Shinyleaf yellowhorn oil extraction method
A technology of Wenguan fruit oil and extraction method, which is applied in the direction of oil/fat production and fat production, can solve the problems of great adverse impact on human body and the environment, can only be used as animal feed, and the quality of oil is degraded, so as to improve the economic added value. , Improve the damage effect, reduce the effect of usage
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Embodiment 1
[0027] A method for extracting Xanthania sorbifolium oil, the preparation steps are as follows:
[0028] Mix the sorbifolia nuts crushed to 40 mesh and water according to the ratio of material to liquid: 1 g: 6 mL, put them in a water bath at 90 °C, stir them with magnetic force for 5 min to inactivate the enzyme, and then cool them down. At a temperature of 45 °C, a frequency of 28 kHz, and an ultrasonic wave of 250 W for 15 min; the pH of the ground slurry was adjusted to 5.8, and 0.5% pectinase and 2% cellulase were added to 2% enzyme promoter based on the sum of the mass of pectinase and cellulase. Enzymolysis in a water bath at 50°C for 6 hours; centrifuge the enzymolysis solution at 15,000 rpm for 20 minutes; after the centrifugation is completed, absorb the supernatant oil to obtain sorbifolia oil;
[0029] Wherein, the preparation method of enzyme promoter is:
[0030] (1) Dissolve 0.1 mmol of genistein in 10 mL of ethanol, and 1.5 mL of 80% hydrazine hydrate in a ro...
Embodiment 2
[0035] A method for extracting Xanthania sorbifolium oil, the preparation steps are as follows:
[0036] The sorbifolia nuts crushed to 40 mesh and water were mixed according to the material-to-liquid ratio of 1 g: 8 mL, placed in a water bath at a temperature of 90 °C, and treated with magnetic stirring for 5 min to inactivate the enzyme and then cooled. At a temperature of 45 °C, a frequency of 52 kHz, and a pretreatment of 300 W ultrasonic for 25 min; adjust the pH of the ground slurry to 5.8, add 2% pectinase and 0.5% cellulase of X. 4% enzyme accelerator based on the sum of the mass of pectinase and cellulase. Enzymolysis in a water bath at 48°C for 4 hours; centrifuge the enzymolysis solution at 18,000 rpm for 30 minutes; after the centrifugation is completed, absorb the supernatant oil to obtain sorbifolia oil;
[0037] Wherein, the preparation method of enzyme promoter is:
[0038] (1) Dissolve 0.1 mmol of genistein in 10 mL of ethanol, and 1.5 mL of 80% hydrazine hy...
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