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A method for producing high-efficiency protein feed with feed enzymes cooperating with Bacillus coagulans

A technology of Bacillus coagulans and protein feed, which is applied in the field of biotechnology fermentation to achieve the effects of reducing production costs, improving health, and improving the body's immunity and disease resistance

Active Publication Date: 2021-02-19
南宁精成生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few research reports on improving the nutritional value and utilization rate of vegetable protein (mainly soybean meal) by utilizing the synergistic effect of bacteria and enzymes.
The method of cooperating with Bacillus coagulans to produce high-efficiency protein feed with feed enzymes has not been reported so far

Method used

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  • A method for producing high-efficiency protein feed with feed enzymes cooperating with Bacillus coagulans
  • A method for producing high-efficiency protein feed with feed enzymes cooperating with Bacillus coagulans
  • A method for producing high-efficiency protein feed with feed enzymes cooperating with Bacillus coagulans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Implement as follows: the following components are calculated by mass percentage

[0045] (1) Preparation of Bacillus coagulans seed solution from different sources: pick one ring of Bacillus coagulans slant, inoculate it into a 250mL Erlenmeyer flask filled with 100mL LB liquid medium, cultivate it on a shaking table at 37°C, 180r / min for 6h, and make seeds liquid;

[0046] (2) Bacillus coagulans liquid culture: medium formula: yeast powder: 15g / L, sodium chloride: 5g / L, maltose: 8g / L, starch: 15g / L, magnesium sulfate: 0.8g / L, manganese sulfate : 0.2g / L, disodium hydrogen phosphate: 1g / L, sodium dihydrogen phosphate: 3g / L, initial pH value: 7.4. When cultivating, the liquid volume of the fermentation tank is 40%, the volume of seed liquid is 3%, the rotation speed is 160r / min, and the ventilation volume is 0.6m 3 / h, cultivated at 35°C for 18h;

[0047] (3) Solid-state culture of Bacillus coagulans: material (soybean meal: bran = 1:9) and the bacterial solution obta...

Embodiment 2

[0059] Implement as follows: the following components are calculated by mass percentage

[0060] (1) Preparation of Bacillus coagulans (Bacillus coagulans GIM1.646) seed solution: pick 1 ring of Bacillus coagulans slant, inoculate it into a 250mL Erlenmeyer flask containing 100mL LB liquid medium, shake the table at 37°C, 180r / min Cultivate for 6 hours to make seed solution;

[0061] (2) Bacillus coagulans liquid culture: medium formula: yeast powder: 30g / L, peptone: 10g / L, sodium chloride: 1g / L, disodium hydrogen phosphate: 5g / L, maltose, starch, magnesium sulfate, Manganese sulfate content design test (see Table 2), initial pH value: 6.8. When cultivating, the liquid volume of the fermentation tank is 20%, the seed liquid volume is 2%, the rotation speed is 200r / min, and the ventilation volume is 0.6m 3 / h, cultivated at 39°C for 20h;

[0062] Table 2 Design experiment of content of maltose, starch, magnesium sulfate and manganese sulfate

[0063] Test No. A M...

Embodiment 3

[0074] Implement as follows: the following components are calculated by mass percentage

[0075] (1) Preparation of Bacillus coagulans (Bacillus coagulans GIM1.646) seed solution: pick 1 ring of Bacillus coagulans slant, inoculate it into a 250mL Erlenmeyer flask containing 100mL LB liquid medium, shake the table at 37°C, 180r / min Cultivate for 6 hours to make seed solution;

[0076] (2) Bacillus coagulans liquid culture: medium formula: yeast powder: 25g / L, peptone: 5g / L, sodium chloride: 2g / L, maltose: 6g / L, starch: 20g / L, magnesium sulfate: 1.2 g / L, manganese sulfate: 0.3g / L, disodium hydrogen phosphate: 3g / L, sodium dihydrogen phosphate: 5g / L, initial pH value: 7.2. When cultivating, the liquid volume of the fermentation tank is 60%, the seed liquid volume is 1%, the rotation speed is 120r / min, and the ventilation volume is 0.6m 3 / h, cultivated at 37°C for 15h;

[0077] (3) Solid-state culture of Bacillus coagulans: material (soybean meal: bran = 1:9) and the bacterial...

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Abstract

The invention discloses a method for producing high-efficiency protein feed by synergizing bacillus coagulans with feed enzymes, and belongs to the field of biotechnology fermentation. The present invention utilizes bacterial enzymes to synergistically process the soybean meal, does not need to sterilize the soybean meal, has facultative anaerobic and anaerobic fermentation in the whole process, and has low energy consumption and low dry matter loss. The technological process of the invention is simple and convenient to operate, and is suitable for both direct production and feeding in farms and large-scale production in feed mills, and the cost of raw materials is low, so it is suitable for industrialized production. The fermented soybean meal of the invention has strong sour flavor and strong palatability. The pH is between 3.2 and 4.5, and the organic acid content is above 2.8%. The acid-soluble protein is more than 6.5 times higher than that before fermentation, and the relative molecular weight of soluble protein is within 500, accounting for more than 60%. The urease activity is lower than 0.073U / g, and the raffinose content is low. At 0.42%, stachyose is less than 0.035%; the number of spores of lactic acid bacteria coagulans reaches 2.0×10 9 More than cfu / g.

Description

technical field [0001] The invention belongs to the field of biotechnology fermentation, and in particular relates to a method for producing high-efficiency protein feed by cooperating with bacillus coagulans with feed enzymes. Background technique [0002] The absorption of macromolecular proteins by animals is obviously not as good as the absorption of small molecular polypeptides and amino acids. At the same time, there are many anti-nutritional factors in plant protein raw materials, such as urease, trypsin inhibitor, phytic acid, lectin, lipoxygenase and Soybean antigenic protein, etc. affect the digestion, absorption and metabolism of nutrients, and have adverse effects on the health and production performance of animals, thus restricting the more effective application of plant protein in poultry breeding. [0003] The current methods for producing high-efficiency biological protein feed mainly include protease hydrolysis and microbial degradation. [0004] The use of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/18A23K10/14A23K10/37
CPCY02P60/87
Inventor 姚勇芳廖延智吴少微何秀婷谭才邓宋雪果魏强华朱美娟司徒满泉李平凡
Owner 南宁精成生物科技有限公司
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