Composition and method for restraining growth of oral pathogenic bacteria

A technology of composition and pathogenic bacteria, applied in oral care, drug combination, food preparation, etc., can solve the problems of reducing the effect of commodity development, difficult preservation of lactobacillus, etc., to achieve inhibition of growth and adhesion, difficult activity, and antibacterial The effect of good ability

Inactive Publication Date: 2013-10-23
林秀霞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The concept of using probiotics to adjust the bacterial phase of oral microorganisms to achieve the purpose of oral health has been practiced in academia for many years, and many clinical trials have shown the feasibility of this method, most of which are Lactobacillus spp. .), and some strains have been clinically proven to reduce dental caries or inhibit the growth of Streptococcus saccharis. Among them, Lactobacillus salivarius has a certain effect on preventing periodontal disease and reducing bad breath, but Lactobacillus It is not easy to preserve itself, so it may reduce the original effect or limit the development of its products

Method used

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  • Composition and method for restraining growth of oral pathogenic bacteria
  • Composition and method for restraining growth of oral pathogenic bacteria
  • Composition and method for restraining growth of oral pathogenic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Counting the number of probiotic bacteria of A brand oral tablet and Bai's probiotic BS139

[0051] In order to test the difference between the oral probiotic products of brand A and the probiotics BS139, in Example 1, a well-known brand A in the country was selected for comparison with the oral health food powder containing probiotics BS139, and the following experimental procedures were followed. Perform probiotic counts.

[0052] 1. Weigh the oral tablets of brand A and the oral health food containing BS139, respectively, and dissolve the tablets and powder with sterile water at a ratio of 20× (20 times).

[0053] 2. Dilute the dissolved oral lozenges of brand A and the probiotics containing BS139 by 10 times (10×) serially, and smear the diluted bacteria solution until cultured in MRS and LB petri dishes, among which the MRS petri dish Treat in an anaerobic environment and incubate at 37°C for 24 hours.

[0054] 3. Calculate the number of bacteria respe...

Embodiment 2

[0058] Example 2: The influence of the probiotics contained in the oral lozenges of brand A and the probiotics BS139 on the pH value of pathogenic bacteria and the adsorption status of the glass tube surface

[0059] In order to test the difference between the probiotics contained in the oral lozenges of brand A and the probiotics BS139, Example 2 is to compare the oral health food powder of the oral tablets of brand A with the oral health food powder containing the probiotics BS139, as follows Experimental procedure The co-cultivation experiments of A brand of Kouhan Tablets and Bordet's probiotic BS139 and S.mutans pathogenic bacteria were carried out respectively, in order to explore the pH value effect of A brand of Kou Han Tablets and Bo's probiotic BS139 on pathogenic bacteria and the adsorption status of the glass tube surface.

[0060] 1. Prepare a 1% sucrose and 1% peptone culture solution, adjust the pH to 7.0, and sterilize it for later use.

[0061] 2. Weigh the or...

Embodiment 3

[0073] Example 3: Antagonistic experiment between the Burger's probiotic BS139 and the S.mutans pathogenic bacteria

[0074] Example 3 Antagonism experiments were used to investigate whether the probiotic bacterium BS139 has the secretion of antibiotic substances, and whether the probiotic bacterium BS139 can absorb sucrose and other nutrient sources through its superior growth rate, thereby reducing the pathogenic bacteria of S.mutans, according to the following experimental steps The antagonism experiment between BS139 and S.mutans pathogenic bacteria was carried out.

[0075] 1. Take the activated S.mutans pathogenic bacteria liquid and apply it to a TSA petri dish, cover it with 0.75% agaric gum after drying, and set it aside after solidification.

[0076] 2. Place a paper disc on the petri dish, and add 30 μl of different concentrations of BS139 bacterial solution of Burger's probiotics dropwise.

[0077] 3. Place it at 37°C for 24 hours and then observe.

[0078] refer...

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Abstract

The present invention discloses a composition for restraining growth of oral pathogenic bacteria. The composition contains an effective dose of bacillus subtilis (berlese probiotics BS 139), a diluents, an excipient or a carrier. The growth of the oral pathogenic bacteria is restrained through antibiotics in short-chain polypeptides secreted by the bacillus subtilis. The oral pathogenic bacteria are streptococcus mutans, and the bacillus subtilis are bacterial strains which are preserved in the NRRL of Agricultural Research Service and whose preservation No. is NRRL No. B-50347. Additionally, the bacillus subtilis can also be used as a method for restraining the growth of the oral pathogenic bacteria, where the abovementioned composition is applied to individuals.

Description

technical field [0001] The invention relates to an application of probiotics for oral health care, in particular to a composition and method for inhibiting the growth of oral pathogenic bacteria. Background technique [0002] High-sugar and high-fat refined diet is a common dietary pattern nowadays. In addition to increasing the increase in related chronic diseases such as gastrointestinal and cardiovascular diseases, eating high-sugar foods for a long time puts the oral cavity in an environment with increased risk factors middle. The application of probiotics in gastrointestinal health has been practiced for many years, and more new functions applied to human health are constantly being discovered, among which the research on oral health is also one of the projects. Therefore, it has become a new trend to combine probiotics with oral health and stomach health. [0003] (1) Microorganisms and oral cavity [0004] Streptoccocus mutans is a resident bacterium in the oral ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/64A23L1/29A61Q11/00A61P1/02A23L33/00
Inventor 林秀霞
Owner 林秀霞
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