Genetic testing method for menopause medication instruction

A technology for medication guidance and subjects, applied in the fields of molecular biology and medicine, can solve problems such as induction and inability to relieve menopausal hot flashes and aggravation

Inactive Publication Date: 2011-06-29
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Raloxifene is one of the important members. It has estrogen-like effects on lipoproteins in bone tissue and serum. It has been used to treat osteoporosis in postmenopausal women. It can protect the cardiovascular system and improve cognitive function. SERMs cannot relieve symptoms such as menopausal hot flashes and sweating, and may even induce or aggravate these symptoms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0008] Step 1: Extraction of DNA template

[0009] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0010] Step 2: Fluorescent Quantitative PGR Reaction

[0011] Use the fluorescent quantitative PCR kit in the detection kit to carry out the fluorescent quantitative PCR reaction. The reaction system has a total volume of 10 μl, including 2 μl of DNA template with a concentration of 20 ng / μl, 1 μl of 10X fluorescent quantitative PCR reaction buffer, and 0.1 μl of 25 mM dNTP mixture solution, 0.6μl 25mM MgCl 2 solution, 0.025 μl (5 units / μl) Taq DNA polymerase, 0.225 μl each of 20 μM sense primer and antisense primer, 0.25 μl each of 10 μM fluorescent probe with VIC and fluorescent probe with FAM, and 5.325 μl of deionized water.

[0012] The reaction was carried out on a PCR amplification instrument, and the reaction conditions were 50° C. for 2 minutes, 95° C. for 10 minutes, and 60 cycles of 95° C. for 30 seconds and 60° C. for 1 minute. After ...

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PUM

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Abstract

The invention discloses a genetic testing method for menopause medication instruction, comprising the following steps: collecting oral mucosa cells of a subject, extracting the genome DNA, and testing the respective polymorphism of the rs4646903 site on the CYP1A1 gene of the genome DNA, the Leu432Val site on the CYP1B1 gene of the genome DNA, the Val158Met site on the COMT gene of the genome DNA and the Present / Null site on the GSTT1 gene of the genome DNA, thereby evaluating the menopause medication metabolic capability on a gene level and using the medication metabolic capability as the medication instruction.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine, and more specifically, the present invention relates to a method for menopausal medication guidance genetic detection. Background technique [0002] Entering perimenopause is a special stage for women, which is affected by biological, psychological and social factors. Modern medical research believes that ovarian function decline, sex hormone secretion decrease, gonadotropin increase, and inadaptation to the readjustment of the body's endocrine cause vegetative Nervous dysfunction is the main cause of menopausal syndrome. Scientific research has found that estrogen receptors are widely distributed on the cell membranes of women's tissues and organs throughout the body. When estrogen decreases, these tissues and organs will undergo degeneration or metabolic changes, and then a series of neurological, endocrine, Metabolic and psychological imbalances cause a syndrome of chang...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 冯哲民邹祖烨贺宪民
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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