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Small molecular polypeptide ZY13 and application thereof

A small molecule peptide and molecular weight technology, applied in the field of biomedicine, can solve the problems of complex antibacterial peptide antibacterial mechanism, leakage of bacterial content, permeability change, etc., and achieve significant therapeutic effect, growth inhibition, and small molecular weight.

Active Publication Date: 2013-09-04
SICHUAN HETAI SYNLIGHT BIOTECH LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The antibacterial mechanism of antimicrobial peptides is complex, but most of the theories believe that the mechanism involves the interaction between the cationic and hydrophobic properties of antimicrobial peptides and negatively charged microbial cell membranes. After the antimicrobial peptides come into contact with bacterial cell membranes, they cause changes in membrane permeability. , or form a transmembrane hole in the bacterial cell membrane, and finally cause the bacterial content to leak out and die

Method used

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  • Small molecular polypeptide ZY13 and application thereof
  • Small molecular polypeptide ZY13 and application thereof
  • Small molecular polypeptide ZY13 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Preparation of small molecule polypeptide ZY13

[0019] 1. Weigh 0.2g of resin and place it in a dry and clean reaction tube, add an appropriate amount of N,N-dimethylamide (DMF) and activate for 30 minutes. Weigh 1 mmol of the first amino acid residue, add 150 mg of 4-dimethylaminopyridine (DMAP) into the reaction tube, and react with DMF for 3 hours as a solvent. After the reaction is completed, wash with DMF for 3-6 times, and add appropriate pyridine and acetic anhydride , the volume ratio is 1:1, react for 30 minutes, and wash with DMF 3-6 times after the reaction is completed. Then use piperidine to elute the protective group Fmoc of the amino acid, elution twice, each 15min, then wash 4 times with DMF, wash 2 times with methanol;

[0020] 2. Weigh 3mmol of the second amino acid and 3mmol of HBTU in a reaction tube, add 0.5ml of DIEA, react for 40min, wash with DMF for 3-6 times, add piperidine solution to elute the amino acid protecting group Fmoc twi...

Embodiment 2

[0023] Example 2: Antibacterial experiment of small molecule polypeptide ZY13

[0024] 1. Prepare Escherichia coli, Candida albicans, Staphylococcus aureus, Bacillus subtilis, Staphylococcus hemolyticus, Staphylococcus epidermidis, and Staphylococcus coli respectively, culture at 37°C for 18 hours, and set aside;

[0025] 2. Prepare the small molecular polypeptide ZY13 solution with a concentration of 0.8-20 μg / ml, sterilize and dry a circular qualitative filter disc with a diameter of 5-7 mm, and then immerse it in the above-mentioned small molecular polypeptide ZY13 solution with different concentrations;

[0026] 3. Prepare high-column broth agar medium, sterilize, and set aside;

[0027] 4. Melt the tall column broth agar medium, cool to 50°C, add Escherichia coli, Candida albicans, Staphylococcus aureus, Bacillus subtilis, hemolytic Staphylococcus, Staphylococcus epidermidis, and Staphylococcus kosheri respectively 1ml , shake evenly, pour into a sterile plate, and shake...

Embodiment 3

[0036] Example 3: Therapeutic effect of small molecule polypeptide ZY13 on mouse ear acne model

[0037] 1. Cultivate Propionibacterium acnes ATCC11817 with brain heart infusion medium, cultivate to logarithmic phase, wash with normal saline twice, and resuspend in normal saline to 5×10 8 CFU / ml;

[0038] 2. Select male mice with a weight of 18g, Kunming species, and randomly divide them into 3 groups, 6 mice in each group. Anesthetized with sodium, and then intradermally injected 20 μl / mouse of the resuspended bacteria solution into the left ear of the mouse;

[0039] 3. The small molecule polypeptide ZY13 and clindamycin were prepared into 2 mg / ml ointment with polyethylene glycol and glycerol, respectively, wherein the weight ratio of polyethylene glycol and glycerin was 5:1, and rubbed on the skin of the left ear of the mouse On the surface, the small molecule polypeptide ZY13 was applied in the treatment group, clindamycin was applied in the positive control group, and ...

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Abstract

The invention discloses small molecular polypeptide ZY13 and an application thereof in preparing acne treatment medicine, anti-infection and anti-tumor medicines. The small molecular polypeptide ZY13 comprises 15 amino acid residues, the molecular weight is 2228.77Da, the isoelectric point is 12.05, and the amino acid sequence is represented in SEQID: 1. The polypeptide ZY13 is synthesized artificially, has small molecular weight, is convenient to synthesize artificially, has obvious antibacterial effect, and has remarkable curative effect on acne; the polypeptide can further refrain growth of tumor cells, and has a killing function on the tumor cells; and the small molecular polypeptide ZY13 can be applied to preparation of anti-infection, anti-tumor and acne treatment medicines.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of small molecular polypeptide ZY13 in the preparation of acne, anti-infection and anti-tumor drugs. Background technique [0002] Acne is a chronic inflammation of the pilosebaceous glands. It is a common inflammatory skin disease in adolescence. It usually occurs on the cheeks, forehead, cheeks and nasolabial folds, followed by the chest, back and shoulders. Its onset is mainly related to sex hormones. acne levels, massive secretion of sebaceous glands, proliferation of Propionibacterium acnes, abnormal keratinization of pilosebaceous ducts, and inflammation. During adolescence, the lipid composition of the skin surface changes, and the male hormone increases, resulting in hyperkeratosis of the sebaceous follicle duct, and the epithelial cells shed on the hair follicle wall are mixed with sebum, embolized in the hair follicle mouth, forming acne, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P17/10A61P31/00A61P35/00
CPCA61K38/00A61P17/10A61P31/00A61P35/00C07K7/08
Inventor 杜彦军刘音赖仞容明强
Owner SICHUAN HETAI SYNLIGHT BIOTECH LTD
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