Feeding attractant for piglets and production method thereof
A production method and technology of attractant, applied in application, animal feed, animal feed, etc., to achieve the effects of simplifying control means, promoting appetite, and increasing concentration
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Embodiment 1
[0050] This example describes the culture method of the microorganisms used in the present invention.
[0051] 1. Cultivation of Lactobacillus delbrueckii subspecies Bulgaria:
[0052] 1-1. Activation of strains: Use an inoculation needle to take out a ring of strains and insert it into 5mL activation medium. The composition of the activation medium is: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast extract, 5.0g glucose, Sodium acetate 5.0g, diamine citrate 2.0g, Tween 80 1.0g, K 2 HPO 4 2.0g, MgSO 4 .7H 2 O 0.2g, MnSO 4 .H 2 O 0.05g, CaCO 3 20.0g, 15.0g agar, 1.0L distilled water, pH 6.8. Sterilize at 121°C for 20 minutes; place in a biochemical incubator at 43°C for static incubation for 16 hours.
[0053] 1-2. Strain cultivation for fermentation: Take 5mL of the activated strain and insert it into 100mL of seed medium. The composition of the seed medium: 30g of soybean meal, 10g of glucose, 1.0L of distilled water, natural pH, sterilized at 115°C 20 minutes...
Embodiment 2
[0058] This example describes a preferred production method of the attractant.
[0059] After mixing 50 parts by weight of soybean meal, 30 parts by weight of glucose, and 20 parts by weight of milk powder, add warm water at 38°C to make the water content of the mixture 40%, add 10% (v%, refers to solids (soybean meal, Glucose and milk powder): Seed liquid = w: v%) Saccharomyces cerevisiae cultured in step 2, incubated for 16 hours, and inserted into 30% (v%) of lactic acid bacteria cultured in step 1, referring to solid matter (soybean meal, Glucose and milk powder): seed liquid = w: v%), incubated at 43°C for 60 hours. After the cultivation is over, the material is dried with a vibrating fluidized bed, and the water evaporated during the drying process is treated with a tubular cooler (the design standard of the cooler: the inlet temperature is 115°C, and the outlet temperature is adjustable from 50-90°C) For cooling, collect the cooling liquid cooled by the cooler, filter ...
Embodiment 3
[0063] This example describes a preferred production method of the attractant.
[0064] After mixing 80 parts by weight of soybean meal, 10 parts by weight of glucose, and 10 parts by weight of milk powder, add warm water at 38°C to make the water content of the mixture 60%, add 10% (v%, refers to solids (soybean meal, Glucose and milk powder): Seed liquid = w: v%) Saccharomyces cerevisiae cultivated in step 2, incubated for 16 hours, and inserted into 2% (v%) of lactic acid bacteria cultivated in step 1, referring to solid matter (soybean meal, Glucose and milk powder): seed liquid = w: v%), incubated at 43°C for 60 hours. After the cultivation is over, the material is dried with a vibrating fluidized bed, and the water evaporated during the drying process is treated with a tubular cooler (the design standard of the cooler: the inlet temperature is 115°C, and the outlet temperature is adjustable from 50-90°C) Cooling, collect the cooling liquid cooled by the cooler, filter a...
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