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Application of Aspeverin in preparation of medicines for inhibiting liver fibroblast proliferation

A technology of fibroblasts and drugs, which is applied in the field of application of Aspeverin in the preparation of drugs that inhibit the proliferation of liver fibroblasts, and can solve problems such as the lack of clinical treatment methods

Inactive Publication Date: 2013-08-21
NANJING ZHENGLIANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, some anti-hepatic fibrosis treatments have appeared successively, including chemical drugs, biological agents, traditional Chinese medicine and gene therapy, etc., but the ideal clinical treatment methods are still lacking (Liu Ping. Strengthen the research on the mechanism of anti-hepatic fibrosis. Chinese liver disease Magazine, 2005, 8(13): 561)

Method used

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  • Application of Aspeverin in preparation of medicines for inhibiting liver fibroblast proliferation
  • Application of Aspeverin in preparation of medicines for inhibiting liver fibroblast proliferation
  • Application of Aspeverin in preparation of medicines for inhibiting liver fibroblast proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Preparation of the compound Aspeverin tablet of the present invention:

[0015] Take 20 grams of compound Aspeverin, add 180 grams of conventional excipients for preparing tablets, mix well, and use a conventional tablet machine to form 1000 tablets.

Embodiment 2

[0016] Example 2: Preparation of the compound Aspeverin capsules involved in the present invention:

[0017] Take 20 grams of compound Aspeverin, add 180 grams of conventional adjuvants for preparing capsules such as starch, mix well, and encapsulate to make 1000 capsules.

[0018] The pharmacodynamics experiment will further illustrate its drug activity below.

experiment example 1

[0019] Experimental example 1: MTT method was used to evaluate the inhibitory effect of compound Aspeverin on liver fibrosis

[0020] 1. Methods: The NIH / 3T3 cell line was purchased from the cell bank of the Shanghai Institute of Cell Research, Chinese Academy of Sciences, which was quoted from ATCC (American Type Culture Collection). Subconfluent NIH / 3T3 cells in logarithmic growth phase were digested with 0.25% trypsin, washed, centrifuged, and made into 1 × 10 cells in DMEM medium (containing 10% FCS). 4 cell / ml cell suspension, trypan blue staining to identify the viability of more than 95%, add 100ul per well to a 96-well plate, at 37°C, 5% CO 2 After 24 hours of synchronous treatment, the supernatant was discarded, 200 ul of DMEM medium (containing 10% FCS) containing drugs at different dilution concentrations was added, and 48 hours of culture were added, and MTT solution was added to each well to incubate for 4 hours. Discard the culture medium, add 150ul DMSO, shake ...

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Abstract

The invention discloses an application of Aspeverin in preparation of medicines for inhibiting liver fibroblast proliferation. Pharmacological testing proves that the Aspeverin can obviously inhibit liver fibrosis from being caused and developed, and can be used for preparing the medicines for resisting liver fibrosis and treating liver cirrhosis. The application of Aspeverin in preparation of medicines for inhibiting liver fibroblast proliferation is disclosed for the first time, a framework type belongs to a brand-new framework type, the Aspeverin has an unexpected strong effect of inhibiting the liver fibroblast proliferation activity, and the revelation possibility of other compounds cannot be generated, so that the application has obvious substantive features; and moreover, the application has an obvious improved effect of inhibiting the liver fibroblast proliferation.

Description

technical field [0001] The invention relates to a new use of the compound Aspeverin, in particular to the application of Aspeverin in the preparation of a drug for inhibiting the proliferation of liver fibroblasts. Background technique [0002] Liver fibrosis is a dynamic process from chronic liver injury to liver cirrhosis, which is characterized by a large amount of extracellular matrix (ECM) synthesis and secretion, while the degradation is absolutely or relatively insufficient, resulting in diffuse deposition of ECM in the liver. It starts with necrosis of hepatocytes (HC), followed by inflammatory response, release of fibrogenesis mediators, activation of hepatic stellate cells (FSC), and finally the synthesis and degradation of connective tissue components in the liver are obviously out of balance. Liver fibrosis is a common pathological process of many chronic liver diseases and an important factor affecting prognosis. [0003] In the past 20 years, the research on l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/537A61P1/16
Inventor 不公告发明人
Owner NANJING ZHENGLIANG MEDICAL TECH
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