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Method for producing beautiful millettia root polysaccharides from non-embryonic cells of millettia dielsiana through suspension cultivation

A kind of beautiful chicken blood vine, cell suspension technology, applied in biochemical equipment and methods, plant cells, tissue culture and other directions, can solve problems such as the difficulty of guaranteeing the yield and quality of polysaccharide in cattle, the impact of yield and quality, and the occupation of large arable land. Achieve the effect of large development and utilization value, consistent growth cycle, and no occupation of cultivated land

Active Publication Date: 2015-03-25
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the former has led to the near depletion of the resources of the wild beautiful thuja vine, while the latter adopts the method of artificial planting. Due to the long growth cycle of the plant, it needs to occupy a large amount of cultivated land for a long time, and the yield and quality are affected by natural conditions and artificial cultivation techniques. However, the yield and quality of Niu Dali polysaccharide are difficult to guarantee

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  • Method for producing beautiful millettia root polysaccharides from non-embryonic cells of millettia dielsiana through suspension cultivation
  • Method for producing beautiful millettia root polysaccharides from non-embryonic cells of millettia dielsiana through suspension cultivation
  • Method for producing beautiful millettia root polysaccharides from non-embryonic cells of millettia dielsiana through suspension cultivation

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Acquisition of non-embryogenic single-cell suspension cell line of Spathifolia and production of crude polysaccharide from Spathiphyllum non-embryogenic single-cell suspension culture cells

[0042] (1) Acquisition of the non-embryogenic single-cell suspension cell line of Spathiphyllum Spatholobus

[0043] (1) Take the young stems of Spatholobus sagittatus as explants. The young stems are 1-2cm in length and contain 1-2 axillary buds. base + sucrose 30g / L+6-BA3mg / L+NAA0.5mg / L+0.8g / L agar, pH5.85), cultured at 27℃, with a light intensity of 3000Lux and a daily light duration of 12 hours. After 30 days, non- Embryogenic callus, the diameter of non-embryogenic callus is about 0.5cm;

[0044] (2) Inoculate the callus with a diameter of about 0.5cm (non-embryogenic callus is 0.125-0.25cm) 3 ,) non-embryogenic callus was transferred to callus proliferation medium (MS medium+sucrose 30g / L+6-BA4mg / L+Picloram0.5mg / L+0.8g / L agar, pH5.85), 27 Cultivate in dark at ℃, and the cu...

Embodiment 2

[0051] Identification of the main chemical constituents of the suspension culture cells of Spatholobus palisana

[0052] (1) TLC identification: take 1L of suspension cells of Spatholobus palisana and 100g of dried bovine herb (as a control), add distilled water to 1L, heat at 100°C for 1 hour, filter and save the extract, add 1L of distilled water to the residue, and repeat The above steps were performed once, and the extracts were combined. Take 5 μL of the test solution of Spatholobus palisana and the extract of Niu Dali, and load the sample on the same 5cmх4cm Merck SG60F 245 On the prefabricated plate, develop with chloroform-methanol (volume ratio 6:1), use 5-10% concentrated sulfuric acid ethanol solution as color developer, and heat to develop color. The results showed that the bands of the test sample and the extract (standard) obtained from Herba Dali herb were consistent ( figure 2 ), indicating that the ingredients of the two are the same, and the main ingredien...

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Abstract

The invention discloses a method for producing beautiful millettia root polysaccharides from non-embryonic cells of millettia dielsiana through suspension cultivation. The method comprises the following steps of: taking the explant of millettia dielsiana and cultivating the explant on a callus induction culture medium, thereby obtaining non-embryogenic callus; carrying out multiplication culture of the non-embryogenic callus on a callus multiplication culture medium, thereby obtaining a rapidly grown, loose cell aggregate; inoculating the cell aggregate to a cell suspension starting culture medium for cultivation, thereby obtaining a liquid cultured single cell suspension system; after centrifugation of the single cell suspension system, inoculating the precipitate to a liquid multiplication culture medium, thereby obtaining suspension cells; extracting the suspension cells with water and then adding absoluteethylalcohol to the extract until the volume percent content of ethanol in the solution is 60-75%, and carrying out rotary evaporation on the supernate, thereby obtaining the coarse beautiful millettia root polysaccharides. The method provided by the invention has the characteristics of short production period, no occupation of cultivated land, stable yield, high active ingredient content and no damage on natural resources, and therefore, the method has great development and utilization value.

Description

technical field [0001] The invention relates to a method for producing bovine herbaceous polysaccharides by suspension culture of non-embryogenic cells of Spatholobus palismanthus. Background technique [0002] Spathiphyllum is a perennial vine-like shrub of the Fabaceae Papilionaceae subfamily, which is distributed in tropical and subtropical regions. Its swollen root (medicinal material name: Niu Dali) is a rare and precious wild medicine. Niu Dali was first recorded in "The Essentials of Raw Herbal Medicine", and it is often recorded in modern herbal medicines, and it is the main raw material of many kinds of Chinese patent medicines. Niu Dali has the effects of nourishing the lungs and kidneys, clearing away heat and relieving cough, relaxing tendons and activating collaterals, etc. In addition, Niu Dali is widely used in Guangdong, Guangxi and Hainan as raw materials for soup, medicinal diet, and medicinal wine. It has the effect of regulating the functions of the lun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C12N5/04C12N5/02
Inventor 乔飞徐立王荣香江雪飞李志英赵小青丛汉卿王茂媛李丽黄碧兰李克烈
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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