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Collagen hydrogel for injection and preparation method thereof

A collagen and collagen polypeptide technology, which is applied in the field of collagen hydrogel for injection and its preparation, can solve the problems of strong biological toxicity, time-consuming, and difficulty in dissolving, so as to reduce the damage to the injection site and the human body, and reduce the degradation. speed, and the effect of improving retention time

Inactive Publication Date: 2013-10-02
SHAANXI GIANT BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN101005865A discloses a preparation method of collagen sponge, crosslinking with glutaraldehyde, but there will be high concentration of glutaraldehyde residue in the product, which will cause strong biological toxicity and endanger human health
However, the hydroformylated polysaccharides such as dialdehyde starch and dialdehyde chondroitin sulfate are not easy to dissolve at room temperature, and have certain cytotoxicity, and the reaction time of this method is 1-7 days at low temperature, and it is time-consuming to thaw the product afterwards. , the production efficiency is relatively low

Method used

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  • Collagen hydrogel for injection and preparation method thereof
  • Collagen hydrogel for injection and preparation method thereof
  • Collagen hydrogel for injection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Step 1: dissolving collagen in an alkaline solution to prepare a solution with a collagen concentration of 5%.

[0062] Collagen is selected from full-length collagen extracted from animal tissues.

[0063] The alkaline solution is sodium hydroxide solution with a molar volume concentration of 0.05 mol / L.

[0064] Step 2: Add epoxy compound as a crosslinking agent, the amount added is 0.5% of the solution mass, the reaction temperature is 35°C, the reaction time is 10 hours, and left to cool.

[0065] The epoxy compound is selected from 1,4-butanediol diglycidyl ether.

[0066] Step 3: Pulverizing the obtained product.

[0067] Select extrusion as the crushing method.

[0068] Step 4: Wash the obtained granular product in a buffer solution 10 times its volume for 1 day.

[0069] The buffer is a phosphate buffer with a molar volume concentration of 0.01 mol / L and a pH of 6.

[0070] Step 5: Remove the buffer by centrifugation, and collect the precipitate to obtain a...

Embodiment 2

[0072] Step 1: dissolving collagen in an alkaline solution to prepare a solution with a collagen concentration of 5%.

[0073] Collagen is selected from collagen peptides extracted from animal tissues.

[0074] The alkaline solution is sodium hydroxide solution with a molar volume concentration of 0.1 mol / L.

[0075] Step 2: Add epoxy compound as a crosslinking agent, the amount added is 2% of the solution mass, the reaction temperature is 40°C, the reaction time is 10 hours, and left to cool.

[0076] Epoxy compounds are selected from ethylene glycol diglycidyl ether.

[0077] Step 3: Pulverizing the obtained product.

[0078] Select extrusion as the crushing method.

[0079] Step 4: Wash the obtained particle product in a buffer solution 20 times its volume for 1 day.

[0080] The buffer is a phosphate buffer with a molar volume concentration of 0.02 mol / L and a pH of 6.

[0081] Step 5: Remove the buffer by centrifugation, and collect the precipitate to obtain a granul...

Embodiment 3

[0083] Step 1: dissolving collagen in alkaline solution to prepare a solution with a collagen mass concentration of 10%.

[0084] Collagen is selected from gelatin extracted from animal tissue.

[0085] The alkaline solution is potassium hydroxide solution with a molar volume concentration of 0.2 mol / L.

[0086] Step 2: Add epoxy compound as cross-linking agent, the addition amount is 4% of the solution mass, the reaction temperature is 45°C, the reaction time is 20 hours, and left to cool.

[0087] The epoxy compound is selected from 1,6-hexanediol diglycidyl ether.

[0088] Step 3: Pulverizing the obtained product.

[0089] The grinding method is selected as grinding.

[0090] Step 4: Wash the obtained particle product in a buffer solution 30 times its volume for 1 day.

[0091] The buffer is a phosphate buffer with a molar volume concentration of 0.03 mol / L and a pH of 6.

[0092] Step 5: Remove the buffer by centrifugation, and collect the precipitate to obtain a gran...

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Abstract

The invention relates to collagen hydrogel for injection and a preparation method thereof. Organic solvents, organic sterilizing agents and the like are frequently introduced or remained in the existing collagen cross-linking technology; the product safety and the biocompatibility are poor. According to the invention, collagen is used as the raw material; hydrogel is formed by carrying out intramolecular or intermolecular cross-linking reaction of the raw material by using a cross-linking agent; enzyme or an epoxy compound is selected as the cross-linking agent; influence of the cross-linking agent remained in a product on performances of the product is lightened or eliminated in a cleaning or inactivating manner. The product disclosed by the invention is free from biological toxicity; damage of the product on human body is reduced; simultaneously, because of intramolecular or intermolecular cross-linking, the degradation rate of collagen is reduced; the in-vivo retention time of collagen is increased; furthermore, good performances for promoting new cells to form and grow in collagen in hydrogel are not influenced.

Description

technical field [0001] The invention relates to a hydrogel, in particular to a collagen hydrogel for injection and a preparation method thereof. Background technique [0002] Collagen is a kind of biological polymer substance, which widely exists in mammals. It is a very important structural protein in connective tissue and the main component of extracellular matrix. Collagen has the chemical properties of general proteins, and can undergo amino reaction, carboxyl reaction, hydroxyl reaction, color reaction, cross-linking reaction, etc., and has a specific isoelectric point. And because there are many polar side groups on the surface of collagen molecules, its water absorption performance is very good, and it can bind water more than 10 times its own mass, but itself is insoluble in water. [0003] Human-like collagen (Human-like Collagen) is the reverse transcription of human collagen mRNA into cDNA, and a section of the gene after digestion is recombined in E.coli (Esche...

Claims

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Application Information

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IPC IPC(8): C08L89/00C08J3/075C08J3/24A61L27/24A61L27/52
Inventor 范代娣马晓轩张婧婧
Owner SHAANXI GIANT BIOTECHNOLOGY CO LTD
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