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Detection test paper strip of colloidal gold method for adenovirus IgM and IgG antibodies, kit and preparation method thereof

A detection kit and detection test paper technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of inconvenient and fast detection, cumbersome operation process, etc., and achieve the effects of simple detection, simplified operation process and high sensitivity

Inactive Publication Date: 2013-08-28
JIANGSU KEYGEN BIOTECH CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the individual detection of IgM and IgG antibodies, and then a comprehensive analysis of the test results has no effect on the diagnosis of the disease, but the operation process is cumbersome and the detection is not convenient and quick

Method used

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  • Detection test paper strip of colloidal gold method for adenovirus IgM and IgG antibodies, kit and preparation method thereof
  • Detection test paper strip of colloidal gold method for adenovirus IgM and IgG antibodies, kit and preparation method thereof
  • Detection test paper strip of colloidal gold method for adenovirus IgM and IgG antibodies, kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Composition of Adenovirus IgM, IgG Antibody Detection Kit (Colloidal Gold Method)

[0034] 1. Sample processing bottle: The sample processing bottle (purchased from Hangzhou Beiyu) is filled with 1mL sample processing solution (phosphate buffer solution, the main raw material is purchased from Sinopharm Group).

[0035]2. Test strip: 60*5mm bottom plate (purchased from Shanghai Jieyi Company); 15*5mm thick fiber filter paper for absorbent pad (purchased from Shanghai Jieyi Company); 25*5mm nitrocellulose membrane (purchased from Millipore Company ) were sequentially coated with mouse anti-human IgG antibody (purchased from ABD Company), mouse anti-human IgM antibody (purchased from ABD Company), rabbit anti-Adv polyclonal antibody (purchased from ABCAM Company); sample pad (purchased from Shanghai Jieyi Company ) was attached with a whole blood filtration membrane (purchased from Shanghai Jieyi Company); the gold standard pad (purchased from Shanghai Jieyi Co...

Embodiment 2

[0036] Embodiment 2: the preparation of kit

[0037] 1. Preparation of sample processing solution: Prepare sample processing solution: prepare phosphate buffer solution (pH7.0±0.2), dispense it into sample processing bottles, 1mL / bottle.

[0038] 2. Preparation of test strips:

[0039] 2.1. Dilute the mouse anti-human IgG antibody, mouse anti-human IgM antibody, and rabbit anti-Adv polyclonal antibody to a coating concentration of 1.0-1.2 mg / mL, and draw the three coating solutions on the designated positions on the nitrocellulose membrane respectively. The coated nitrocellulose membrane was dried and stored at 37°C for 4 hours.

[0040] 2.2. Preparation of gold standard pad: Colloidal gold was prepared by trisodium citrate reduction method, and recombinant Adv antigen was slowly added to the gold sol at a ratio of 20 μg / mL to 40 μg / mL. After sufficient reaction, it was centrifuged at 8000 RPM for 30 minutes, and discarded. Add the diluent to 1 / 3 of the original volume, then...

Embodiment 3

[0044] Embodiment 3 detection method

[0045] 1. Test samples according to clinical routine, and the test samples can be serum. If the sample cannot be detected within three days, it can be stored at 2-8°C, and the storage period should not exceed 7 days.

[0046] 2. Take 100-200ul of serum and add it dropwise to the sample window.

[0047] 3. Interpret the result after 10-15 minutes:

[0048] 3.1. If the sample contains adenovirus IgG antibody, it will combine with the recombinant Adv antigen-colloidal gold complex when it passes through the gold label pad, and then be captured by the mouse anti-human IgG antibody at T1, and aggregate to form wine red detection Wire.

[0049] 3.2. If the sample contains adenovirus IgM antibody, it will combine with the recombinant Adv antigen-colloidal gold complex when it passes through the gold label pad, and then be captured by the mouse anti-human IgM antibody at T2, and aggregate to form wine red detection line; see attached image ...

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Abstract

The invention provides a detection test paper strip of a colloidal gold method for adenovirus IgM and IgG antibodies, a kit and a preparation method thereof. The invention can determine the IgM and IgG antibodies by an immunocapture method principle, and can co-detect specific anti-Adv IgM and IgG antibodies by once operation, thereby simplifying an operation process. The kit is used for simple, rapid and accurate detection, reduces a lot of complex programs, needs neither a special instrument nor special training, is particularly suitable for a primary department, and can make an auxiliary diagnosis effect for early stage or mid-stage adenovirus infection.

Description

technical field [0001] The invention belongs to the field of biological detection, and relates to a test strip, a test kit and a preparation technology for detecting specific IgM and IgG antibodies by immunoassay; The invention relates to a test strip, a test kit and a preparation method thereof for combined detection of adenovirus IgM and IgG antibodies. Background technique [0002] Immunoglobulin M (Immunoglobulin M, IgM) and Immunoglobulin G (Immunoglobulin G, IgG) are the two most important antibodies in humans or animals. After an antigen (bacteria or virus, etc.) enters the body, the first immunoglobulin that appears is IgM antibody, followed by IgG antibody. Although IgA antibody may also appear in some cases, because its clinical significance is basically the same as that of IgG, and the IgA content is very low, it has little practical significance in clinical testing, so it is generally not distinguished in clinical testing. By detecting the presence of IgM and I...

Claims

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Application Information

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IPC IPC(8): G01N33/569
Inventor 不公告发明人
Owner JIANGSU KEYGEN BIOTECH CORP LTD
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