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General flavone extract of seeds of nigella damascena l., nigella sativa l. or nigella glandulifera freyn et sint., and preparation method and use thereof

A technology of Nigella sativa seed and total flavonoids is applied in the field of Nigella sativa seed total flavonoid extract and preparation thereof, and achieves the effects of strong operability, significant medicinal effect and simple preparation method

Inactive Publication Date: 2013-09-25
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no literature reports on the preparation technology and anti-cancer, anti-platelet aggregation and anti-inflammatory effects of its total flavonoids

Method used

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  • General flavone extract of seeds of nigella damascena l., nigella sativa l. or nigella glandulifera freyn et sint., and preparation method and use thereof
  • General flavone extract of seeds of nigella damascena l., nigella sativa l. or nigella glandulifera freyn et sint., and preparation method and use thereof
  • General flavone extract of seeds of nigella damascena l., nigella sativa l. or nigella glandulifera freyn et sint., and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Weigh 5 kg of nigrum nigella seeds medicinal materials, crush them, put them in an extraction tank, add 40Kg of 70% ethanol-water solution, and extract 3 times at 80°C for 2 hours each time; combine the extracts and concentrate under reduced pressure until soaking Paste; reflux degreasing with petroleum ether for 2 hours each time, repeat 3 times; evaporate petroleum ether, and extract with ethyl acetate until the extract layer is colorless; the collected ethyl acetate is concentrated under reduced pressure; the concentrated solution is D101 type After adsorption on the porous resin column, it is eluted with 10 times the resin volume of deionized water, and then with 6 times the resin volume of 10%, 30%, 50%, 70% ethanol-water solution in sequence; Collect the ethanol-water eluate with a volume percentage of 70%, concentrate under reduced pressure, and freeze-dry, and the brown-yellow powder obtained is the total flavonoid extract of Nigella sativa; after testing, the mas...

Embodiment 2

[0041] Weigh 5kg of nigrum nigella seed medicinal material, crush it, place it in an extraction tank, add 50Kg of 70% ethanol-water solution, extract 3 times at 80°C, 2 hours each time; combine the extracts, concentrate under reduced pressure until soaking Paste; reflux degreasing with petroleum ether for 2 hours each time, repeat 3 times; evaporate petroleum ether, and extract with ethyl acetate until the extract layer is colorless; concentrate the collected ethyl acetate under reduced pressure to the shape of extract; take appropriate amount of pure Dissolve in water ultrasonically; after adsorbing with AB-8 type macroporous resin column, first elution with 10 times the volume of the resin, and then use 6 times the volume of the resin. The volume percentage is 10%, 30%, 50%, 70% ethanol-water solution is eluted in sequence; 70% ethanol-water eluate is collected, concentrated under reduced pressure, and freeze-dried. The brown powder obtained is the total flavonoid extract of N...

Embodiment 3

[0043] Weigh 1kg of nigrum nigella seeds medicinal materials, pulverize, place in an extraction tank, add 10Kg of 95% ethanol-water solution, and extract 3 times at 80°C for 2 hours each time; combine the extracts and concentrate under reduced pressure until soaking Paste; reflux degreasing with ether for 2 hours each time, repeat 3 times; evaporate the ether and extract with ethyl acetate until the extract layer is colorless; concentrate the collected ethyl acetate under reduced pressure to the shape of extract; take an appropriate amount of pure water and ultrasound Dissolve; after adsorbing with HPD-600 type macroporous resin column, first elution with 10 times the volume of the resin, and then use 6 times the volume of the resin. The volume percentages are 20%, 40%, 60%, 95% The ethanol-water solution was eluted in sequence; the 95% ethanol-water eluate was collected, concentrated under reduced pressure, and freeze-dried. The resulting brown powder was the total flavonoid ex...

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Abstract

The invention discloses a general flavone extract of seeds of nigella damascena l., nigella sativa l. or nigella glandulifera freyn et sint., and a preparation method and a use thereof. The extract is extracted from the seeds of nigella damascena l., nigella sativa l. or nigella glandulifera freyn et sint., and the mass percentage of general flavones is 50%-90% of that of the extract. The preparation of the extract comprises: extracting the seeds by water, alcohol or alcohol-water solution, then degreasing by an ether solvent, leaching by an organic solvent, carrying out adsorption and desorption by a macroporous resin column, concentrating, and drying to obtain the extract. The extract provided by the invention has anti-malignant tumor, anti-platelet aggregation and anti-inflammatory activities, is expected to develop into an anti-malignant tumor drug or an anti-platelet aggregation drug or an anti-inflammatory drug, and has significantly medicinal effect; in addition, the preparation method of the general flavone extract of the seeds of nigella damascena l., nigella sativa l. or nigella glandulifera freyn et sint. is simple, has high practicality, and is suitable for industrialized production requirements.

Description

Technical field [0001] The invention relates to a Nigella sativa total flavonoid extract, a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine. Background technique [0002] Nigella is an annual herb of the genus Nigella in the Ranunculaceae family Nigella damascena L. or Nigella sativa L. or Nigella sativa L. or Nigella glandulifera Freyn et Sint .Seed. Plants are harvested when the fruits are mature in summer and autumn, dried in the sun, lay down the seeds, removed impurities, and dried in the sun. Nigella Seed has the functions of diuresis, invigorating the kidney and brain, promoting blood circulation, detoxification, lowering the breast, relieving cough, and anti-aging. Its nature is sweet, pungent, and warm. It is included in various editions of the Chinese Pharmacopoeia as a traditional Uyghur medicine. It is used for the treatment of chest tightness, shortness of breath, breast swelling, tinnitus and reduced m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71A61P7/02A61P29/00A61P35/00
Inventor 章丹丹卞卡
Owner SHANGHAI UNIV OF T C M
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