in the fermented liquid is measured. 1.05% (w / v).
[0062] (2) Completely dissolve white sugar, citric acid, and lactic acid in water at 50°C to obtain a blending solution. The above fermented liquid and blended liquid were thoroughly mixed at a ratio of 1:1 (v / v), homogenized under 30MP pressure, sterilized at 100°C for 20 minutes, and filled to obtain the lactic acid bacteria beverage product of this example.
[0063] After testing, the content of dextran in the glucan-rich lactic acid bacteria bever...
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