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Pt nano flower micro needle type enzyme-free glucose sensor electrode and preparation method thereof

A glucose sensor and nanoflower technology, applied in the field of biosensors, can solve the problems of low binding force, low activity of inert electrodes, easy to fall off, etc., and achieve the effects of improved stability, favorable performance and good electrocatalytic performance.

Inactive Publication Date: 2013-08-21
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to meet the miniaturization requirements of the dynamic blood glucose detection system, the working electrode of the sensor must use an inert needle electrode with a small area. enzyme sensitive membrane
[0004] Using traditional electrodeposition technology to deposit glucose molecular sensitive film on the surface of the needle electrode, the bonding force between the deposited film and the stainless steel needle substrate is small and easy to fall off

Method used

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  • Pt nano flower micro needle type enzyme-free glucose sensor electrode and preparation method thereof
  • Pt nano flower micro needle type enzyme-free glucose sensor electrode and preparation method thereof
  • Pt nano flower micro needle type enzyme-free glucose sensor electrode and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] The silver alloy needle electrode was soaked in acetone, and ultrasonically cleaned to remove surface oil, then repeatedly cleaned with ethanol, ultrasonically, dried in the air, placed in a PBS solution with a pH value of 7.0, and activated by electrochemical cyclic voltammetry scanning for 10 lock up. Then it was placed in an electrolyte solution containing 0.5M sulfuric acid and 3mM chloroplatinic acid, using a three-electrode system, with a platinum electrode as an auxiliary electrode, a saturated calomel electrode as a reference electrode, and a pretreated silver alloy needle electrode as The working electrode was placed in an ultrasonic cleaning device, and the Pt nanoflower enzyme-free needle electrode was prepared by ultrasonic oscillation constant potential electrodeposition. The electrodeposition potential was -0.2V, the electrodeposition time was 600s, the electrodeposition temperature was 25°C, and ultrasonic The vibration power is 20W, and the interval time...

Embodiment 2

[0034] The operation process and experimental conditions of Example 2 are the same as those of Example 1, except that the ultrasonic intermittent oscillation electrodeposition potential is changed to -0.1V, and the electrodeposition time is 1000s.

[0035] The prepared electrode was used as the working electrode, the Pt wire electrode was used as the auxiliary electrode, and the saturated calomel electrode was used as the reference electrode. Into 30ml of blank PBS, 30μL of 1.5mol / L glucose solution was added dropwise successively, and after each dropwise addition, differential pulse voltammetry (DPV) measurement was carried out. figure 2 The results showed that the sensitivity of the prepared enzyme-free sensor was 1.87μA(cm -2 mM -1 ), the linear monitoring range is 1mM-16mM, and the detection limit is 48μM. After placing the electrode for three months, the response current is 92% of the initial response current. The sensor has very good stability, compared with the corres...

Embodiment 3

[0037] The operating process and experimental conditions of Example 3 are the same as in Example 1, except that the concentration of sulfuric acid is changed to 0.1M, and the concentration of chloroplatinic acid to 2mM.

[0038] The prepared electrode was used as the working electrode, the Pt wire electrode was used as the auxiliary electrode, and the saturated calomel electrode was used as the reference electrode. Into 30ml of blank PBS, 30μL of 1.5mol / L glucose solution was added dropwise successively, and after each dropwise addition, differential pulse voltammetry (DPV) measurement was carried out. image 3 The results show that the sensitivity of the prepared sensor is 0.53μA*(cm -2 mM -1 ), the linear monitoring range is 1-15mM, and the detection limit is 169μM. After placing the electrode for three months, the response current is 91% of the initial response current. Compared with the corresponding pin-type enzyme sensor (performance see figure 1 ), wider linear range...

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Abstract

The invention discloses a Pt nano flower micro needle type enzyme-free glucose sensor electrode and a preparation method thereof. The electrode is characterized by being composed of a pt nano flower, wherein average length of single pedal is 100nm-200nm, and average width of single pedal is 50nm-100nm. The Pt nano flower micro needle type enzyme-free glucose sensor electrode is prepared by using an ultrasonic intermittent constant potential electrolytic deposition method, the preparation method is simple in process, the reaction conditions are easy to control, and the binding force between the Pt nano flower and the needle electrode is strong. The preparation method has the advantage that the enzyme-free glucose sensor electrode can be formed on the micro needle electrode surface, wherein the electrode has better electrocatalytic oxidation performance to the glucose, and the formed micro needle type enzyme-free glucose sensor has a wide linearity range, high response sensitivity, low detection limit and high stability for the glucose, and has better application foreground in the aspect of implanted glucose sensors.

Description

technical field [0001] The invention relates to the field of biosensors, in particular to a Pt nanoflower micro-needle enzyme-free glucose sensor electrode and a preparation method thereof. Background technique [0002] Continuous blood glucose monitoring has important clinical significance for the treatment of diabetic patients and the screening of early childhood diabetes. However, invasive blood glucose detectors cannot continuously monitor blood glucose concentration. The existing dynamic blood glucose detection system uses glucose oxidase as a sensitive substance. The enzyme is easily inactivated and denatured during immobilization and use, which leads to the stability of the sensor The second is that the amount of immobilized enzyme and the influence on the enzyme activity during the immobilization process cannot be accurately controlled, so that the repeatability of the prepared sensor cannot be guaranteed. In the long-term clinical practice of the existing blood glu...

Claims

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Application Information

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IPC IPC(8): G01N27/30
Inventor 郭美卿戴震陈静史振东武晓刚
Owner TAIYUAN UNIV OF TECH
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