Method for detecting percutaneous absorption efficiency of active component of cosmetic
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A technology of active ingredients and detection methods, applied in the field of biomedicine, can solve problems such as time-consuming, cumbersome operations, and heavy workload, and achieve the effects of improving safety and operability, improving detection efficiency, and shortening detection time
Inactive Publication Date: 2013-09-18
LAFANG CHINA CO LTD
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Because the Franz diffusion cell method requires a large area of skin (diameter 16mm), it is necessary to collect and process samples separately, which is cumbersome and heavy workload; moreover, it takes a long time to reach the stable state of transmission, and the entire detection cycle takes 24 hours The above has seriously affected the development efficiency of the penetration-promoting formula
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Embodiment 1
[0023] Example 1 Correlation Analysis of Conductivity and Permeability of Inulin Percutaneous Absorption
[0024] 1.1 Experimental materials and reagents
[0025] Reagent: inulin (CAS registration number: 9005-80-5), final concentration 100mg / ml; solvent is PBS buffer mixed with an equal volume of ethanol; concentration of penetration enhancer and combination of penetration enhancer (w / w) and formulation See the table below:
[0026] Table 1 Concentration and ratio of penetration enhancers and combinations
[0027]
[0028] Instruments: waveform converter (Agilent33120A, PaloAlto, California); multimeter (Fluke189, Everett, Washington); multifunctional microplate detector (BioTek SynergyTM H4); constant temperature water bath; micropipette; Ag / AgCl electrode (Invivo Metrics, Healdsberg, CA, USA).
[0029] 1.2 Operation steps
[0030] 1.2.1 Assembly of diffusion cell
[0031] Two 3cm×3cm, 1.2cm thick Teflon-coated sieve plates were used as the top plate and the bottom p...
Embodiment 2
[0039] The selection of the effective measurement time of embodiment 2 conductivity
[0040] The experimental materials, reagents and operation steps are the same as in Example 1.
[0041] Select the combination of SLS+DPC with a total concentration of 1%, and measure the conductance at 0h, 2h, 4h, 8h, 16h and 24h for 4 samples according to the ratio of 0:1, 1:3, 1:1 and 1:0 Rate Enhancement Index ER. Such as Figure 4 As shown, the 2h, 4h, 8h, 16h and 24h tests can all reflect the differences in the transdermal transmission of inulin in the four samples, that is, the tests at 2-24 hours can all characterize the penetration enhancer and the combination.
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Abstract
The invention aims to provide a high-pass method for evaluating the percutaneous absorption efficiency of an active component of a cosmetic in vitro. The method comprises the following steps of: assembling a high-pass diffusion cell; preparing a sample and processing the sample; measuring the conductivity of a sample cell; and carrying out statistic analysis on data. The high-pass diffusion cell is of a sandwich structure formed in such a way that two Teflon coating sieve pore plates with the area of (3-5cm)*(3-5cm) and the thickness of 1-1.2cm are respectively used as a top plate and a bottom plate, bionic skin or pigskin is used as a middle interlayer, and the four corners are fixed by using bolts; and the sieve pore plates are provided with 24-100 pores which are arranged uniformly and have the diameters of 3-10mm, and the sieve pore plates are respectively used as a donor cell and a receptor cell. The number of single chambers of a Franz diffusion cell is increased to 24-100, therefore, the efficiency is increased by 24-100 times; and the permeability is replaced with the conductivity, so that the detection time is greatly shortened, and the detection efficiency is further increased by over 12 times.
Description
technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a detection method for transdermal absorption efficiency of cosmetic active ingredients, in particular to a high-throughput screening method for a penetration enhancer and its combination. Background technique [0002] In the field of cosmetic science, the development of nutrition, moisturizing, anti-aging, whitening and other functional cosmetics is to meet people's higher-level demands for beauty; but the addition of simple raw materials may not be able to play the role of active ingredients, and may also breed bacteria and cause skin damage. Negative effects such as infection. Therefore, the research and development of functional cosmetics should also pay attention to the percutaneous absorption of cosmetics, and reasonably evaluate the targeting, effective concentration and duration of active ingredients on the action site. The percutaneous absorption of cosmetics refers t...
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