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Corn source assisted memory enhancing peptide and preparation method thereof

An auxiliary enhancement and memory technology, applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of activity decline, loss, and impact on the research process of serial products, etc., to achieve significant improvement of advantages and less investment in equipment , Promote the effect of comprehensive utilization and industrial development

Active Publication Date: 2014-08-27
吉林省圣恒电子商务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the possibility of degradation of peptides due to oxidation, deamidation, hydrolysis or cyclization in the process of production, application and storage, it may also lead to a decrease in activity or even a complete loss, which seriously affects the further development of serialized products. research process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Put corn gluten powder with a protein content of 66% in a drying oven for dehydration and drying, the drying temperature is controlled at 35° C., and dried for 10 hours until the moisture content of corn gluten powder is 1.5%. Use a superfine pulverizer to pulverize the material, and the specification of the pulverizing screen is 200 mesh, collect the superfine powder of zein, and quickly seal it for later use. Use corn gluten puffed powder as the substrate, adjust the concentration of the substrate to 5% (W / W) with pure water, and perform homogenization treatment with a high-speed dispersing homogenizer. The control speed is 3000r / min, and the processing time is 10min; Heating and denaturing in a water bath at 90°C for 10 minutes, cooling to control the enzymatic hydrolysis temperature at 50°C, adjusting the pH value to 9.1, adding enzyme activity to 1.2 million u / g of liquid alkaline protease, use 1M NaOH to control the change of the pH value of the feed s...

Embodiment 2

[0037] Example 2: Put corn gluten powder with a protein content of 66% in a drying oven for dehydration and drying, the drying temperature is controlled at 40°C, and dried for 10 hours until the moisture content of the puffed corn gluten powder is 1.5%. Use a superfine pulverizer to pulverize the material, and the specification of the pulverizing screen is 200 mesh, collect the superfine powder of zein, and quickly seal it for later use. Use corn protein puffed powder as the substrate, adjust the substrate concentration to 5% (W / W) with deionized water, and perform homogenization treatment with a high-speed dispersing homogenizer, control the speed at 3000r / min, and process for 10 minutes; Place it in a water bath at 90°C for 10 minutes, heat and denature it for 10 minutes, cool down to control the enzymatic hydrolysis temperature at 45°C, adjust the pH value to 10.2, add the enzyme activity to 1.2 million u according to [E] / [S] at 5% (W / W) / g of liquid alkaline protease, use ...

Embodiment 3

[0038]Example 3: Put corn gluten powder with a protein content of 66% in a drying oven for dehydration and drying, the drying temperature is controlled at 40° C., and dried for 10 hours until the moisture content of the puffed product of corn gluten powder is 1.5%. The material is crushed with a superfine pulverizer, and the size of the crushing screen is 180 mesh, and the ultrafine powder of zein is collected and quickly sealed for use. Use corn gluten puffed powder as the substrate, adjust the substrate concentration to 4% (W / W) with pure water, and perform homogenization treatment with a high-speed dispersing homogenizer, control the speed at 3000r / min, and process for 10 minutes; Heating and denaturing in a water bath at 90°C for 10 minutes, cooling to control the enzymatic hydrolysis temperature at 45°C, adjusting the pH value to 8.4, adding enzyme activity to 1.2 million u / g of liquid alkaline protease, use 1M NaOH to control the change of the pH value of the feed solut...

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PUM

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Abstract

The invention belongs to the technical field of deep processing of corn and comprehensive utilization of by-products of the corn and discloses a corn source assisted memory enhancing peptide and a preparation method thereof. The corn source assisted memory enhancing peptide is prepared by utilizing corn protein powder as raw material and designing a process route comprising enzymolysis, ultrafiltration, steady-state treatment, synergistic treatment, vacuum rotary evaporation and vacuum freeze drying, and the like; with experiment results of animal behavior test water maze, shuttle box, jungle gym, exploratory heading and diving platform as measurement indexes, optimal process parameters of enzymolysis technology, steady state technology and synergistic technology are optimized so as to greatly improve the experiment result advantages of an tested mice experimental group compared with a control group (P is smaller than 0.01). The invention is aimed at deeply researching the biological functions of corn processing by-products, improving the comprehensive utilization level of corn resources and accelerating the development process of series of products in corn deep processing.

Description

technical field [0001] The invention belongs to the technical field of deep processing of corn and comprehensive utilization of its by-products, and relates to a method for preparing corn source-assisted enhanced memory peptides by using alkaline protease enzymolysis technology, ultrafiltration technology, stabilization technology and synergistic technology. With the support of optimal enzymatic hydrolysis technology, steady-state technology and efficiency-enhancing technology, a kind of corn-derived auxiliary enhanced memory peptide product was prepared. According to animal experiments, compared with the control group, the mice in the experimental group The water maze travel time was shortened by 24%, the number of electric shocks in the shuttle box was reduced by 18%, the pole climbing time was shortened by 26%, the number of cave exploration was increased by 19%, and the reaction period of platform jumping was shortened by 29% (P<0.01). Background technique [0002] Co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 林松毅王可刘旭野杨帅伶张秦溥肖轲刘静波
Owner 吉林省圣恒电子商务有限公司
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