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Comprehensive utilization method of oil-tea camellia shells

A technology of camellia oleifera shell and solid-liquid separation, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, separation/purification of carbonyl compounds, etc., to achieve high-value utilization and reliability, and to reduce environmental pressure.

Active Publication Date: 2013-09-25
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, how to use all three components in an integrated process to produce high value-added products is still a problem

Method used

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  • Comprehensive utilization method of oil-tea camellia shells
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  • Comprehensive utilization method of oil-tea camellia shells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Alkaline pretreatment of camellia oleifera shell

[0027] (1) Camellia oleifera husks (the contents of cellulose, xylan and lignin are 14.02%, 28.61% and 44.35% respectively) are crushed to 0.2-0.4mm and set aside.

[0028] (2) Sodium hydroxide pretreatment: The pulverized camellia oleifera shells were pretreated with sodium hydroxide, and orthogonal experiments were used to optimize the pretreatment conditions. The main variables and conditions were as follows: NaOH concentration (10%, 12%, 14 %), temperature (110, 115, 120°C), time (90, 120, 150min), liquid-solid ratio (10.0, 12.5, 15.0). The details are shown in Table 1.

[0029] Table 1 Orthogonal experiment L 9 (3 4 ) factor level table

[0030]

[0031] (3) Solid-liquid separation: After pretreatment, use G 1 Suction filtration through the filter for solid-liquid separation, and 300 mL of distilled water was added to wash the solid residue three times, and the filtrate was collected for later use...

Embodiment 2

[0040] Example 2: Preparation of fuel ethanol by fermentation of enzymatic hydrolyzate

[0041]The solid slag pretreated by the camellia husk alkali method is hydrolyzed by cellulase, and the obtained monosaccharide is used for ethanol fermentation. Glucose and xylose are contained in the enzymatic hydrolyzate, and Pichia stipitis, a strain capable of utilizing glucose and xylose ( Pichia stipitis CBS 5776) for ethanol fermentation. The enzyme hydrolyzate fermentation includes the direct ethanol fermentation of the enzyme hydrolyzate and the ethanol fermentation after the enzyme hydrolyzate is concentrated by one time. Fermentation medium composition: 5g (NH 4 ) 2 SO 4 , 3g KH 2 PO 4 , 0.5g MgSO 4 ·7H 2 O, 30mg EDTA, 9mg ZnSO 4 ·7H 2 O, 2 mg MnCl 2 2H 2 O, 0.6 mg CoCl 2 ·6H 2 O, 0.6 mg CuSO 4 ·5H 2 O, 0.8 mg Na 2 MoO 4 ·H 2 O, 9 mg CaCl 2 2H 2 O, 6mg FeSO 4 ·7H 2 O, 2 mg H 3 BO 3 , 0.2 mg KI. Adjust the pH to 6.0 with sodium hydroxide. The initial ...

Embodiment 3

[0050] Embodiment 3: adjust pH value to separate xylan and lignin

[0051] Take 30mL of black liquor after optimal pretreatment (Example 1) in a 50mL centrifuge tube, add 72% sulfuric acid to adjust the pH value of the black liquor to about 1, 2, 3, 4, 5, 6, 7 respectively . After equilibrium, centrifuge at 10,000 r / min for 5 minutes, then separate the solid from the liquid, collect the solid precipitate and the supernatant, and measure the volume of the supernatant for later use.

[0052] Take 10mL of supernatant in a 50mL Erlenmeyer flask, add 10mL of 8% sulfuric acid, seal it with a parafilm, react at 121°C for 60min, take it out after cooling, and use G 3 Filter through a glass sand funnel, collect the filtrate and record the volume. Liquid part: mainly analyze the content of acid-soluble / acid-insoluble lignin and xylan.

[0053] The obtained solid precipitate was washed with distilled water and the volume was adjusted to 25 mL. Take 10mL of uniformly mixed suspension ...

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Abstract

The invention discloses a comprehensive utilization method of oil-tea camellia shells. The method comprises the following steps: crushing oil-tea camellia shells, performing alkaline pretreatment, and performing solid-liquid separation to obtain black liquor and solid residues; performing enzymolysis on the solid residues through cellulase to prepare monosaccharide and performing alcohol fermentation, and preparing ethanol; regulating pH of the black liquor, and performing solid-liquid separation to obtain residues and a vanillic aldehyde solution; and performing enzymolysis on the residues through xylanase to prepare an xylooligosaccharide solution and residues, and oxidizing the residues to prepare the vanillic aldehyde. According to the comprehensive utilization method, the solid residues obtained by solid-liquid separation are hydrolyzed by cellulase and further fermented to prepare fuel ethanol by using the condition that the oil-tea camellia shells are pretreated by sodium hydroxide; the black liquor obtained by solid-liquid separation is neutralized to the pH of 6, wherein the supernatant obtained by solid-liquid separation contains high-concentration vanillic aldehyde (lignin oxidization is not required), enzyme hydrolysis is carried out on the precipitation to prepare xylooligosaccharide by employing xylanase containing a small amount of beta-xylosidase, and further the residues obtained by enzymolysis are further oxidized to prepare the vanillic aldehyde. Therefore, according to research of the process, the biomass can be totally utilized for preparing the fuel ethanol, xylooligosaccharide and vanillic aldehyde finally.

Description

technical field [0001] The invention relates to the technical field of agricultural waste treatment, in particular to a method for comprehensive utilization of camellia oleifera shells. Background technique [0002] my country is a large agricultural country and a country short of oil resources. The high-value utilization of my country's rich biomass resources is an important guarantee for our country to implement sustainable development strategies. It is not only very important for ensuring my country's energy, national security, and environmental protection. It is of great significance, and can establish a new industry in our country, adjust the industrial structure, develop agriculture and increase farmers' income, and provide a new way to solve the "three rural" issues that the central government attaches great importance to. [0003] Camellia oleifera is a unique woody oil crop in my country, mainly distributed in the southern provinces of China. At present, the nationa...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12P19/14C12P19/02C12P19/12C12P19/00C12P19/04C07C47/58C07C45/78C12R1/84
CPCY02E50/10
Inventor 朱均均朱圆圆蒋发现勇强徐勇李鑫余世袁
Owner NANJING FORESTRY UNIV
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