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A kind of comprehensive utilization method of camellia oleifera shell

A technology of camellia oleifera shell and solid-liquid separation, applied in the direction of microorganism-based methods, biochemical equipment and methods, separation/purification of carbonyl compounds, etc., to achieve the effect of reducing environmental pressure

Active Publication Date: 2016-03-09
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, how to use all three components in an integrated process to produce high value-added products is still a problem

Method used

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  • A kind of comprehensive utilization method of camellia oleifera shell
  • A kind of comprehensive utilization method of camellia oleifera shell
  • A kind of comprehensive utilization method of camellia oleifera shell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0040] Example 2: Enzymatic hydrolyzate fermentation to prepare fuel ethanol

[0041]The solid residue after the Camellia oleifera shell alkali pretreatment is enzymatically hydrolyzed with cellulase, and the obtained monosaccharide is used for ethanol fermentation. Glucose and xylose were contained in the enzymatic hydrolyzate, and ethanol fermentation was carried out by using a strain of Pichia stipitis CBS5776 capable of utilizing glucose and xylose. The enzymatic hydrolyzate fermentation includes direct ethanol fermentation of the enzymatic hydrolyzate and ethanol fermentation after the enzymatic hydrolyzate is concentrated by 1 times. Fermentation medium composition: 5g (NH 4 ) 2 SO 4 , 3gKH 2 PO 4 , 0.5gMgSO 4 ·7H 2 O, 30 mg EDTA, 9 mg ZnSO 4 ·7H 2 O, 2mgMnCl 2 ·2H 2 O, 0.6 mg CoCl 2 ·6H 2 O, 0.6 mg CuSO 4 ·5H 2 O, 0.8mgNa 2 MoO 4 ·H 2 O, 9 mg CaCl 2 ·2H 2 O, 6 mg FeSO 4 ·7H 2 O, 2mgH 3 BO 3 , 0.2mgKI. Adjust pH to 6.0 with sodium hydroxide. The...

Embodiment 3

[0050] Example 3: Separation of xylan and lignin by adjusting pH

[0051] Take 30mL of the black liquor after the best pretreatment (Example 1) in a 50mL centrifuge tube, add 72% sulfuric acid to adjust the pH value of the black liquor to about 1, 2, 3, 4, 5, 6, and 7 respectively. . After equilibration, it was centrifuged at 10000 r / min for 5 min to carry out solid-liquid separation, and the solid precipitate and supernatant were collected respectively, and the volume of the supernatant was measured for use.

[0052] Take 10 mL of the supernatant into a 50 mL conical flask, add 10 mL of 8% sulfuric acid, seal it with parafilm, react at 121 °C for 60 min, take it out after cooling, use G 3 The filtrate was collected and the volume was recorded. Liquid part: mainly analyze the content of acid-soluble / acid-insoluble lignin and xylan content.

[0053] The obtained solid precipitate was washed with distilled water and the volume was adjusted to 25 mL. Take 10 mL of the well-mi...

Embodiment 4

[0055] Example 4: Preparation of the chemical vanillin

[0056] The chemical vanillin is prepared by the method of pretreating lignin in black liquor by alkaline oxidation of Camellia oleifera. In the process proposed by the present invention, xylan and lignin should be used as a whole, but it is unknown whether to first oxidatively degrade lignin to prepare vanillin, then enzymatically hydrolyze xylan to prepare xylo-oligosaccharide, or firstly enzymatically hydrolyze xylan to prepare Xylo-oligosaccharide and lignin oxidation to produce vanillin? Therefore, the results of the preparation of vanillin by oxidation of different samples under alkaline conditions (sodium hydroxide concentration of 2 mol / L, 170 °C, 2 h) are shown in Table 4.

[0057] Table 4 Compositional analysis of different sample preparation chemicals

[0058]

[0059] Note: lignin oxidation conditions: 30mL black liquor, 2.0mol / LNaOH, 0.98gCuO, 170℃, 2h. Xylanase hydrolysis conditions: xylanase dosage 10...

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Abstract

The invention discloses a comprehensive utilization method of oil-tea camellia shells. The method comprises the following steps: crushing oil-tea camellia shells, performing alkaline pretreatment, and performing solid-liquid separation to obtain black liquor and solid residues; performing enzymolysis on the solid residues through cellulase to prepare monosaccharide and performing alcohol fermentation, and preparing ethanol; regulating pH of the black liquor, and performing solid-liquid separation to obtain residues and a vanillic aldehyde solution; and performing enzymolysis on the residues through xylanase to prepare an xylooligosaccharide solution and residues, and oxidizing the residues to prepare the vanillic aldehyde. According to the comprehensive utilization method, the solid residues obtained by solid-liquid separation are hydrolyzed by cellulase and further fermented to prepare fuel ethanol by using the condition that the oil-tea camellia shells are pretreated by sodium hydroxide; the black liquor obtained by solid-liquid separation is neutralized to the pH of 6, wherein the supernatant obtained by solid-liquid separation contains high-concentration vanillic aldehyde (lignin oxidization is not required), enzyme hydrolysis is carried out on the precipitation to prepare xylooligosaccharide by employing xylanase containing a small amount of beta-xylosidase, and further the residues obtained by enzymolysis are further oxidized to prepare the vanillic aldehyde. Therefore, according to research of the process, the biomass can be totally utilized for preparing the fuel ethanol, xylooligosaccharide and vanillic aldehyde finally.

Description

technical field [0001] The invention relates to the technical field of agricultural waste treatment, in particular to a comprehensive utilization method of Camellia oleifera shells. Background technique [0002] my country is a big agricultural country and a country with a shortage of oil resources. The high-value utilization of my country's abundant biomass resources is an important guarantee for our country to implement sustainable development strategies. It is of great significance and can establish a new industry in my country, adjust the industrial structure, develop agriculture and increase farmers' income, and provide a new way to solve the "three rural" problems that the central government attaches great importance to. [0003] Camellia oleifera is a unique woody oil crop in my country, mainly distributed in the southern provinces of China. At present, the planted area of ​​camellia oleifera in China is about 4 million hectares. With the adjustment of my country's a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12P19/14C12P19/02C12P19/12C12P19/00C12P19/04C07C47/58C07C45/78C12R1/84
CPCY02E50/10
Inventor 朱均均朱圆圆蒋发现勇强徐勇李鑫余世袁
Owner NANJING FORESTRY UNIV
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