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Kit for detecting genetic risk of multiple myeloma

A technology for multiple myeloma and genetic risk, applied in kits for detecting genetic risk of multiple myeloma, in the fields of medicine and biology, can solve problems such as the decline of the body's ability to detoxify

Inactive Publication Date: 2013-10-30
解码(上海)生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This may be because the deletion of the GSTT1 gene will reduce the detoxification ability of the body, and the accumulation of carcinogens in the body will greatly increase the risk of various cancers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1. Use of detection kits

[0023] 1. Extract DNA template

[0024] The epithelial cells of the oral mucosa of the subjects were scraped, and the genomic DNA was extracted by the phenol-chloroform method.

[0025] 2. PCR amplification reaction

[0026] Use the PCR reaction component in the detection kit, which contains the following primer pairs:

[0027] (1) MTHFR (C677T) forward primer: 5'AGGGAGGCTTCAACTACGC3'

[0028] MTHFR (C677T) reverse primer: 5'GCGGTTGAGGGTGTAGAAGT3'

[0029] (2) MTHFR (A1298C) forward primer: 5'GCCTCCAGACCAAAGAGTTACAT3'

[0030] MTHFR (A1298C) reverse primer: 5'CCACTCCAGCATCACTCACTTT3'

[0031] (3) GSTT1 (Null / Present) forward primer: 5'GAACTCCCTGAAAAGCTAAAGC 3'

[0032] GSTT1 (Null / Present) reverse primer: 5'GTTGGGCTCAAATACGGTGG3'

[0033] The reaction system for PCR amplification is: 10×PCR reaction buffer 2.5ml; 25mM dNTP mixture 0.2ml, 5U / ul Taq enzyme 0.125ml, DNA template 1ml (about 12-15ng), 20uM forward primer and reverse ...

Embodiment 2

[0048] Example 2. Services for people to be tested for genetic risk of susceptibility to the onset of multiple myeloma

[0049] 1. Sampling and DNA extraction

[0050]The physicians in the laboratory department of the hospital instructed the subjects to use oral swabs to sample oral epithelial cells, and the phenol-chloroform method was used to extract DNA from oral epithelial cells.

[0051] 2. Genotype detection

[0052] Using the kit provided by the present invention, the polymorphisms of the C677T site and the A1298C site on the MTHFR gene of the subject's genomic DNA, and the three single nucleotides of the GSTT1 gene deletion (Present / Null) site polymorphism The acid polymorphic loci were subjected to DNA sequencing to determine the genotypes of the three SNPs loci.

[0053] 3. Risk assessment of multiple myeloma high-risk groups

[0054] Through the analysis of the SNPs genotypes of the subjects, a multiple myeloma susceptibility gene risk assessment and analysis rep...

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PUM

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Abstract

The invention provides a kit for detecting genetic risk of multiple myeloma. The kit comprises a specific primer which detects genotypes of three single nucleotide polymorphic sites, polymorphism of C677T site and A1298C site on a MTHFR (Methylene Tetrahydrofolate Reductase) gene and polymorphism of sites with or without GSTT1 (Glutathione S-transferase Theta 1) gene (Present / Null) as well as a DNA (Deoxyribonucleic Acid) sequencing primer, a PCR (Polymerase Chain Reaction) reaction assembly, a PCR product purifying assembly, a DNA sequencing reaction assembly and the like. The kit provided by the invention estimates the risk level of the subject suffering from multiple myeloma by detecting the genotypes of three single nucleotide polymorphic sites closely associated with multiple myeloma, and finally guides the subject to prevent occurrence of multiple myeloma in a targeted manner from genetic layer according to the gene detection result of each subject, so that the morbidity rate of multiple myeloma is reduced.

Description

technical field [0001] The invention belongs to the field of molecular biology, relates to medicine and biotechnology, and specifically relates to a kit for detecting the genetic risk of multiple myeloma. Direction guide for multiple myeloma. Background technique [0002] Multiple myeloma (multiple myeloma, MM) is a malignant tumor with abnormal proliferation of plasma cells. A progressive neoplastic disease. It is characterized by myeloid plasmacytoma and hyperproliferation of an intact monoclonal immunoglobulin (IgG, IgD, or IgE) or Bence Jones protein. Multiple myeloma is often accompanied by multiple osteolytic lesions, hypercalcemia, anemia, kidney damage, and increased susceptibility to bacterial infection, normal immunoglobulin production is suppressed. The incidence rate is estimated to be 2-3 / 100,000, the male to female ratio is 1.6:1, and most patients are >40 years old. [0003] The full name of MTHFR is 5,10-methylenetetrahydrofolate reductase encoding gen...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 潘加奎姜丽
Owner 解码(上海)生物医药科技有限公司
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