LED (light emitted diode) line scanning optical system applied to confocal microscopy

Abstract

The invention discloses an LED (light emitted diode) line scanning optical system applied to a confocal microscopy. The LED line scanning optical system comprises a high-luminance LED light source module which is provided with an LED luminous chip, wherein a dodging coupling module is arranged right ahead of the LED luminous chip, a focusing lens is arranged in front of the dodging coupling module, a first slit is formed in front of the focusing lens, and a dichroscope, an imaging system, a second slit and an object plane are successively arranged behind the first slit. By adopting the technical scheme disclosed by the invention, the ideal line light source excitation is realized, the LED line scanning optical system is completely equal to a plurality of laser light sources large in size and high in price in the function aspect, and more importantly, the line light source excitation which can not be realized by the laser light sources is obtained; the complexity and the debugging difficulty of the system are greatly reduced.

Description

technical field [0001] The invention belongs to the field of optical imaging, in particular to an LED line scanning optical system applied in confocal microscopy. Background technique [0002] Since its invention, confocal microscopy has been deeply researched and widely used, especially after it has been proved by experiments that it has unique depth resolution and can realize optical tomography for biological samples. The optical tomography property of confocal microscopy allows people to cut thick samples into thin slices in a non-destructive way, and then use a computer to synthesize the tomographic images into a three-dimensional image. [0003] At present, confocal microscopy mainly adopts laser confocal point scanning method, adopts laser light source as excitation light source, removes interfering light through confocal optical path, and converges laser light into points to excite fluorescence, which has high detection sensitivity, but imaging requires two dimension...

AI Technical Summary

Problems solved by technology

[0006] The traditional confocal point scanning imaging mode using a laser light source has the following disadvantages: (1) The system is composed of large-volume optical components (such as multiple sets of lasers), which makes the system configuration very complicated, bulky and expensive; (2) If the laser beam There is a deviation between the convergence point and the pinhole alignment, which will cause distortion and aberration, and the submicron movement will cause aberration and resolution degradation; (3) The measurement time is long, and each surface of the object to be measured needs to be measured Point-by-point two-dimensional scanning and synthesizing three-dimensional images through axial scanning

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