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Tissue culture and rapid propagation method of poinsettia

A tissue culture, Christmas red technology, applied in the field of plant tissue culture, can solve the problems of not yet forming a mature culture system, difficulty in rooting seedlings, and little research on Christmas red, and achieves strong ability to absorb water and nutrients, low cost, and low cost. short cycle effect

Inactive Publication Date: 2013-11-27
张曦予 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are not many studies on tissue culture and rapid propagation of Christmas red in China, and a mature culture system has not yet been formed, especially in the rooting of seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] Select a healthy, clean potted Christmas red small plant free of diseases and insect pests; use sterilized scissors to cut off the tender top bud and 2 side buds (with leaves) on the upper part of the plant, add a small amount of detergent, and rinse with tap water for 5 minutes; Put it into a sterile beaker, on the ultra-clean table, first soak it with sterile water for 4 times (30S each time); then soak it in 75% alcohol for 60S, discard the alcohol; then wash it with 0.1% HgCl 2 The solution was sterilized for 10 minutes, and the waste liquid was discarded; finally, it was washed 7 times with sterile water, and the material was transferred to a sterile petri dish with absorbent paper, and the water was blotted;

[0022] Inoculate the above-mentioned sterilized stem explants with terminal buds or lateral buds in the primary medium (MS+6-BA1.0mg / L+NAA 0.1mg / L+0.6% agar+3% sucrose, pH value 5.8 ), the culture temperature is 22°C, the light intensity is 2000Lx, and the l...

example 2

[0027] Select a small potted Christmas red plant that is free from diseases and insect pests, grows robustly, and is clean; use sterilized scissors to cut off the tender top buds and 3 side buds (with leaves) on the upper part of the plant, add a small amount of detergent, and rinse with tap water for 3 minutes; Put it into a sterile beaker, and soak it in sterile water for 5 times (60S each time) on the ultra-clean table; then soak it in 75% alcohol for 50S, discard the alcohol; then use 0.1% HgCl 2 The solution was sterilized for 9 minutes, and the waste liquid was discarded; finally, it was washed 5 times with sterile water, and the material was transferred to a sterile petri dish with absorbent paper, and the water was blotted;

[0028] Inoculate the above-mentioned sterilized stem explants with terminal buds or lateral buds in the primary culture medium (MS+6-BA1.5mg / L+NAA 0.15mg / L+0.6% agar+3% sucrose, pH value 6.0 ), the culture temperature was 24°C, the light intensity...

example 3

[0033] Select a healthy, clean potted Christmas red small plant free of diseases and insect pests; use sterilized scissors to cut off the tender top buds and 5 side buds (with leaves) on the upper part of the plant, add a small amount of detergent, and rinse with tap water for 4 minutes; Put it into a sterile beaker, and soak it in sterile water for 4 times (45S each time) on the ultra-clean table; then soak it in 75% alcohol for 55S, discard the alcohol; then wash it with 0.1% HgCl 2The solution was sterilized for 8 minutes, and the waste liquid was discarded; finally, it was washed 6 times with sterile water, and the material was transferred to a sterile petri dish with absorbent paper, and the water was blotted;

[0034] The above-mentioned sterilized stem explants with terminal buds or lateral buds were inoculated in the primary medium (MS+6-BA1.2mg / L+NAA 0.13mg / L+0.6% agar+3% sucrose, pH value 5.8 ), the culture temperature was 23°C, the light intensity was 2000Lx, and th...

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Abstract

The invention relates to a tissue culture and rapid propagation method of poinsettia, which belongs to the technical field of plant tissue culture. The tissue culture and rapid propagation method is characterized by comprising the steps of rapidly forming a large number of proliferation seedlings on a sterile culture medium by utilizing terminal buds or lateral buds of potted poinsettia plantlets, transplanting the seedlings into an outdoor small flowerpot after being induced to root, growing for 8-9 months and selling as a commodity, wherein the survival rate is high (above 89%). In the production of poinsettia, cuttage is mainly used for propagation, but as branches contain rich latex, the cuttage survival rate is low; the tissue culture and rapid propagation are high in difficulty of seedling rooting and low in rooting rate. Compared with conventional cuttage propagation, the tissue culture and rapid propagation method is high in efficiency, potted plants are good in shape, strong in growth vigor and high in commodity value, the rooting rate of tissue culture seedlings is high (reaching 80-85%, and overcoming the problem that the seedlings are difficult to take root), the culture cycle is appropriate (taking about 80 days from explant buds to rooted seedlings), the proliferation multiple is high (being 8-10 times), and the hormone combination of the culture medium is simple (being low in cost and convenient to promote).

Description

technical field [0001] The invention relates to a tissue culture and rapid propagation method of ornamental flower Christmas red, which belongs to the field of biotechnology, and specifically belongs to the category of plant tissue culture. Background technique [0002] Christmas red( Euphorbia pulcherrima) , also known as poinsettia, is an evergreen or semi-evergreen shrub of the genus Euphorbia in the Euphorbiaceae family. When it blooms, its leaves are vermilion and very beautiful, mainly for viewing. It is native to Central America and Mexico, and is now cultivated in various places in my country. Its ornamental value and economic value are high. [0003] During the period of New Year's Day and Spring Festival, most flowers and trees are still in dormant stage, Christmas red is covered with green leaves, and the top of the plant is supported by bright red petal-shaped bracts, which are large in shape and bright in color, very eye-catching, and are excellent flowers fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张曦予李宗菊冯辽辽赵昱胡静马雪艳
Owner 张曦予
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