Noninvasive lung adenocarcinoma susceptibility gene assay kit

Abstract

The invention provides a noninvasive lung adenocarcinoma susceptibility gene assay kit. The kit comprises specific primers and DNA (deoxyribonucleic acid) sequencing primers for assaying the genotypes at the lLe462Val and MSPI polymorphic loci on CYP1A1 gene, the Arg399G1n polymorphic locus on XRCC1 gene, the C677T polymorphic locus on MTHFR gene, the Present / Null polymorphic locus on GSTM1 gene and the Present / Null polymorphic locus on GSTT1 gene as six single-nucleotide polymorphic loci, a PCR (Polymerase Chain Reaction) reaction component, a PCR product purification component, a DNA sequencing reaction component and the like. By assaying the genotypes at the six single-nucleotide polymorphic loci closely related to the incidence of lung adenocarcinoma, the kit can evaluate the risk level of lung adenocarcinoma affecting an examinee and finally direct the targeted prevention of the incidence of lung adenocarcinoma at the genetic level according to the genetic testing result of each examinee in order to decrease the incidence of lung adenocarcinoma. The sampling method of the kit samples oral mucosa cells, and therefore is painless and noninvasive and prevents cross infection. The sequencing assay result is accurate and reliable, expensive imported special instruments do not need to be purchased, and the method is easy to popularize.

Description

Technical field [0001] The invention belongs to the field of molecular biology, and relates to medicine and biotechnology, and specifically relates to a lung adenocarcinoma susceptibility gene detection kit, which evaluates the risk level of lung adenocarcinoma from the genetic level according to the test results, and serves as the prevention and treatment of lung glands Directional guidance for cancer. Background technique [0002] Lung adenocarcinoma accounts for about 20%-30% of all types of lung cancer. Lung adenocarcinomas mostly originate from epithelial cells that secrete mucus in the smaller bronchial mucosa. Therefore, most adenocarcinomas are located in the peripheral part of the lung and are spherical masses near the pleura. Female patients are more common, and the age of onset is also younger. Lung adenocarcinoma is not closely related to smoking. In some cases, the cancer occurs on the basis of lung fibrous scar lesions. Adenocarcinoma generally has no obvious cli...

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Problems solved by technology

Molecular biological research also shows that the entire gene of GSTT1-deficient individuals is deleted and loses its function, which is equivalent to increasing the concentration of environmental toxins and carcinogens, and increasing the individual's susceptibility to a series of types of cancer.

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