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Serratia marcescens and application thereof

A technology of Serratia marcescens and physiological race, applied in the field of microorganisms, can solve the problems of reduced inhibitory activity of rubber powdery mildew and banana wilt, lack of field test results, poor genetic stability, etc. Effects of polluting the environment and reducing the disease index

Active Publication Date: 2013-10-02
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are reports on biological control of banana fusarium wilt at home and abroad, but the selected bio-control bacteria are all fungi such as Bacillus monospora and Trichoderma, and only indoor pot plant control tests are carried out, and there is still no field test results, so it is limited as a bio-control agent. Preparations are used in production
In addition, with the increase of the number of passages, the inhibitory activity of the currently screened microorganisms against rubber powdery mildew and banana wilt gradually decreases, and their genetic stability is poor. biological strain

Method used

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  • Serratia marcescens and application thereof
  • Serratia marcescens and application thereof
  • Serratia marcescens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the isolation method of Serratia marcescens of the present invention

[0024] Use alcohol-sterilized scissors to cut the stems of healthy rubber buds, and immediately dry the latex flowing out of the cuts of the stems with fire to prevent the latex from continuing to flow out. Take the rubber stems back to the laboratory, rinse them with distilled water, and cut them into long pieces. About 4cm section, soak in 75% alcohol for 1min, soak in 0.2% mercuric chloride for 10min, and finally rinse with sterilized water for 5 times. After absorbing the excess water with sterile filter paper, use a sterilized scalpel to cut the rubber stem into two sections, drop 1 drop of latex onto the LB plate and smear it, take the last washing solution as the control, and incubate at 26°C , observed day by day. The above operation steps were all performed under sterile conditions. After 8 days of culture, there was no colony in the control, but a red bacterial strain grew o...

Embodiment 2

[0025] Embodiment 2: Serratia marcescens physiology and biochemistry of the present invention and the determination of 16S DNA sequence

[0026] 1. Physiological and biochemical detection

[0027] Detect the growth of ITBB B5-1 on LB plates, colony shape and color, Gram staining, spore staining and flagella staining, etc., contact enzymes, V. P. Reaction, starch hydrolysis, nitrate reduction reaction, gelatin liquefaction reaction, oxidase, phenylalanine deaminase, citrate utilization, urease test, propionate utilization and other tests, the method refers to " The method on pages 353-390 of the Handbook of Identification of Common Bacteria Systems.

[0028] The test results are as follows:

[0029] It grows well on LB medium, and the colony is bright red, slightly raised, round, and the surface is wet and smooth, sticky. The bacterium is spherical to short rod in shape, 0.5-1um in diameter, Gram-negative, with flagella all over the body, able to move, without capsule, and ...

Embodiment 3

[0034] Embodiment 3: Serratia marcescens of the present invention is to the inhibition test of rubber powdery mildew spore germination

[0035] Experimental design: the Serratia marcescens of the present invention was inoculated into LB culture dishes for activation, and the dishes were transferred once every 24 hours, each time as one generation of culture, and the dishes were transferred 40 times in total. The inhibitory effect of endophytic Serratia marcescens on the germination of rubber powdery mildew spores of the 1st, 10th, 20th, 30th and 40th generations was determined by the hanging drop method. The results are shown in Table 1.

[0036] Table 1 Serratia marcescens of the present invention inhibits the germination of rubber powdery mildew spores

[0037]

[0038] As can be seen from Table 1, Serratia marcescens described in the present invention inhibits the EC of rubber powdery mildew spore germination from the 1st generation to the 40th generation. 50 The range ...

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Abstract

The invention relates to the field of microorganism, and in particular discloses a serratia marcescens and an application thereof. The serratia marcescens is preserved in the China General Microbiological Culture Collection Center and has the preservation number of CGMCC No.7416. The serratia marcescens is transferred to the 40th generation and still has higher activity in inhibiting rubber powdery mildew and banana vascular wilt bacterial strain spores; the serratia marcescens has higher genetic stability, the rubber powdery mildew prevention effect of the serratia marcescens reaches a chemical prevention effect, and the serratia marcescens has an obvious prevention effect on banana vascular wilt; and moreover, the serratia marcescens cannot pollute the environment, is ecological and safe, and can be widely applied to prevent the rubber powdery mildew and the banana vascular wilt and prepare rubber powdery mildew and banana vascular wilt prevention preparations.

Description

technical field [0001] The invention relates to the field of microbes, more specifically to a Serratia marcescens and its application. Background technique [0002] Hevea brasiliensis Muell-Arg, also known as Hevea brasiliensis Muell-Arg, is a perennial cross-pollinated tree of Euphorbiaceae and is an economic crop for the production of natural rubber. Banana is one of the four famous fruits in the world and the most important tropical fruit with high medicinal value. Rubber trees and bananas are both characteristic economic crops in Hainan, my country. They have been planted on a large scale and are one of the important pillar industries in my country's hot areas. [0003] With the large-scale planting of rubber trees and bananas, the invasion of plant diseases followed. Among them, rubber powdery mildew is one of the main diseases of rubber trees. It is widely prevalent in various rubber planting areas. It mainly harms the young leaves, buds and inflorescences of rubber ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/00A01P3/00A01P1/00C12R1/43
Inventor 张家明谭德冠孙雪飘郑鹏付莉莉韩冰莹
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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