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Combined production method for abalone polysaccharide, lipid and protein peptide

A technology of abalone polysaccharides and protein peptides, applied in the field of extracting abalone polysaccharides, lipids and protein peptides, can solve the waste of abalone resources, the economic value and function of abalone viscera are not fully reflected, and the extraction and utilization of abalone protein peptides and lipids are ignored and other issues, to achieve the effects of reducing production costs, easy absorption, and reducing emissions

Active Publication Date: 2013-08-21
XIAMEN DAOZHIYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2012, the annual output of abalone in my country has reached 80,000 tons, and in line with the needs of market development, the abalone processing industry has also begun to show a trend of vigorous development. During the processing process, visceral by-products accounting for about 1 / 4 of abalone will inevitably be produced, but at present Abalone offal is mainly used to produce low value-added products such as feed and seasoning, which seriously wastes precious abalone resources
Because abalone viscera is similar to abalone muscle, rich in polysaccharides, proteins and lipids, Maillard reaction is prone to occur during the extraction of abalone polysaccharides, and the color of the product will appear brown (patent application number: 200510047409.X). The color will turn yellow, and most domestic and foreign scholars pay attention to the extraction of abalone polysaccharides, ignoring the extraction and utilization of abalone protein peptides and lipids, and the economic value and function of abalone viscera have not been fully reflected

Method used

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  • Combined production method for abalone polysaccharide, lipid and protein peptide
  • Combined production method for abalone polysaccharide, lipid and protein peptide
  • Combined production method for abalone polysaccharide, lipid and protein peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Enzymolysis: Add 1L of pure water to 1kg of cleaned abalone viscera, adjust the pH to 8.0 with alkali, add 1g of trypsin and 1g of collagenase, stir and incubate at 45°C for 6 hours, then Adjust the pH to 7.0 with acid, add 10 g of neutral protease, stir and insulate the enzymolysis at 45°C for 6 hours, filter and remove insoluble matter, and obtain the enzymatic hydrolysis solution of abalone viscera.

[0030] (2) Membrane separation: the obtained abalone viscera enzymolysis solution is separated by a 5000 Dalton ultrafiltration membrane, the enzymolysis solution intercepted by the ultrafiltration membrane is a concentrated solution, and the enzymolysis solution passing through the ultrafiltration membrane is a dialysis solution. liquid.

[0031] (3) Alcohol precipitation: Add 2.5L of 99.5% alcohol to the 850ml concentrated solution obtained by the ultrafiltration membrane, let it settle at room temperature for 8 hours, and obtain 105g of polysaccharide precipitate...

Embodiment 2

[0036] (1) Enzymolysis: Add 1L of pure water to 1kg of cleaned abalone viscera, adjust the pH to 9.0 with alkali, add 5g of trypsin and 5g of collagenase, stir and incubate at 50°C for 4 hours, then Adjust the pH to 6.0 with acid, add 20 g of bromelain, stir and incubate the enzyme for 3 hours at 50°C, then filter and remove insoluble matter to obtain the enzymolysis solution of abalone viscera.

[0037] (2) Membrane separation: The obtained abalone viscera enzymatic solution is separated by a 10,000-dalton ultrafiltration membrane. The enzymatic solution intercepted by the ultrafiltration membrane is a concentrated solution, and the enzymatic solution that passes through the ultrafiltration membrane is a dialysis solution. liquid.

[0038](3) Alcohol precipitation: Add 3.0L of 99.5% alcohol to the 800ml concentrated solution obtained by the ultrafiltration membrane, let it stand at room temperature for 10 hours, and obtain 100g of polysaccharide precipitation by centrifugatio...

Embodiment 3

[0043] (1) Enzymolysis: Add 1L of pure water to 1kg of cleaned abalone viscera, adjust the pH to 8.5 with alkali, add 10g of trypsin and 10g of collagenase, stir and incubate at 50°C for 6 hours, then Adjust the pH to 5.0 with acid, add 5g of bromelain, stir and incubate the enzymolysis at 50°C for 3 hours, filter and remove insoluble matter, and obtain the enzymolysis solution of abalone viscera.

[0044] (2) Membrane separation: The obtained abalone viscera enzymatic solution is separated by a 20,000 Dalton ultrafiltration membrane. The enzymatic solution retained by the ultrafiltration membrane is a concentrated solution, and the enzymatic solution that passes through the ultrafiltration membrane is a dialysis solution. liquid.

[0045] (3) Alcohol precipitation: Add 3.0L of 99.5% alcohol to the 750ml concentrated solution obtained by the ultrafiltration membrane, let it stand at room temperature for 12 hours, and obtain 107g of polysaccharide precipitate by centrifugation ...

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Abstract

The invention discloses a combined production method for abalone polysaccharide, lipid and protein peptide. According to the technological process, the abalone polysaccharide, lipid and protein peptide are prepared by carrying out composite enzymatic hydrolysis, membrane separation, alcohol precipitation, centrifugal separation, freezing crystallization, decoloration, drying and the like on abalone viscera which is taken as a raw material. The combined production method is simple in process, the deficiency that raw materials are utilized insufficiently in a traditional technology can be improved, the emission of wastes in the preparation process can be reduced, and the production cost can be lowered. The abalone polysaccharide and lipid extracted can be taken as intermediate raw materials to research and develop all novel functional foods; and the abalone protein peptide prepared is colorless and tasteless, and the molecular weight is mainly concentrated near 350 dalton, so that the abalone protein peptide is easily absorbed by a human body, and can be widely applied to industries of health-care food, biomedicines, cosmetics and the like relating to human health.

Description

technical field [0001] The present invention relates to a co-production preparation method of abalone polysaccharides, lipids and protein peptides, in particular to a method for extracting abalone polysaccharides, lipids and proteins from abalone viscera by using controlled enzymatic hydrolysis, membrane separation and freeze crystallization peptide method. Background technique [0002] Abalone is a valuable seafood, rich in nutrition, rich in arginine, alanine, glutamic acid, vitamin E, trace elements and other physiologically active substances, which play an important role in maintaining the body's acid-base balance and neuromuscular excitement . In 2012, the annual output of abalone in my country has reached 80,000 tons, and in line with the needs of market development, the abalone processing industry has also begun to show a trend of vigorous development. During the processing process, visceral by-products accounting for about 1 / 4 of abalone will inevitably be produced,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04C12P21/02
Inventor 翁武银
Owner XIAMEN DAOZHIYUAN BIOTECH
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