Determination method of potency of traditional Chinese medicine zedoary turmeric oil by using metabolomics

A technology of metabolomics and determination method, which is applied in the field of determination of the efficacy of traditional Chinese medicine Curcuma curcuma oil by metabolomics, can solve the problems of clear understanding and influence of the drug effects of Wen Curcuma radix, and achieve the effects of improving metabolic changes and convenient operation.

Inactive Publication Date: 2013-09-25
WENZHOU MEDICAL UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to provide a method for measuring the efficacy of traditional Chinese medicine zedoary oil by applying metabolomics, aiming to solve the problem that there are many active ingredients in warming zedoary oil and having multiple drug effects ;Each active compound component may have multiple targets and directly affect multiple biological processes; there is synergy and mutual influence between components, and the ideological basis of traditional Chinese medicine compatibility is synergy. A single chemical component and pharmacological index The problem of not being able to clearly understand the drug effect of warm curcuma

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Determination method of potency of traditional Chinese medicine zedoary turmeric oil by using metabolomics
  • Determination method of potency of traditional Chinese medicine zedoary turmeric oil by using metabolomics
  • Determination method of potency of traditional Chinese medicine zedoary turmeric oil by using metabolomics

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0042] Serum sample preparation and acquisition 1 H NMR spectral data;

[0043] NMR data processing and pattern recognition analysis;

[0044] Application of SPSS13.0 software for statistical analysis.

[0045] As an optimization scheme of the embodiment of the present invention, animal modeling: after adaptive feeding of rats for 1 week, they were randomly divided into: sham operation group, model group and drug administration group;

[0046] The renal interstitial fibrosis model caused by unilateral ureteral obstruction was adopted, and the specific process was as follows: After the rat was anesthetized by intraperitoneal injection of 10% chloral hydrate 3mL / Kg, the hair on the lower part of the left rib of the back of the rat was removed, and the left back was selected. An incision was made at 0.5 cm below the ribs, and the skin was incised to the abdominal cavity in turn, and the kidney and ureter were freed. The renal pelvis was ligated twice with 4-0 silk suture, and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a determination method of the potency of a traditional Chinese medicine zedoary turmeric oil by using metabolomics. The method comprises the steps that: an unilateral ureteral obstruction induced renal interstitial fibrosis model is adopted, an animal modeling is carried out; processing and sampling are carried out after animal modeling is carried out for 3 days, 7 days, 14 days, 21 days, and 28 days; animal kidney tissues are fetched; kidney tissue pathological changes are observed by using an optical microscope; serum samples are prepared, and 1H NMR spectral data is obtained; NMR data processing and pattern recognition analysis are carried out; and analysis is carried out by using SPSS13.0 software. According to the invention, information of large amount of endogenous metabolites in bodies can be obtained with the metabolomic technology, and metabolite changes of potencies of various effective components in zedoary turmeric oil upon various targets can be screened. Therefore, the potency of zedoary turmeric oil can be comprehensively evaluated. The invention provides an excellent method for evaluating zedoary turmeric oil potency.

Description

technical field [0001] The invention belongs to the technical field of research on curcuma oil, a traditional Chinese medicine, and in particular relates to a method for measuring the efficacy of curcuma oil, a traditional Chinese medicine, by applying metabolomics. Background technique [0002] Zedoary oil has a wide range of pharmacological activities and can be used in the fields of anti-tumor, anti-virus, anti-bacteria and anti-inflammation. However, due to historical limitations, the therapeutic effect of traditional Chinese medicine on the warming of Zezhu often stays in the summary of experience and macroscopic description, and lacks the basis of scientific experiments. To explain and verify, the mechanism of action of zedoary oil should be "multi-component, multi-target" in theory, but it often lacks effective research methods and technical means to prove it. The characteristics of the function of the body bring great difficulties to the study of the exact molecular ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N24/08
Inventor 高红昌赵良才张海燕贾建敏管咪咪朱环郑涌泉王娜魏婷婷
Owner WENZHOU MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap