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Method for extracting succinic acid from fermentation broth through two aqueous phase extraction

A fermentation broth and succinic acid technology, applied in the separation/purification of carboxylic acid compounds, etc., can solve the problems of low efficiency, high cost, difficulty in removing protein and glucose, etc., and achieve low energy consumption and simple operation

Inactive Publication Date: 2013-12-04
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of low efficiency, high cost, and difficult removal of protein and glucose in extracting the target product succinic acid from the succinic acid fermentation broth, and to invent a method for extracting the succinic acid in the fermentation broth through the two-phase technology. sour method

Method used

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  • Method for extracting succinic acid from fermentation broth through two aqueous phase extraction
  • Method for extracting succinic acid from fermentation broth through two aqueous phase extraction
  • Method for extracting succinic acid from fermentation broth through two aqueous phase extraction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0015] Example 1-5 Comparison of Extraction of Succinic Acid with Acid Salt and Basic Salt

[0016] Experimental conditions: take 4g of different acid salts ((NH 4 ) 2 SO 4 , NaH 2 PO 4 ) and basic salts (K 2 HPO 4 、K 3 PO 4 、K 2 CO 3 ) were dissolved in 50g / L succinic acid solution (simulated fermentation broth), and then 12g acetone was added. Shake for 3 minutes, and stand still for 8 hours to separate phases, respectively measure the volume of the upper and lower phases and the content of succinic acid, and calculate the distribution coefficient K and recovery rate Y of succinic acid; the results are shown in Table 1;

[0017] Table 1: Comparison of extraction of succinic acid with basic and acidic salts

[0018]

[0019] So choose to add acid salt.

Embodiment 6-10

[0020] The extraction situation of succinic acid in the fermented liquid under the different pH conditions of embodiment 6-10

[0021] Experimental conditions: adjust the fermented liquid of succinic acid to different pH values ​​with sulfuric acid, then get 12g fermented liquid with different pH respectively, add 4g (NH 4 ) 2 SO 4 Dissolve, then add 4g of acetone and shake for 3min, let stand for 8h to separate the phases, measure the volume of the upper and lower phases and the content of succinic acid and glucose respectively, calculate the extraction rate Y of succinic acid and the removal rate R of glucose, the results are shown in Table 2;

[0022] Table 2: Extraction of succinic acid under different pH conditions

[0023]

[0024] It can be seen that it is appropriate to choose a pH of 1~3 for the fermentation broth.

Embodiment 11-13

[0025] Examples 11-13 are different (NH 4 ) 2 SO 4 Extraction of succinic acid fermentation broth under concentration conditions

[0026] Experimental conditions: adjust the pH value of the fermentation broth to 2.0 with sulfuric acid, take 3 parts of 10g fermentation broth, add different masses of ammonium sulfate (3g, 4g, 5g) respectively, add deionized water so that the mass of each part is 15g, dissolve , then add 5g of acetone, shake for 3min, stand still for 8h to separate the phases, measure the volume of the upper and lower phases respectively, measure the content of succinic acid, glucose, protein and cells, and calculate the extraction rate Y of succinic acid and glucose, cells and protein The removal rate, the results are shown in Table 3;

[0027] Table 3 is different (NH 4 ) 2 SO 4 Extraction of succinic acid fermentation broth under concentration conditions

[0028]

[0029] It can be seen that the final concentration fraction w / w of salt is 15%~25% is mo...

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Abstract

The invention discloses a method for extracting succinic acid from fermentation broth through two aqueous phase extraction and belongs to the technical field of biochemical engineering. The method includes taking succinic acid fermentation broth produced by microbial fermentation, acidizing prior to adding acidic salt high in solubility and a hydrophilic organic solvent, forming a two aqueous phase system, separating an upper phase and performing depressurizing and distilling to recycle the organic solvent, and using activated carbon to decolor, concentrate and crystallize mother liquor to obtain succinic acid crystals; adding hydrophilic alcohol in a lower phase, respectively recycling inorganic salt and the hydrophilic alcohol. By the method, the problems of low efficiency, high cost and difficulty in removing protein and glucose in existing methods for extracting a target product succinic acid from the succinic acid fermentation broth are solved. The method has the advantages of low energy consumption, simplicity in operation and environmental protection.

Description

technical field [0001] The invention relates to a method for extracting succinic acid from a fermentation broth by using a novel two-phase system (inorganic salt / hydrophilic organic solvent) through salting-out extraction, and belongs to the technical field of biochemical industry. Background technique [0002] Succinic acid is one of the 12 most potential platform compounds identified by the US Department of Energy, and is widely used in the fields of pharmaceuticals, food and agriculture (DEPARTMENT OF ENERGY WASHINGTON DC, 2004). Fermentative production of succinic acid has made great progress in the upstream process, and the downstream process has become a bottleneck in the commercialization of fermentative production of succinic acid. In the process of fermentative production of succinic acid, downstream extraction and purification processes account for more than 60% of the cost (Chemical engineering & technology, 2008, 31(5): 647-654). At present, for the extraction o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C55/10C07C51/42C07C51/48
Inventor 郑璞古博华
Owner JIANGNAN UNIV
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