Pure white new hypsizigus marmoreus bacterial strain
A technology of white stropharia and strains, applied in fungal products, botanical equipment and methods, horticulture, etc., can solve the problems of long cultivation and cultivation cycle, easy occurrence of stropharia, poor appearance quality, etc., and achieve short cultivation and Cultivation cycle, improvement of appearance quality, effect of low occurrence rate
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Embodiment 1
[0029] Embodiment 1 hybridization obtains Finc-W-62
[0030] The breeding process of the present invention's pure white mushroom strain Finc-W-62 comprises the following steps:
[0031] 1) Select parent strains: select TNN-11 and H-W as parents, the fruiting bodies of these two parents can be purchased from the market, and are also preserved in the strain collection center of Shanghai Academy of Agricultural Sciences.
[0032] 2) Preparation of culture medium
[0033] PDA medium: use 200g of potatoes, 20g of glucose and 20g of agar per 1L of medium.
[0034] Original seed culture medium: 65% of sawdust (percentage by weight, the same below), 18% of rice bran, 7% of bran, 10% of corn flour, and the water content is 60% to 62%.
[0035] Cultivated medium: 40% corn cob, 24% soybean hulls, 14% rice bran, 17% bran, 5% corn flour, and the water content is 64% to 66%.
[0036] 3) 800 heterozygotes were obtained by conventional hybridization methods.
[0037] 4) Cultivation and sc...
Embodiment 2
[0040] Embodiment 2 bacterial age test
[0041] This embodiment compares the culture period (i.e. bacterial age, the same below) and the cultivation period of the pure white shimeji mushroom strain Finc-W-62 of the present invention with the existing pure white shimeji mushroom with good quality and the most widely planted H-W, To illustrate the advantages and characteristics of the pure white mushroom strain Finc-W-62 of the present invention.
[0042] The cultivar medium used in this embodiment is: 25% corn cob, 23% sawdust, 20% soybean hulls, 12% rice bran, 15% bran, 5% corn flour, with a water content of 64% to 66%.
[0043] Table 1 Bacteria age test
[0044]
[0045] Taking H-W as a control, set 7 bacterial ages from 70d (ie days, the same below) to 100d for the culture period, and each increment of 5d is a bacterial age, and each bacterial age is 80 bottles. Cultivation and cultivation adopt conventional shimeji mushroom cultivation and cultivation parameters, the v...
Embodiment 3
[0047] Embodiment 3 parachute test
[0048] In Example 2, 10 bottles of Finc-W-62 and H-W at the 85th age in Example 2 were used as test objects, and the umbrella opening situation was recorded every day. Please refer to the results Figure 2-A , Figure 2-B , Figure 2-C And table 1~2, as can be seen from table 1, the optimal cultivation cycle of Finc-W-62 is 21d, the optimal cultivation cycle of H-W is 23d, when respectively in described optimal cultivation cycle, Finc-W-62 and The diameter of the cap of H-W is generally 1-2cm. Finc-W-62 did not open the umbrella after 5 days of continuous cultivation after the best harvest period, while H-W continued to cultivate for 1 or 2 days after the best harvest period before opening the umbrella. When harvesting, the optimal cultivation period of the two strains of Finc-W-62 and H-W is advanced 3 days, and both of them do not open umbrellas, that is, if Figure 2-A The left of the two mushroom caps shown is the 18th day of Finc-...
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