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Preparation method for steelhead trout and brook trout hybrid triploid seed

A technology of steelhead trout and triploid, which is applied in the field of preparation of the hybrid triploid seed of steelhead trout and brook char, and can solve the problem of sensitivity to chemical substances, high requirements on water quality of the growth environment, and no 6-diploid species. Methylaminopurine, etc.

Inactive Publication Date: 2014-12-24
BEIJING FISHERIES RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 6-Dimethylaminopurine (N6-Dimethyladenine, 6-DMAP) has achieved good results in the induction of polyploidy in various shellfish, but because salmon trout have high requirements on the water quality of the growth environment and are particularly sensitive to chemical substances, So far, there is no successful report on the application of 6-dimethylaminopurine in the induction of triploid or hybrid triploid in salmon and trout

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  • Preparation method for steelhead trout and brook trout hybrid triploid seed
  • Preparation method for steelhead trout and brook trout hybrid triploid seed

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1 Preparation method of hybrid triploid seed of steelhead trout and brook trout (1)

[0027] Stimulation by water flow, light control, and enhanced compound feed for broodstock were used to make broodstock of steelhead trout and broodstock of brook trout develop and mature synchronously. The water temperature for broodstock breeding of steelhead trout was 10°C, and the water exchange rate in the running pool was 2h / time.

[0028] The eggs are fertilized by dry method. Squeeze the roe of mature steelhead female fish into a clean porcelain basin first, and then squeeze the semen of 2 male brook trout into the water basin. The water temperature is 10°C, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1:2500, stir gently, pour out the water after 1 min, repeat 3 times, clean the semen, and place it for processing.

[0029] After 12 minutes of fertilization, the fertilized eggs were quickly transferred to the 6-DMAP ...

Embodiment 2

[0031] Embodiment 2 Preparation method of hybrid triploid seed of steelhead trout and brook trout (2)

[0032] Stimulation by water flow, light control, and enhanced compound feed for broodstock were used to make steelhead trout and broodstock broodstock mature synchronously. The water temperature for broodstock breeding of steelhead trout was 13°C, and the water exchange rate in the running pool was 2h / time.

[0033] The eggs are fertilized by dry method. Squeeze the roe of mature steelhead female fish into a clean porcelain basin first, and then squeeze the semen of 2 male brook trout into the water basin. The water temperature is 8°C, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1:3000, stir gently, pour out the water after 1.5 minutes, repeat 3 times, clean the semen, and place it for treatment.

[0034] After 13 minutes of fertilization, the fertilized eggs were quickly transferred to the 6-DMAP solution with a concentrat...

Embodiment 3

[0036] Embodiment 3 Preparation method of hybrid triploid seed of steelhead trout and brook trout (3)

[0037]Stimulation by water flow, light control, and enhanced compound feed for broodstock were used to make steelhead trout and broodstock broodstock mature synchronously. The water temperature for broodstock breeding of steelhead trout was 11°C, and the water exchange rate in the running pool was 2h / time.

[0038] The eggs are fertilized by dry method. Squeeze the roe of mature steelhead female fish into a clean porcelain basin first, and then squeeze the semen of 2 male brook trout into the water basin. The water temperature is 9°C, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1.5:3000, stir gently, pour out the water after 1 min, repeat 3 times, clean the semen, and place it for treatment.

[0039] After 14 minutes of fertilization, the fertilized eggs were quickly transferred to the 6-DMAP solution with a concentration o...

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Abstract

The invention provides a preparation method for a steelhead trout and brook trout hybrid triploid seed. The preparation method includes the steps that a fertilized egg is obtained through hybridization of the steelhead trout and the brook trout; the fertilized egg is soaked in 6-DMAP (dimethylanminopyridine) solution, hatched and cultivated; and the hybrid triploid seed is produced. During incubation period, eyed rate of the fertilized egg is 77%, and hatching rate of the seed reaches above 90%. Then the obtained larvae is detected through a flow cytometry, and triploid rate of the obtained larvae reaches above 90%. With the preparation method, large-scale production of the steelhead trout and brook trout hybrid triploid seed is realized, and growth speed and disease-resistant ability of the trout are enhanced, thereby improving economic efficiency for farmers and bringing good market value.

Description

technical field [0001] The invention relates to the technical field of aquatic organism breeding, in particular to a method for preparing hybrid triploid seedlings of steelhead trout and brook trout. Background technique [0002] Salmon trout is one of the main fish cultured in the world at present. Because of its own quality and special requirements for the living environment, it is a green, environmentally friendly and nutritious food, so it is very popular among consumers. Salmon trout is currently the main cold-water cultured fish in my country. With the rapid development of salmon trout farming in China, basic theoretical research is relatively lagging behind. Problems such as the degradation of broodstock germplasm of salmon trout lead to the degradation of seed quality and the decline in production efficiency have gradually become prominent. , thus restricting the sustainable development of salmon and trout farming. Using modern biotechnology to cultivate seedlings wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 徐绍刚李文通田照辉杨晓飞杨贵强
Owner BEIJING FISHERIES RES INST
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