Pure white new hypsizigus marmoreus bacterial strain
A technology of white stropharia and new strains, applied in fungal products, botany equipment and methods, horticulture, etc., can solve the problems of long cultivation and cultivation cycle, prone to occurrence of Stropharia, high production cost, etc., and achieve short cultivation and Cultivation cycle, improvement of appearance quality, effect of low occurrence rate
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Embodiment 1
[0029] Example 1 Obtaining Finc-W-62 by hybridization
[0030] The breeding process of the new pure white mushroom strain Finc-W-62 of the present invention comprises the following steps:
[0031] 1) Select parent strains: select TNN-11 and H-W as parents, the fruiting bodies of these two parents can be purchased from the market, and are also preserved in the strain collection center of Shanghai Academy of Agricultural Sciences.
[0032] 2) Preparation of culture medium
[0033] PDA medium: use 200g of potatoes, 20g of glucose and 20g of agar per 1L of medium.
[0034] Original seed medium: 65% wood chips (weight percentage, the same below), 18% rice bran, 7% bran, 10% corn flour, and a water content of 60% to 62%.
[0035] Cultivated species medium: 40% corncob, 24% soybean hulls, 14% rice bran, 17% bran, 5% corn flour, and a water content of 64% to 66%.
[0036] 3) 800 heterozygotes were obtained by conventional hybridization methods.
[0037] 4) Cultivation and screenin...
Embodiment 2
[0040] Embodiment 2 bacterial age test
[0041] In this example, the culture cycle (that is, the age of the mushroom, the same below) and the cultivation cycle are compared between the new strain Finc-W-62 of the pure white mushroom of the present invention and the existing pure white mushroom of H-W, which has good quality and is the most widely planted. , to illustrate the advantages and characteristics of the new pure white mushroom strain Finc-W-62 of the present invention.
[0042] The cultivar medium used in this example is: 25% corn cob, 23% miscellaneous sawdust, 20% soybean hulls, 12% rice bran, 15% bran, 5% corn flour, and the water content is 64%-66%.
[0043] Table 1 Bacteria age test
[0044]
[0045]Taking H-W as the control, set 7 bacterial ages from 70d (that is, days, the same below) to 100d for the culture period, and each increment of 5d is a bacterial age, and each bacterial age is 80 bottles. Cultivation and cultivation adopt conventional shimeji mush...
Embodiment 3
[0047] Embodiment 3 Parachute opening test
[0048] In Example 2, 10 bottles of Finc-W-62 and H-W at the 85th age in Example 2 were used as test objects, and the umbrella opening situation was recorded every day. Please refer to the results Figure 2-A , Figure 2-B , Figure 2-C And table 1~2, as can be seen from table 1, the optimal cultivation cycle of Finc-W-62 is 21d, the optimal cultivation cycle of H-W is 23d, when respectively in described optimal cultivation cycle, Finc-W-62 and The diameter of the cap of H-W is generally 1-2cm. Finc-W-62 did not open the umbrella after 5 days of continuous cultivation after the best harvest period, while H-W continued to cultivate for 1 or 2 days after the best harvest period before opening the umbrella. When harvesting, the optimal cultivation period of the two strains of Finc-W-62 and H-W is advanced 3 days, and both of them do not open umbrellas, that is, if Figure 2-A The left of the two mushroom caps shown is the 18th day ...
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